Molecular procedure for detection of Burkholderia pseudomallei.
Recently several cases of melioidosis have been reported in the tropical climates, especially in Southeast Asia where, it is endemic, it also occurs sporadically throughout the world. The diagnosis of the acute or chronic infection remains challenging. The present study highlight on the optimized an...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English English |
| Published: |
Medwell Publishing
2009
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| Online Access: | http://psasir.upm.edu.my/id/eprint/15712/1/Molecular%20procedure%20for%20detection%20of%20Burkholderia%20pseudomallei.pdf |
| _version_ | 1825945674284269568 |
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| author | Lai, L. Suang Sekawi, Zamberi Abdullah Al-Haj, Nagi Ahmed Shamsudin, Mariana Nor Abdullah, Rasedee Mohamed, Rahmah |
| author_facet | Lai, L. Suang Sekawi, Zamberi Abdullah Al-Haj, Nagi Ahmed Shamsudin, Mariana Nor Abdullah, Rasedee Mohamed, Rahmah |
| author_sort | Lai, L. Suang |
| collection | UPM |
| description | Recently several cases of melioidosis have been reported in the tropical climates, especially in Southeast Asia where, it is endemic, it also occurs sporadically throughout the world. The diagnosis of the acute or chronic infection remains challenging. The present study highlight on the optimized and reliable technique based DNA preparation for use in Polymerase Chain Reaction (PCR) assay. PCR amplification with specific pair of primer for each putative gene was proving specific for amplification of genes in Burkholderia pseudomallei strain D286. The PCR mixture with addition of DMSO, formamide and glycerol could ease the PCR optimization where different pairs of primers were involved. The findings of this study have contributed to some information on the molecular bases of the LPS biosynthesis genes in B. seudomallei specifically for strain D286. The specific primer pairs with the PCR mixture could be used in developing a PCR diagnosis of melioidosis. |
| first_indexed | 2024-03-06T07:35:09Z |
| format | Article |
| id | upm.eprints-15712 |
| institution | Universiti Putra Malaysia |
| language | English English |
| last_indexed | 2024-03-06T07:35:09Z |
| publishDate | 2009 |
| publisher | Medwell Publishing |
| record_format | dspace |
| spelling | upm.eprints-157122015-10-05T06:45:27Z http://psasir.upm.edu.my/id/eprint/15712/ Molecular procedure for detection of Burkholderia pseudomallei. Lai, L. Suang Sekawi, Zamberi Abdullah Al-Haj, Nagi Ahmed Shamsudin, Mariana Nor Abdullah, Rasedee Mohamed, Rahmah Recently several cases of melioidosis have been reported in the tropical climates, especially in Southeast Asia where, it is endemic, it also occurs sporadically throughout the world. The diagnosis of the acute or chronic infection remains challenging. The present study highlight on the optimized and reliable technique based DNA preparation for use in Polymerase Chain Reaction (PCR) assay. PCR amplification with specific pair of primer for each putative gene was proving specific for amplification of genes in Burkholderia pseudomallei strain D286. The PCR mixture with addition of DMSO, formamide and glycerol could ease the PCR optimization where different pairs of primers were involved. The findings of this study have contributed to some information on the molecular bases of the LPS biosynthesis genes in B. seudomallei specifically for strain D286. The specific primer pairs with the PCR mixture could be used in developing a PCR diagnosis of melioidosis. Medwell Publishing 2009 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/15712/1/Molecular%20procedure%20for%20detection%20of%20Burkholderia%20pseudomallei.pdf Lai, L. Suang and Sekawi, Zamberi and Abdullah Al-Haj, Nagi Ahmed and Shamsudin, Mariana Nor and Abdullah, Rasedee and Mohamed, Rahmah (2009) Molecular procedure for detection of Burkholderia pseudomallei. Research Journal of Applied Sciences, 4 (2). pp. 73-79. ISSN 1815-932X; ESSN : 1993-6079 http://www.medwelljournals.com/fulltext/?doi=rjasci.2009.73.79 English |
| spellingShingle | Lai, L. Suang Sekawi, Zamberi Abdullah Al-Haj, Nagi Ahmed Shamsudin, Mariana Nor Abdullah, Rasedee Mohamed, Rahmah Molecular procedure for detection of Burkholderia pseudomallei. |
| title | Molecular procedure for detection of Burkholderia pseudomallei. |
| title_full | Molecular procedure for detection of Burkholderia pseudomallei. |
| title_fullStr | Molecular procedure for detection of Burkholderia pseudomallei. |
| title_full_unstemmed | Molecular procedure for detection of Burkholderia pseudomallei. |
| title_short | Molecular procedure for detection of Burkholderia pseudomallei. |
| title_sort | molecular procedure for detection of burkholderia pseudomallei |
| url | http://psasir.upm.edu.my/id/eprint/15712/1/Molecular%20procedure%20for%20detection%20of%20Burkholderia%20pseudomallei.pdf |
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