Morphological abnormality among regenerated shoots of banana and plantain (Musa spp.) after in vitro multiplication with TDZ and BAP from excised shoottips
Five cultivars of Musa spp (banana and plantain); ‘Berangan Intan’, ‘Berangan’ (AAA), ‘Rastali’, ‘Nangka’ (AAB) and ‘Baka Baling’ (ABB) were chosen to compare the effect of benzylaminopurine (BAP) and thidiazuron (TDZ) on multiplication efficiency in relation with frequency of abnormal shoot regener...
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| Format: | Article |
| Language: | English |
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Academic Journals
2009
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| Online Access: | http://psasir.upm.edu.my/id/eprint/15730/1/15730.pdf |
| _version_ | 1825945677792804864 |
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| author | Bidabadi, Siamak Shirani Mahdavi, Fatemeh Mahmood, Maziah |
| author_facet | Bidabadi, Siamak Shirani Mahdavi, Fatemeh Mahmood, Maziah |
| author_sort | Bidabadi, Siamak Shirani |
| collection | UPM |
| description | Five cultivars of Musa spp (banana and plantain); ‘Berangan Intan’, ‘Berangan’ (AAA), ‘Rastali’, ‘Nangka’ (AAB) and ‘Baka Baling’ (ABB) were chosen to compare the effect of benzylaminopurine (BAP) and thidiazuron (TDZ) on multiplication efficiency in relation with frequency of abnormal shoot regeneration. Shoot tips ofMusa spp. were cultured on MS medium supplemented with various concentrations (0.0, 11.1, 22.2, 33.3 and 44.4 µM) of BAP and (0.0, 0.5, 2, 5 and 7.5 µM) of TDZ. Increasing BAP above 22.2 µM and 33.3 µM increased the number of shoots in‘Berangan Intan’, ‘Berangan’, ‘Rastali’, ‘Nangka’ and ‘Baka Baling’ respectively, but above 33.3 µM significantly caused higher gross of abnormal shoot regeneration. TDZ in the media up to 2 µM for ‘Baka Baling’, ‘Nangka’ and ‘Rastali’ and 5 µM for ‘Berangan Intan’ and ‘Berangan’ increased the number of shoots per explant, however TDZ at 5 µM resulted in high number of abnormal shoots. In conclusion BAP at 22.2 µM and TDZ at 2 µM were assumed to be the most suitable for commercial micropropagation system with low frequency of abnormal shoot production for both banana and plantain. |
| first_indexed | 2024-03-06T07:35:12Z |
| format | Article |
| id | upm.eprints-15730 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T07:35:12Z |
| publishDate | 2009 |
| publisher | Academic Journals |
| record_format | dspace |
| spelling | upm.eprints-157302016-05-31T05:41:04Z http://psasir.upm.edu.my/id/eprint/15730/ Morphological abnormality among regenerated shoots of banana and plantain (Musa spp.) after in vitro multiplication with TDZ and BAP from excised shoottips Bidabadi, Siamak Shirani Mahdavi, Fatemeh Mahmood, Maziah Five cultivars of Musa spp (banana and plantain); ‘Berangan Intan’, ‘Berangan’ (AAA), ‘Rastali’, ‘Nangka’ (AAB) and ‘Baka Baling’ (ABB) were chosen to compare the effect of benzylaminopurine (BAP) and thidiazuron (TDZ) on multiplication efficiency in relation with frequency of abnormal shoot regeneration. Shoot tips ofMusa spp. were cultured on MS medium supplemented with various concentrations (0.0, 11.1, 22.2, 33.3 and 44.4 µM) of BAP and (0.0, 0.5, 2, 5 and 7.5 µM) of TDZ. Increasing BAP above 22.2 µM and 33.3 µM increased the number of shoots in‘Berangan Intan’, ‘Berangan’, ‘Rastali’, ‘Nangka’ and ‘Baka Baling’ respectively, but above 33.3 µM significantly caused higher gross of abnormal shoot regeneration. TDZ in the media up to 2 µM for ‘Baka Baling’, ‘Nangka’ and ‘Rastali’ and 5 µM for ‘Berangan Intan’ and ‘Berangan’ increased the number of shoots per explant, however TDZ at 5 µM resulted in high number of abnormal shoots. In conclusion BAP at 22.2 µM and TDZ at 2 µM were assumed to be the most suitable for commercial micropropagation system with low frequency of abnormal shoot production for both banana and plantain. Academic Journals 2009 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/15730/1/15730.pdf Bidabadi, Siamak Shirani and Mahdavi, Fatemeh and Mahmood, Maziah (2009) Morphological abnormality among regenerated shoots of banana and plantain (Musa spp.) after in vitro multiplication with TDZ and BAP from excised shoottips. African Journal of Biotechnology, 8 (21). art. no. 84C738E8553. pp. 5755-5761. ISSN 1684–5315 http://www.academicjournals.org/journal/AJB/article-abstract/84C738E8553 10.5897/AJB09.1080 |
| spellingShingle | Bidabadi, Siamak Shirani Mahdavi, Fatemeh Mahmood, Maziah Morphological abnormality among regenerated shoots of banana and plantain (Musa spp.) after in vitro multiplication with TDZ and BAP from excised shoottips |
| title | Morphological abnormality among regenerated shoots
of banana and plantain (Musa spp.) after in vitro
multiplication with TDZ and BAP from excised shoottips |
| title_full | Morphological abnormality among regenerated shoots
of banana and plantain (Musa spp.) after in vitro
multiplication with TDZ and BAP from excised shoottips |
| title_fullStr | Morphological abnormality among regenerated shoots
of banana and plantain (Musa spp.) after in vitro
multiplication with TDZ and BAP from excised shoottips |
| title_full_unstemmed | Morphological abnormality among regenerated shoots
of banana and plantain (Musa spp.) after in vitro
multiplication with TDZ and BAP from excised shoottips |
| title_short | Morphological abnormality among regenerated shoots
of banana and plantain (Musa spp.) after in vitro
multiplication with TDZ and BAP from excised shoottips |
| title_sort | morphological abnormality among regenerated shoots of banana and plantain musa spp after in vitro multiplication with tdz and bap from excised shoottips |
| url | http://psasir.upm.edu.my/id/eprint/15730/1/15730.pdf |
| work_keys_str_mv | AT bidabadisiamakshirani morphologicalabnormalityamongregeneratedshootsofbananaandplantainmusasppafterinvitromultiplicationwithtdzandbapfromexcisedshoottips AT mahdavifatemeh morphologicalabnormalityamongregeneratedshootsofbananaandplantainmusasppafterinvitromultiplicationwithtdzandbapfromexcisedshoottips AT mahmoodmaziah morphologicalabnormalityamongregeneratedshootsofbananaandplantainmusasppafterinvitromultiplicationwithtdzandbapfromexcisedshoottips |