Novel molecular analysis for characterization of staphylococcal cassette chromosome in a methicillin-resistant staphylococcus aureus isolated from Malaysian hospital.

Methicillin-resistant Staphylococcus aureus strains have appeared in countries worldwide and continue to be one of the most common hospital pathogens and it has become increasingly prevalent in community-acquired infections and provided strong evidence for the independent origins of health care-asso...

وصف كامل

التفاصيل البيبلوغرافية
المؤلفون الرئيسيون: Abdullah Al-Haj, Nagi Ahmed, E., Amghalia, Shamsudin, Mariana Nor, Abdullah, Rasedee, Mohamed, Rahmah, Sekawi, Zamberi
التنسيق: مقال
اللغة:English
English
منشور في: Medwell Publishing. 2009
الوصول للمادة أونلاين:http://psasir.upm.edu.my/id/eprint/15850/1/Novel%20molecular%20analysis%20for%20characterization%20of%20staphylococcal%20cassette%20chromosome%20in%20a%20methicillin.pdf
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author Abdullah Al-Haj, Nagi Ahmed
E., Amghalia
Shamsudin, Mariana Nor
Abdullah, Rasedee
Mohamed, Rahmah
Sekawi, Zamberi
author_facet Abdullah Al-Haj, Nagi Ahmed
E., Amghalia
Shamsudin, Mariana Nor
Abdullah, Rasedee
Mohamed, Rahmah
Sekawi, Zamberi
author_sort Abdullah Al-Haj, Nagi Ahmed
collection UPM
description Methicillin-resistant Staphylococcus aureus strains have appeared in countries worldwide and continue to be one of the most common hospital pathogens and it has become increasingly prevalent in community-acquired infections and provided strong evidence for the independent origins of health care-associated Methicillin-resistant Staphylococcus aureus and community-acquired. It has been shown that methicillin-susceptible S. aureus strains become MRSA strains by the acquisition of a staphylococcal cassette chromosome mec element carrying the mecA gene, which is responsible for methicillin resistance and has become essential for the characterization of Staphylococcus aureus clones in epidemiological studies. The objective of this study to identify the staphylococcal cassette chromosome mec types of methicillin-resistant Staphylococcus aureus isolated from different Malaysian Hospitals. PCR amplification and sequencing analysis were performed to determine the SCCmec type of MRSA. The present research successfully established molecular characteristics of local MRSA contribute as initial database of these isolates in order to fully understand the epidemiology, microbiology and pathophysiology of these infections.
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spelling upm.eprints-158502015-11-25T06:59:37Z http://psasir.upm.edu.my/id/eprint/15850/ Novel molecular analysis for characterization of staphylococcal cassette chromosome in a methicillin-resistant staphylococcus aureus isolated from Malaysian hospital. Abdullah Al-Haj, Nagi Ahmed E., Amghalia Shamsudin, Mariana Nor Abdullah, Rasedee Mohamed, Rahmah Sekawi, Zamberi Methicillin-resistant Staphylococcus aureus strains have appeared in countries worldwide and continue to be one of the most common hospital pathogens and it has become increasingly prevalent in community-acquired infections and provided strong evidence for the independent origins of health care-associated Methicillin-resistant Staphylococcus aureus and community-acquired. It has been shown that methicillin-susceptible S. aureus strains become MRSA strains by the acquisition of a staphylococcal cassette chromosome mec element carrying the mecA gene, which is responsible for methicillin resistance and has become essential for the characterization of Staphylococcus aureus clones in epidemiological studies. The objective of this study to identify the staphylococcal cassette chromosome mec types of methicillin-resistant Staphylococcus aureus isolated from different Malaysian Hospitals. PCR amplification and sequencing analysis were performed to determine the SCCmec type of MRSA. The present research successfully established molecular characteristics of local MRSA contribute as initial database of these isolates in order to fully understand the epidemiology, microbiology and pathophysiology of these infections. Medwell Publishing. 2009 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/15850/1/Novel%20molecular%20analysis%20for%20characterization%20of%20staphylococcal%20cassette%20chromosome%20in%20a%20methicillin.pdf Abdullah Al-Haj, Nagi Ahmed and E., Amghalia and Shamsudin, Mariana Nor and Abdullah, Rasedee and Mohamed, Rahmah and Sekawi, Zamberi (2009) Novel molecular analysis for characterization of staphylococcal cassette chromosome in a methicillin-resistant staphylococcus aureus isolated from Malaysian hospital. Research Journal of Biological Sciences, 4 (8). pp. 937-942. ISSN 1815-8846, ESSN: 1993-6087 http://www.medwelljournals.com/ rjbsci.2009.937.942 English
spellingShingle Abdullah Al-Haj, Nagi Ahmed
E., Amghalia
Shamsudin, Mariana Nor
Abdullah, Rasedee
Mohamed, Rahmah
Sekawi, Zamberi
Novel molecular analysis for characterization of staphylococcal cassette chromosome in a methicillin-resistant staphylococcus aureus isolated from Malaysian hospital.
title Novel molecular analysis for characterization of staphylococcal cassette chromosome in a methicillin-resistant staphylococcus aureus isolated from Malaysian hospital.
title_full Novel molecular analysis for characterization of staphylococcal cassette chromosome in a methicillin-resistant staphylococcus aureus isolated from Malaysian hospital.
title_fullStr Novel molecular analysis for characterization of staphylococcal cassette chromosome in a methicillin-resistant staphylococcus aureus isolated from Malaysian hospital.
title_full_unstemmed Novel molecular analysis for characterization of staphylococcal cassette chromosome in a methicillin-resistant staphylococcus aureus isolated from Malaysian hospital.
title_short Novel molecular analysis for characterization of staphylococcal cassette chromosome in a methicillin-resistant staphylococcus aureus isolated from Malaysian hospital.
title_sort novel molecular analysis for characterization of staphylococcal cassette chromosome in a methicillin resistant staphylococcus aureus isolated from malaysian hospital
url http://psasir.upm.edu.my/id/eprint/15850/1/Novel%20molecular%20analysis%20for%20characterization%20of%20staphylococcal%20cassette%20chromosome%20in%20a%20methicillin.pdf
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