Optimal transfection conditions and the safety profiles of dextran spermine/plasmid DNA as potential gene transfer vector to mouse airway.
The emergence of gene therapy offers a new paradigm to the field of molecular medicine. However, current viral and non-viral gene transfer vectors are not efficient and often restricted by doselimiting toxicity. Thus, generation of a new gene delivery vector, which is efficient and with good safet...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English English |
| Published: |
Malaysian Medical Association
2010
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| Online Access: | http://psasir.upm.edu.my/id/eprint/15952/1/Optimal%20transfection%20conditions%20and%20the%20safety%20profiles%20of%20dextran%20spermine.pdf |
| Summary: | The emergence of gene therapy offers a new paradigm to the field of molecular medicine. However, current viral and non-viral gene transfer vectors are not efficient and often restricted by doselimiting toxicity. Thus, generation of a new gene delivery vector, which is efficient and with good
safety profile is highly required. In this study, optimal
transfection conditions and safety profile a novel biodegradable cationic polymerdextran-spermine (DSPM) in mouse airways were ascertained. The highest level of gene expression in the lungs of BALB/c mice was detected at D-SPM to plasmid DNA (pDNA) weight ratio (w/w) of 16, with 13.5 µg
pDNA. No significant induction of pro-inflammatory cytokines in the broncho alveolar lavage fluids was observed, which implies no overt toxicity occurred in the mouse lungs. In short, these results demonstrate that D-SPM has moderate gene transfer efficiency but with acceptable safety profile in the mouse airways. |
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