Optimal transfection conditions and the safety profiles of dextran spermine/plasmid DNA as potential gene transfer vector to mouse airway.
The emergence of gene therapy offers a new paradigm to the field of molecular medicine. However, current viral and non-viral gene transfer vectors are not efficient and often restricted by doselimiting toxicity. Thus, generation of a new gene delivery vector, which is efficient and with good safet...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English English |
| Published: |
Malaysian Medical Association
2010
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| Online Access: | http://psasir.upm.edu.my/id/eprint/15952/1/Optimal%20transfection%20conditions%20and%20the%20safety%20profiles%20of%20dextran%20spermine.pdf |
| _version_ | 1796968955806482432 |
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| author | Rosli, Rozita Wendy-Yeo, W.Y. Veerakumarasivam, Abhinanyu Abdul Rahman, Sabariah Domb, A. J. Abdullah, Syahrilnizam |
| author_facet | Rosli, Rozita Wendy-Yeo, W.Y. Veerakumarasivam, Abhinanyu Abdul Rahman, Sabariah Domb, A. J. Abdullah, Syahrilnizam |
| author_sort | Rosli, Rozita |
| collection | UPM |
| description | The emergence of gene therapy offers a new paradigm to the field of molecular medicine. However, current viral and non-viral gene transfer vectors are not efficient and often restricted by doselimiting toxicity. Thus, generation of a new gene delivery vector, which is efficient and with good
safety profile is highly required. In this study, optimal
transfection conditions and safety profile a novel biodegradable cationic polymerdextran-spermine (DSPM) in mouse airways were ascertained. The highest level of gene expression in the lungs of BALB/c mice was detected at D-SPM to plasmid DNA (pDNA) weight ratio (w/w) of 16, with 13.5 µg
pDNA. No significant induction of pro-inflammatory cytokines in the broncho alveolar lavage fluids was observed, which implies no overt toxicity occurred in the mouse lungs. In short, these results demonstrate that D-SPM has moderate gene transfer efficiency but with acceptable safety profile in the mouse airways. |
| first_indexed | 2024-03-06T07:35:52Z |
| format | Article |
| id | upm.eprints-15952 |
| institution | Universiti Putra Malaysia |
| language | English English |
| last_indexed | 2024-03-06T07:35:52Z |
| publishDate | 2010 |
| publisher | Malaysian Medical Association |
| record_format | dspace |
| spelling | upm.eprints-159522015-11-02T02:07:20Z http://psasir.upm.edu.my/id/eprint/15952/ Optimal transfection conditions and the safety profiles of dextran spermine/plasmid DNA as potential gene transfer vector to mouse airway. Rosli, Rozita Wendy-Yeo, W.Y. Veerakumarasivam, Abhinanyu Abdul Rahman, Sabariah Domb, A. J. Abdullah, Syahrilnizam The emergence of gene therapy offers a new paradigm to the field of molecular medicine. However, current viral and non-viral gene transfer vectors are not efficient and often restricted by doselimiting toxicity. Thus, generation of a new gene delivery vector, which is efficient and with good safety profile is highly required. In this study, optimal transfection conditions and safety profile a novel biodegradable cationic polymerdextran-spermine (DSPM) in mouse airways were ascertained. The highest level of gene expression in the lungs of BALB/c mice was detected at D-SPM to plasmid DNA (pDNA) weight ratio (w/w) of 16, with 13.5 µg pDNA. No significant induction of pro-inflammatory cytokines in the broncho alveolar lavage fluids was observed, which implies no overt toxicity occurred in the mouse lungs. In short, these results demonstrate that D-SPM has moderate gene transfer efficiency but with acceptable safety profile in the mouse airways. Malaysian Medical Association 2010-10 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/15952/1/Optimal%20transfection%20conditions%20and%20the%20safety%20profiles%20of%20dextran%20spermine.pdf Rosli, Rozita and Wendy-Yeo, W.Y. and Veerakumarasivam, Abhinanyu and Abdul Rahman, Sabariah and Domb, A. J. and Abdullah, Syahrilnizam (2010) Optimal transfection conditions and the safety profiles of dextran spermine/plasmid DNA as potential gene transfer vector to mouse airway. Medical Journal of Malaysia, 65. pp. 116-117. ISSN 0300-5283 http://www.mma.org.my/ English |
| spellingShingle | Rosli, Rozita Wendy-Yeo, W.Y. Veerakumarasivam, Abhinanyu Abdul Rahman, Sabariah Domb, A. J. Abdullah, Syahrilnizam Optimal transfection conditions and the safety profiles of dextran spermine/plasmid DNA as potential gene transfer vector to mouse airway. |
| title | Optimal transfection conditions and the safety profiles of dextran spermine/plasmid DNA as potential gene transfer vector to mouse airway. |
| title_full | Optimal transfection conditions and the safety profiles of dextran spermine/plasmid DNA as potential gene transfer vector to mouse airway. |
| title_fullStr | Optimal transfection conditions and the safety profiles of dextran spermine/plasmid DNA as potential gene transfer vector to mouse airway. |
| title_full_unstemmed | Optimal transfection conditions and the safety profiles of dextran spermine/plasmid DNA as potential gene transfer vector to mouse airway. |
| title_short | Optimal transfection conditions and the safety profiles of dextran spermine/plasmid DNA as potential gene transfer vector to mouse airway. |
| title_sort | optimal transfection conditions and the safety profiles of dextran spermine plasmid dna as potential gene transfer vector to mouse airway |
| url | http://psasir.upm.edu.my/id/eprint/15952/1/Optimal%20transfection%20conditions%20and%20the%20safety%20profiles%20of%20dextran%20spermine.pdf |
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