Optimization of protein extraction from fish waste using Response Surface Methodology
The aim of present study was to optimize protein extraction from Fish Waste (FW), sardine (Sardina pilchardus) using enzymatic method. In this study, enzymatic method involved two combinations of enzymes which were alcalase and protamex. Response Surface Methodology (RSM) was used to study the effec...
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| Format: | Article |
| Language: | English English |
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Asian Network for Scientific Information
2009
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| Online Access: | http://psasir.upm.edu.my/id/eprint/15982/1/Optimization%20of%20protein%20extraction%20from%20fish%20waste%20using%20Response%20Surface%20Methodology.pdf |
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| author | Husin, Nurdiyana Mustapa Kamal, Siti Mazlina |
| author_facet | Husin, Nurdiyana Mustapa Kamal, Siti Mazlina |
| author_sort | Husin, Nurdiyana |
| collection | UPM |
| description | The aim of present study was to optimize protein extraction from Fish Waste (FW), sardine (Sardina pilchardus) using enzymatic method. In this study, enzymatic method involved two combinations of enzymes which were alcalase and protamex. Response Surface Methodology (RSM) was used to study the effect of independent variables, namely temperature (35-55°C), rotation speed (100-300 rpm), time (60-1440 min) and enzyme:substrate ratio (0.5-1.5) on protein extraction from FW. From RSM-generated model, the optimum conditions for extraction of protein from FW were identified to be at temperature 35°C in 1429 min reaction time, with rotation speed of 171 rpm and enzyme:substrate ratio of 1.50. At the optimum conditions, predicted protein yield in the extraction process was 80.75 mg mL-1. |
| first_indexed | 2024-03-06T07:35:58Z |
| format | Article |
| id | upm.eprints-15982 |
| institution | Universiti Putra Malaysia |
| language | English English |
| last_indexed | 2024-03-06T07:35:58Z |
| publishDate | 2009 |
| publisher | Asian Network for Scientific Information |
| record_format | dspace |
| spelling | upm.eprints-159822015-09-15T07:04:56Z http://psasir.upm.edu.my/id/eprint/15982/ Optimization of protein extraction from fish waste using Response Surface Methodology Husin, Nurdiyana Mustapa Kamal, Siti Mazlina The aim of present study was to optimize protein extraction from Fish Waste (FW), sardine (Sardina pilchardus) using enzymatic method. In this study, enzymatic method involved two combinations of enzymes which were alcalase and protamex. Response Surface Methodology (RSM) was used to study the effect of independent variables, namely temperature (35-55°C), rotation speed (100-300 rpm), time (60-1440 min) and enzyme:substrate ratio (0.5-1.5) on protein extraction from FW. From RSM-generated model, the optimum conditions for extraction of protein from FW were identified to be at temperature 35°C in 1429 min reaction time, with rotation speed of 171 rpm and enzyme:substrate ratio of 1.50. At the optimum conditions, predicted protein yield in the extraction process was 80.75 mg mL-1. Asian Network for Scientific Information 2009 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/15982/1/Optimization%20of%20protein%20extraction%20from%20fish%20waste%20using%20Response%20Surface%20Methodology.pdf Husin, Nurdiyana and Mustapa Kamal, Siti Mazlina (2009) Optimization of protein extraction from fish waste using Response Surface Methodology. Journal of Applied Sciences, 9 (17). pp. 3121-3125. ISSN 1812-5654; ESSN: 1812-5662 http://scialert.net/archivedetails.php?issn=1812-5654&issueno=121 10.3923/jas.2009.3121.3125 English |
| spellingShingle | Husin, Nurdiyana Mustapa Kamal, Siti Mazlina Optimization of protein extraction from fish waste using Response Surface Methodology |
| title | Optimization of protein extraction from fish waste using Response Surface Methodology |
| title_full | Optimization of protein extraction from fish waste using Response Surface Methodology |
| title_fullStr | Optimization of protein extraction from fish waste using Response Surface Methodology |
| title_full_unstemmed | Optimization of protein extraction from fish waste using Response Surface Methodology |
| title_short | Optimization of protein extraction from fish waste using Response Surface Methodology |
| title_sort | optimization of protein extraction from fish waste using response surface methodology |
| url | http://psasir.upm.edu.my/id/eprint/15982/1/Optimization%20of%20protein%20extraction%20from%20fish%20waste%20using%20Response%20Surface%20Methodology.pdf |
| work_keys_str_mv | AT husinnurdiyana optimizationofproteinextractionfromfishwasteusingresponsesurfacemethodology AT mustapakamalsitimazlina optimizationofproteinextractionfromfishwasteusingresponsesurfacemethodology |