Optimized enzymatic synthesis of levulinate ester in solvent-free system
Ethyl levulinate, produced through esterification of levulinic acid, is a ketoester with various applications. Synthesis of ethyl levulinate was carried out in solvent-free system using immobilized Candida antarctica lipase B (Novozym 435) as the biocatalyst for the reaction. Response surface method...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2010
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| Online Access: | http://psasir.upm.edu.my/id/eprint/15994/1/Optimized%20enzymatic%20synthesis%20of%20levulinate%20ester%20in%20solvent.pdf |
| _version_ | 1825945729601896448 |
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| author | Lee, Alice Chaibakhsha, Naz Abdul Rahman, Mohd Basyaruddin Basri, Mahiran Tejo, Bimo Ario |
| author_facet | Lee, Alice Chaibakhsha, Naz Abdul Rahman, Mohd Basyaruddin Basri, Mahiran Tejo, Bimo Ario |
| author_sort | Lee, Alice |
| collection | UPM |
| description | Ethyl levulinate, produced through esterification of levulinic acid, is a ketoester with various applications. Synthesis of ethyl levulinate was carried out in solvent-free system using immobilized Candida antarctica lipase B (Novozym 435) as the biocatalyst for the reaction. Response surface methodology (RSM) with a four-factor-five-level central composite rotatable design (CCRD) was employed to study and optimize the reaction conditions in the synthesis of levulinate ester. The effect of four main reaction parameters including time, temperature, ethanol/levulinic acid molar ratio and amount of enzyme on the synthesis of ester were analyzed. A quadratic polynomial model was fitted to the data with an R2 of 0.8993. Model validation experiments show good correspondence between actual and predicted values. A high conversion yield (96.2%) was obtained at the optimum conditions of 51.4 °C, 41.9 min, 292.3 mg enzyme amount and 1.1:1 alcohol:acid molar ratio. |
| first_indexed | 2024-03-06T07:36:00Z |
| format | Article |
| id | upm.eprints-15994 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T07:36:00Z |
| publishDate | 2010 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | upm.eprints-159942016-09-02T06:17:14Z http://psasir.upm.edu.my/id/eprint/15994/ Optimized enzymatic synthesis of levulinate ester in solvent-free system Lee, Alice Chaibakhsha, Naz Abdul Rahman, Mohd Basyaruddin Basri, Mahiran Tejo, Bimo Ario Ethyl levulinate, produced through esterification of levulinic acid, is a ketoester with various applications. Synthesis of ethyl levulinate was carried out in solvent-free system using immobilized Candida antarctica lipase B (Novozym 435) as the biocatalyst for the reaction. Response surface methodology (RSM) with a four-factor-five-level central composite rotatable design (CCRD) was employed to study and optimize the reaction conditions in the synthesis of levulinate ester. The effect of four main reaction parameters including time, temperature, ethanol/levulinic acid molar ratio and amount of enzyme on the synthesis of ester were analyzed. A quadratic polynomial model was fitted to the data with an R2 of 0.8993. Model validation experiments show good correspondence between actual and predicted values. A high conversion yield (96.2%) was obtained at the optimum conditions of 51.4 °C, 41.9 min, 292.3 mg enzyme amount and 1.1:1 alcohol:acid molar ratio. Elsevier 2010 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/15994/1/Optimized%20enzymatic%20synthesis%20of%20levulinate%20ester%20in%20solvent.pdf Lee, Alice and Chaibakhsha, Naz and Abdul Rahman, Mohd Basyaruddin and Basri, Mahiran and Tejo, Bimo Ario (2010) Optimized enzymatic synthesis of levulinate ester in solvent-free system. Industrial Crops and Products, 32 (3). pp. 246-251. ISSN 0926-6690; ESSN: 1872-633X 10.1016/j.indcrop.2010.04.022 |
| spellingShingle | Lee, Alice Chaibakhsha, Naz Abdul Rahman, Mohd Basyaruddin Basri, Mahiran Tejo, Bimo Ario Optimized enzymatic synthesis of levulinate ester in solvent-free system |
| title | Optimized enzymatic synthesis of levulinate ester in solvent-free system |
| title_full | Optimized enzymatic synthesis of levulinate ester in solvent-free system |
| title_fullStr | Optimized enzymatic synthesis of levulinate ester in solvent-free system |
| title_full_unstemmed | Optimized enzymatic synthesis of levulinate ester in solvent-free system |
| title_short | Optimized enzymatic synthesis of levulinate ester in solvent-free system |
| title_sort | optimized enzymatic synthesis of levulinate ester in solvent free system |
| url | http://psasir.upm.edu.my/id/eprint/15994/1/Optimized%20enzymatic%20synthesis%20of%20levulinate%20ester%20in%20solvent.pdf |
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