Optimizing conditions for methylmercury extraction from fish samples for GC analysis using response surface methodology.
Response surface methodology (RSM) was used to determine the optimum experimental conditions to extract methylmercury from fish samples for GC analysis. The influence of four variables - acid concentration (3-12 M), cysteine concentration (0.5-2% w/v), solvent volume (3-9 ml) and extraction time (10...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English English |
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Taylor & Francis
2009
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| Online Access: | http://psasir.upm.edu.my/id/eprint/15996/1/Optimizing%20conditions%20for%20methylmercury%20extraction%20from%20fish%20samples%20for%20GC%20analysis%20using%20response%20surface%20methodology.pdf |
| _version_ | 1825945729986723840 |
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| author | P., Hajeb Selamat, Jinap Abu Bakar, Fatimah Bakar, Jamilah |
| author_facet | P., Hajeb Selamat, Jinap Abu Bakar, Fatimah Bakar, Jamilah |
| author_sort | P., Hajeb |
| collection | UPM |
| description | Response surface methodology (RSM) was used to determine the optimum experimental conditions to extract methylmercury from fish samples for GC analysis. The influence of four variables - acid concentration (3-12 M), cysteine concentration (0.5-2% w/v), solvent volume (3-9 ml) and extraction time (10-30 min) - on recovery of methylmercury was evaluated. The detection limit for methylmercury analysis using a microelectron capture detector was 7 ng g-1 in fish samples. The mean recovery under optimum conditions was 94%. Experimental data were adequately fitted into a second-order polynomial model with multiple regression coefficients (r2) of 0.977. The four variables had a significant effect (p < 0.05) on the recovery of methylmercury from a reference material (BCR-463). Optimum conditions for methylmercury extraction were found using an acid concentration of 12.2 M, cysteine concentration of 2.4%, solvent volume of 1.5 ml and extraction time of 35 min. The validation of the developed method to analyze methylmercury in fish samples exhibited good agreement with mercury content in the samples. |
| first_indexed | 2024-03-06T07:36:00Z |
| format | Article |
| id | upm.eprints-15996 |
| institution | Universiti Putra Malaysia |
| language | English English |
| last_indexed | 2024-03-06T07:36:00Z |
| publishDate | 2009 |
| publisher | Taylor & Francis |
| record_format | dspace |
| spelling | upm.eprints-159962015-10-29T00:11:05Z http://psasir.upm.edu.my/id/eprint/15996/ Optimizing conditions for methylmercury extraction from fish samples for GC analysis using response surface methodology. P., Hajeb Selamat, Jinap Abu Bakar, Fatimah Bakar, Jamilah Response surface methodology (RSM) was used to determine the optimum experimental conditions to extract methylmercury from fish samples for GC analysis. The influence of four variables - acid concentration (3-12 M), cysteine concentration (0.5-2% w/v), solvent volume (3-9 ml) and extraction time (10-30 min) - on recovery of methylmercury was evaluated. The detection limit for methylmercury analysis using a microelectron capture detector was 7 ng g-1 in fish samples. The mean recovery under optimum conditions was 94%. Experimental data were adequately fitted into a second-order polynomial model with multiple regression coefficients (r2) of 0.977. The four variables had a significant effect (p < 0.05) on the recovery of methylmercury from a reference material (BCR-463). Optimum conditions for methylmercury extraction were found using an acid concentration of 12.2 M, cysteine concentration of 2.4%, solvent volume of 1.5 ml and extraction time of 35 min. The validation of the developed method to analyze methylmercury in fish samples exhibited good agreement with mercury content in the samples. Taylor & Francis 2009 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/15996/1/Optimizing%20conditions%20for%20methylmercury%20extraction%20from%20fish%20samples%20for%20GC%20analysis%20using%20response%20surface%20methodology.pdf P., Hajeb and Selamat, Jinap and Abu Bakar, Fatimah and Bakar, Jamilah (2009) Optimizing conditions for methylmercury extraction from fish samples for GC analysis using response surface methodology. Food Additives & Contaminants: Part A, 26 (6). pp. 829-838. ISSN 1944-0049; ESSN: 1944-0057 10.1080/02652030902751704 English |
| spellingShingle | P., Hajeb Selamat, Jinap Abu Bakar, Fatimah Bakar, Jamilah Optimizing conditions for methylmercury extraction from fish samples for GC analysis using response surface methodology. |
| title | Optimizing conditions for methylmercury extraction from fish samples for GC analysis using response surface methodology. |
| title_full | Optimizing conditions for methylmercury extraction from fish samples for GC analysis using response surface methodology. |
| title_fullStr | Optimizing conditions for methylmercury extraction from fish samples for GC analysis using response surface methodology. |
| title_full_unstemmed | Optimizing conditions for methylmercury extraction from fish samples for GC analysis using response surface methodology. |
| title_short | Optimizing conditions for methylmercury extraction from fish samples for GC analysis using response surface methodology. |
| title_sort | optimizing conditions for methylmercury extraction from fish samples for gc analysis using response surface methodology |
| url | http://psasir.upm.edu.my/id/eprint/15996/1/Optimizing%20conditions%20for%20methylmercury%20extraction%20from%20fish%20samples%20for%20GC%20analysis%20using%20response%20surface%20methodology.pdf |
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