Partition of plasmid DNA in polymer-salt aqueous two phase systems
The partition of plasmid DNA (pDNA) in polyethylene glycol (PEG)–phosphate aqueous two-phase systems (ATPS) is presented. A high molecular weight (HMW) and a low molecular weight (LMW) polymer, PEG-1450 and -300, were used in combination with di-potassium hydrogen phosphate. The experimental results...
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| Format: | Article |
| Language: | English English |
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Elsevier
2009
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| Online Access: | http://psasir.upm.edu.my/id/eprint/16053/1/Partition%20of%20plasmid%20DNA%20in%20polymer.pdf |
| _version_ | 1796968976455041024 |
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| author | Luechau, Frank Tau, Chuan Ling Lyddiatt, Andrew |
| author_facet | Luechau, Frank Tau, Chuan Ling Lyddiatt, Andrew |
| author_sort | Luechau, Frank |
| collection | UPM |
| description | The partition of plasmid DNA (pDNA) in polyethylene glycol (PEG)–phosphate aqueous two-phase systems (ATPS) is presented. A high molecular weight (HMW) and a low molecular weight (LMW) polymer, PEG-1450 and -300, were used in combination with di-potassium hydrogen phosphate. The experimental results demonstrated that the plasmid pTX0161 displays a varied partition behaviour in PEG–phosphate ATPS. In HMW PEG (PEG-1450–phosphate systems), pDNA partitioned to the bottom phase only. In LMW PEG (PEG-300–phosphate systems), pDNA partitioned to all of the phases with respect to the phase composition, system temperature and concentration of lysate used in the ATPS. In systems with volume ratios higher than one, pDNA was mainly recovered in the top phase. For volume ratios between 0.5 and 1, pDNA mainly partitioned to the interface. In systems with volume ratios below 0.5, most of the pDNA was recovered in the bottom phase. For temperatures between 4 and 25°C, the partition to the top phase decreased whereas partition to the interface steadily increased. At 25°C, over 80% of pDNA was recovered in the interface. The partition to the bottom phase increased steadily with increasing temperatures up to 40°C and the partition to the interface decreased. At 20°C, the recovery of pDNA in the interface gradually increased and reached a maximum at 60% (w/w) lysate with 80% recovery recorded. At 25°C, over 80% of pDNA was recovered in the interface from lysate concentrations greater than 35% (w/w). At 30°C, the top phase preference changed to an interface preference between 0 and 20% (w/w) lysate. |
| first_indexed | 2024-03-06T07:36:11Z |
| format | Article |
| id | upm.eprints-16053 |
| institution | Universiti Putra Malaysia |
| language | English English |
| last_indexed | 2024-03-06T07:36:11Z |
| publishDate | 2009 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | upm.eprints-160532015-10-05T06:05:22Z http://psasir.upm.edu.my/id/eprint/16053/ Partition of plasmid DNA in polymer-salt aqueous two phase systems Luechau, Frank Tau, Chuan Ling Lyddiatt, Andrew The partition of plasmid DNA (pDNA) in polyethylene glycol (PEG)–phosphate aqueous two-phase systems (ATPS) is presented. A high molecular weight (HMW) and a low molecular weight (LMW) polymer, PEG-1450 and -300, were used in combination with di-potassium hydrogen phosphate. The experimental results demonstrated that the plasmid pTX0161 displays a varied partition behaviour in PEG–phosphate ATPS. In HMW PEG (PEG-1450–phosphate systems), pDNA partitioned to the bottom phase only. In LMW PEG (PEG-300–phosphate systems), pDNA partitioned to all of the phases with respect to the phase composition, system temperature and concentration of lysate used in the ATPS. In systems with volume ratios higher than one, pDNA was mainly recovered in the top phase. For volume ratios between 0.5 and 1, pDNA mainly partitioned to the interface. In systems with volume ratios below 0.5, most of the pDNA was recovered in the bottom phase. For temperatures between 4 and 25°C, the partition to the top phase decreased whereas partition to the interface steadily increased. At 25°C, over 80% of pDNA was recovered in the interface. The partition to the bottom phase increased steadily with increasing temperatures up to 40°C and the partition to the interface decreased. At 20°C, the recovery of pDNA in the interface gradually increased and reached a maximum at 60% (w/w) lysate with 80% recovery recorded. At 25°C, over 80% of pDNA was recovered in the interface from lysate concentrations greater than 35% (w/w). At 30°C, the top phase preference changed to an interface preference between 0 and 20% (w/w) lysate. Elsevier 2009-04-20 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/16053/1/Partition%20of%20plasmid%20DNA%20in%20polymer.pdf Luechau, Frank and Tau, Chuan Ling and Lyddiatt, Andrew (2009) Partition of plasmid DNA in polymer-salt aqueous two phase systems. Separation and Purification Technology, 66 (2). pp. 397-404. ISSN 1383-5866 10.1016/j.seppur.2008.12.003 English |
| spellingShingle | Luechau, Frank Tau, Chuan Ling Lyddiatt, Andrew Partition of plasmid DNA in polymer-salt aqueous two phase systems |
| title | Partition of plasmid DNA in polymer-salt aqueous two phase systems |
| title_full | Partition of plasmid DNA in polymer-salt aqueous two phase systems |
| title_fullStr | Partition of plasmid DNA in polymer-salt aqueous two phase systems |
| title_full_unstemmed | Partition of plasmid DNA in polymer-salt aqueous two phase systems |
| title_short | Partition of plasmid DNA in polymer-salt aqueous two phase systems |
| title_sort | partition of plasmid dna in polymer salt aqueous two phase systems |
| url | http://psasir.upm.edu.my/id/eprint/16053/1/Partition%20of%20plasmid%20DNA%20in%20polymer.pdf |
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