Phenotypic and molecular identification of a novel thermophilic Anoxybacillus species: a lipase-producing bacterium isolated from a Malaysian hotspring.
Eleven lipase-producing thermophilic bacteria strains were recently isolated from Kuala Woh Hot Spring, in Peninsular Malaysia. These strains have been qualitatively screened using Rhodamine B-olive oil plate agar. All strains showed lipase activity in the range of 0.56–2.62 U/ml. Their thermostabil...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Springer Verlag
2009
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| _version_ | 1796969002664198144 |
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| author | Olusesan, Akanbi Taiwo Kamaruzaman, Liana Azura Abu Bakar, Fatimah N., Sheikh Abdul Hamid Radu, Son Saari, Nazamid |
| author_facet | Olusesan, Akanbi Taiwo Kamaruzaman, Liana Azura Abu Bakar, Fatimah N., Sheikh Abdul Hamid Radu, Son Saari, Nazamid |
| author_sort | Olusesan, Akanbi Taiwo |
| collection | UPM |
| description | Eleven lipase-producing thermophilic bacteria strains were recently isolated from Kuala Woh Hot Spring, in Peninsular Malaysia. These strains have been qualitatively screened using Rhodamine B-olive oil plate agar. All strains showed lipase activity in the range of 0.56–2.62 U/ml. Their thermostabilities were then determined by incubation at 80°C for 30 min. Results showed that strain KW 6 and KW 12 produced relatively thermostable lipases, which retained 62 and 54% of their original activity, respectively. They were identified based on their morphological characteristics, biochemical tests and the Biolog system. Strain KW 12 showed exceptionally unique characteristics (over KW 6) being able to grow in a broad range of pH and temperature. It was further identified using 16S rRNA partial sequence analysis and the result of 16S rRNA partial sequence analysis identified KW 12 as Anoxybacillus kamchatkensis.
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| first_indexed | 2024-03-06T07:36:34Z |
| format | Article |
| id | upm.eprints-16181 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T07:36:34Z |
| publishDate | 2009 |
| publisher | Springer Verlag |
| record_format | dspace |
| spelling | upm.eprints-161812012-08-27T04:14:49Z http://psasir.upm.edu.my/id/eprint/16181/ Phenotypic and molecular identification of a novel thermophilic Anoxybacillus species: a lipase-producing bacterium isolated from a Malaysian hotspring. Olusesan, Akanbi Taiwo Kamaruzaman, Liana Azura Abu Bakar, Fatimah N., Sheikh Abdul Hamid Radu, Son Saari, Nazamid Eleven lipase-producing thermophilic bacteria strains were recently isolated from Kuala Woh Hot Spring, in Peninsular Malaysia. These strains have been qualitatively screened using Rhodamine B-olive oil plate agar. All strains showed lipase activity in the range of 0.56–2.62 U/ml. Their thermostabilities were then determined by incubation at 80°C for 30 min. Results showed that strain KW 6 and KW 12 produced relatively thermostable lipases, which retained 62 and 54% of their original activity, respectively. They were identified based on their morphological characteristics, biochemical tests and the Biolog system. Strain KW 12 showed exceptionally unique characteristics (over KW 6) being able to grow in a broad range of pH and temperature. It was further identified using 16S rRNA partial sequence analysis and the result of 16S rRNA partial sequence analysis identified KW 12 as Anoxybacillus kamchatkensis. Springer Verlag 2009 Article PeerReviewed Olusesan, Akanbi Taiwo and Kamaruzaman, Liana Azura and Abu Bakar, Fatimah and N., Sheikh Abdul Hamid and Radu, Son and Saari, Nazamid (2009) Phenotypic and molecular identification of a novel thermophilic Anoxybacillus species: a lipase-producing bacterium isolated from a Malaysian hotspring. World Journal of Microbiology and Biotechnology, 25 (11). pp. 1981-1988. ISSN 0959-3993 Lipase. Thermophilic bacteria. Microorganisms - Detection. 10.1007/s11274-009-0097-0 English |
| spellingShingle | Lipase. Thermophilic bacteria. Microorganisms - Detection. Olusesan, Akanbi Taiwo Kamaruzaman, Liana Azura Abu Bakar, Fatimah N., Sheikh Abdul Hamid Radu, Son Saari, Nazamid Phenotypic and molecular identification of a novel thermophilic Anoxybacillus species: a lipase-producing bacterium isolated from a Malaysian hotspring. |
| title | Phenotypic and molecular identification of a novel thermophilic Anoxybacillus species: a lipase-producing bacterium isolated from a Malaysian hotspring. |
| title_full | Phenotypic and molecular identification of a novel thermophilic Anoxybacillus species: a lipase-producing bacterium isolated from a Malaysian hotspring. |
| title_fullStr | Phenotypic and molecular identification of a novel thermophilic Anoxybacillus species: a lipase-producing bacterium isolated from a Malaysian hotspring. |
| title_full_unstemmed | Phenotypic and molecular identification of a novel thermophilic Anoxybacillus species: a lipase-producing bacterium isolated from a Malaysian hotspring. |
| title_short | Phenotypic and molecular identification of a novel thermophilic Anoxybacillus species: a lipase-producing bacterium isolated from a Malaysian hotspring. |
| title_sort | phenotypic and molecular identification of a novel thermophilic anoxybacillus species a lipase producing bacterium isolated from a malaysian hotspring |
| topic | Lipase. Thermophilic bacteria. Microorganisms - Detection. |
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