Potential phytate-degrading enzyme producing bacteria isolated from Malaysian maize plantation
Phytases catalyze the hydrolysis of phosphomonoester bonds in phytate, thereby releasing lower forms of myo-inositol phosphates and inorganic phosphate. Phytase enzyme preparations have a wide range of applications in animal and human nutrition. The addition of phytate-degrading enzyme can improve t...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Academic Journals
2009
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| Online Access: | http://psasir.upm.edu.my/id/eprint/16266/1/16266.pdf |
| _version_ | 1796969020121939968 |
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| author | Meor Hussin, Anis Shobirin Abd. El Aziem, Farouk Greiner, Ralf |
| author_facet | Meor Hussin, Anis Shobirin Abd. El Aziem, Farouk Greiner, Ralf |
| author_sort | Meor Hussin, Anis Shobirin |
| collection | UPM |
| description | Phytases catalyze the hydrolysis of phosphomonoester bonds in phytate, thereby releasing lower forms of myo-inositol phosphates and inorganic phosphate. Phytase enzyme preparations have a wide range of applications in animal and human nutrition. The addition of phytate-degrading enzyme can improve the nutritional value of plant-based foods by enhancing protein digestibility and mineral availability through phytate hydrolysis during digestion in stomach or during food processing. 30 strains of potential phytate-degrading enzymes bacteria isolated from Malaysian maize plantation were cultivated in Luria Bertani (LB) and Luria Bertani + Rice Bran (LBRB) media for 5 days and were analyzed for phytase activity. The 6 strains with highest activity were chosen for species identification. Two set of broad-range 16S rRNA PCR primers were used for genotypic identification. ASUIA279 was the strain that had has the highest phytase activity in LBRB followed by ASUIA271, ASUIA138, ASUIA260, ASUIA243 and ASUIA30. The genotypic technique revealed Pantoea stewartii ASUIA271, Enterobacter sakazakiiASUIA279, Bacillus cereus ASUIA260, Bacillus subtilis ASUIA243, P. stewartiiASUIA138 and B. cereus ASUIA30. |
| first_indexed | 2024-03-06T07:36:55Z |
| format | Article |
| id | upm.eprints-16266 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T07:36:55Z |
| publishDate | 2009 |
| publisher | Academic Journals |
| record_format | dspace |
| spelling | upm.eprints-162662017-11-14T09:10:56Z http://psasir.upm.edu.my/id/eprint/16266/ Potential phytate-degrading enzyme producing bacteria isolated from Malaysian maize plantation Meor Hussin, Anis Shobirin Abd. El Aziem, Farouk Greiner, Ralf Phytases catalyze the hydrolysis of phosphomonoester bonds in phytate, thereby releasing lower forms of myo-inositol phosphates and inorganic phosphate. Phytase enzyme preparations have a wide range of applications in animal and human nutrition. The addition of phytate-degrading enzyme can improve the nutritional value of plant-based foods by enhancing protein digestibility and mineral availability through phytate hydrolysis during digestion in stomach or during food processing. 30 strains of potential phytate-degrading enzymes bacteria isolated from Malaysian maize plantation were cultivated in Luria Bertani (LB) and Luria Bertani + Rice Bran (LBRB) media for 5 days and were analyzed for phytase activity. The 6 strains with highest activity were chosen for species identification. Two set of broad-range 16S rRNA PCR primers were used for genotypic identification. ASUIA279 was the strain that had has the highest phytase activity in LBRB followed by ASUIA271, ASUIA138, ASUIA260, ASUIA243 and ASUIA30. The genotypic technique revealed Pantoea stewartii ASUIA271, Enterobacter sakazakiiASUIA279, Bacillus cereus ASUIA260, Bacillus subtilis ASUIA243, P. stewartiiASUIA138 and B. cereus ASUIA30. Academic Journals 2009 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/16266/1/16266.pdf Meor Hussin, Anis Shobirin and Abd. El Aziem, Farouk and Greiner, Ralf (2009) Potential phytate-degrading enzyme producing bacteria isolated from Malaysian maize plantation. African Journal of Biotechnology, 8 (15). art. no. E64FDFA6600. pp. 3540-3546. ISSN 1684–5315 http://www.academicjournals.org/journal/AJB/article-abstract/E64FDFA6600 10.5897/AJB09.309 |
| spellingShingle | Meor Hussin, Anis Shobirin Abd. El Aziem, Farouk Greiner, Ralf Potential phytate-degrading enzyme producing bacteria isolated from Malaysian maize plantation |
| title | Potential phytate-degrading enzyme producing bacteria isolated from Malaysian maize plantation |
| title_full | Potential phytate-degrading enzyme producing bacteria isolated from Malaysian maize plantation |
| title_fullStr | Potential phytate-degrading enzyme producing bacteria isolated from Malaysian maize plantation |
| title_full_unstemmed | Potential phytate-degrading enzyme producing bacteria isolated from Malaysian maize plantation |
| title_short | Potential phytate-degrading enzyme producing bacteria isolated from Malaysian maize plantation |
| title_sort | potential phytate degrading enzyme producing bacteria isolated from malaysian maize plantation |
| url | http://psasir.upm.edu.my/id/eprint/16266/1/16266.pdf |
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