Primary capture of high molecular weight nucleic acids using aqueous two-phase systems
The practical feasibility and generic applicability of the selective partition of plasmid DNA (pDNA) and RNA in polyethylene glycol 300 (PEG-300) and di-potassium hydrogen phosphate aqueous two-phase systems (ATPS) by addition of NaCl salt was investigated with partially disrupted Escherichia coli (...
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| Format: | Article |
| Language: | English |
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Elsevier
2009
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| Online Access: | http://psasir.upm.edu.my/id/eprint/16338/1/Primary%20capture%20of%20high%20molecular%20weight%20nucleic%20acids%20using%20aqueous%20two.pdf |
| _version_ | 1796969034949853184 |
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| author | Luechau, Frank Ling, Tau Chuan Lyddiatt, Andrew |
| author_facet | Luechau, Frank Ling, Tau Chuan Lyddiatt, Andrew |
| author_sort | Luechau, Frank |
| collection | UPM |
| description | The practical feasibility and generic applicability of the selective partition of plasmid DNA (pDNA) and RNA in polyethylene glycol 300 (PEG-300) and di-potassium hydrogen phosphate aqueous two-phase systems (ATPS) by addition of NaCl salt was investigated with partially disrupted Escherichia coli (E. coli) cell paste. The process yielded a relatively protein-free pDNA solution with 89.5% pDNA recovery while low molecular weight RNA was partially removed. The results gained here led the way to an alternative process options for the processing of pDNA. A reproducible process involving two extraction steps to recover pDNA from partially disrupted E. coli cell paste was developed and is presented here. |
| first_indexed | 2024-03-06T07:37:08Z |
| format | Article |
| id | upm.eprints-16338 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T07:37:08Z |
| publishDate | 2009 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | upm.eprints-163382015-10-05T05:53:36Z http://psasir.upm.edu.my/id/eprint/16338/ Primary capture of high molecular weight nucleic acids using aqueous two-phase systems Luechau, Frank Ling, Tau Chuan Lyddiatt, Andrew The practical feasibility and generic applicability of the selective partition of plasmid DNA (pDNA) and RNA in polyethylene glycol 300 (PEG-300) and di-potassium hydrogen phosphate aqueous two-phase systems (ATPS) by addition of NaCl salt was investigated with partially disrupted Escherichia coli (E. coli) cell paste. The process yielded a relatively protein-free pDNA solution with 89.5% pDNA recovery while low molecular weight RNA was partially removed. The results gained here led the way to an alternative process options for the processing of pDNA. A reproducible process involving two extraction steps to recover pDNA from partially disrupted E. coli cell paste was developed and is presented here. Elsevier 2009-04-07 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/16338/1/Primary%20capture%20of%20high%20molecular%20weight%20nucleic%20acids%20using%20aqueous%20two.pdf Luechau, Frank and Ling, Tau Chuan and Lyddiatt, Andrew (2009) Primary capture of high molecular weight nucleic acids using aqueous two-phase systems. Separation and Purification Technology, 66 (1). pp. 202-207. ISSN 1383-5866; ESSN: 1873-3794 10.1016/j.seppur.2008.12.008 |
| spellingShingle | Luechau, Frank Ling, Tau Chuan Lyddiatt, Andrew Primary capture of high molecular weight nucleic acids using aqueous two-phase systems |
| title | Primary capture of high molecular weight nucleic acids using aqueous two-phase systems |
| title_full | Primary capture of high molecular weight nucleic acids using aqueous two-phase systems |
| title_fullStr | Primary capture of high molecular weight nucleic acids using aqueous two-phase systems |
| title_full_unstemmed | Primary capture of high molecular weight nucleic acids using aqueous two-phase systems |
| title_short | Primary capture of high molecular weight nucleic acids using aqueous two-phase systems |
| title_sort | primary capture of high molecular weight nucleic acids using aqueous two phase systems |
| url | http://psasir.upm.edu.my/id/eprint/16338/1/Primary%20capture%20of%20high%20molecular%20weight%20nucleic%20acids%20using%20aqueous%20two.pdf |
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