Profile of enzymes relevant to solvent production during direct fermentation of sago starch by C. saccharobutylicum at different pH control strategies.
The profile of enzymes relevant to solvent production during direct fermentation of sago starch by clostidium saccharobutylicum P262 in a 2 L stirred tank fermenter was determined utilizing different pH control strategies. During fermentation without pH control (initial pH of 6), the specific activi...
| Príomhchruthaitheoirí: | , , |
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| Formáid: | Alt |
| Teanga: | English English |
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The Korean Society for Biotechnology and Bioengineering.
2008
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| Ábhair: | |
| Rochtain ar líne: | http://psasir.upm.edu.my/id/eprint/16365/1/Profile%20of%20enzymes%20relevant%20to%20solvent%20production%20during%20direct%20fermentation%20of%20sago%20starch%20by%20C.pdf |
| _version_ | 1825945802028089344 |
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| author | Md Salleh, Madihah Liew, Shiau Tsuey Ariff, Arbakariya |
| author_facet | Md Salleh, Madihah Liew, Shiau Tsuey Ariff, Arbakariya |
| author_sort | Md Salleh, Madihah |
| collection | UPM |
| description | The profile of enzymes relevant to solvent production during direct fermentation of sago starch by clostidium saccharobutylicum P262 in a 2 L stirred tank fermenter was determined utilizing different pH control strategies. During fermentation without pH control (initial pH of 6), the specific activity of crotonase, thiolase, and β-hydroxybutyryl-CoA dehydrogenase increased proportionally with solvent production. The highest crotonase (3,450.7 kat) and phosphotransbutyrylase activity(1,475.6 kat) was observed in fermentation where pH was maintained at 5 during the acidogenic phase and corresponded to a fairly high acid accumulation but low solvent production. During fermentation with a controlled pH of 5.25 during the solventogenic phase, the highest thiolase specific activity (255.7 kat) was obtained and corresponded to the highest production of acetone. On the other hand, the highest specific activities of crotonase, β-hydroxybutyryl-CoA dehydrogenase, and phosphotransbutyrylase were observed at pH 5.5 and corresponded to the highest production of ethanol and butanol. Butyryl-CoA dehydrogenase had no significance role in solvent fermentation. These results suggested that pH control strategies were important for improvement of solvent production during direct fermentation of sago starch by C.saccharobutylicum. ©
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| first_indexed | 2024-03-06T07:37:12Z |
| format | Article |
| id | upm.eprints-16365 |
| institution | Universiti Putra Malaysia |
| language | English English |
| last_indexed | 2024-03-06T07:37:12Z |
| publishDate | 2008 |
| publisher | The Korean Society for Biotechnology and Bioengineering. |
| record_format | dspace |
| spelling | upm.eprints-163652015-10-26T00:30:51Z http://psasir.upm.edu.my/id/eprint/16365/ Profile of enzymes relevant to solvent production during direct fermentation of sago starch by C. saccharobutylicum at different pH control strategies. Md Salleh, Madihah Liew, Shiau Tsuey Ariff, Arbakariya The profile of enzymes relevant to solvent production during direct fermentation of sago starch by clostidium saccharobutylicum P262 in a 2 L stirred tank fermenter was determined utilizing different pH control strategies. During fermentation without pH control (initial pH of 6), the specific activity of crotonase, thiolase, and β-hydroxybutyryl-CoA dehydrogenase increased proportionally with solvent production. The highest crotonase (3,450.7 kat) and phosphotransbutyrylase activity(1,475.6 kat) was observed in fermentation where pH was maintained at 5 during the acidogenic phase and corresponded to a fairly high acid accumulation but low solvent production. During fermentation with a controlled pH of 5.25 during the solventogenic phase, the highest thiolase specific activity (255.7 kat) was obtained and corresponded to the highest production of acetone. On the other hand, the highest specific activities of crotonase, β-hydroxybutyryl-CoA dehydrogenase, and phosphotransbutyrylase were observed at pH 5.5 and corresponded to the highest production of ethanol and butanol. Butyryl-CoA dehydrogenase had no significance role in solvent fermentation. These results suggested that pH control strategies were important for improvement of solvent production during direct fermentation of sago starch by C.saccharobutylicum. © KSBB The Korean Society for Biotechnology and Bioengineering. 2008 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/16365/1/Profile%20of%20enzymes%20relevant%20to%20solvent%20production%20during%20direct%20fermentation%20of%20sago%20starch%20by%20C.pdf Md Salleh, Madihah and Liew, Shiau Tsuey and Ariff, Arbakariya (2008) Profile of enzymes relevant to solvent production during direct fermentation of sago starch by C. saccharobutylicum at different pH control strategies. Biotechnology and Bioprocess Engineering, 13 (1). pp. 33-39. ISSN 1976-3816 Sago. Enzymes - Biotechnology. Sago starch. DOI/10.1007/s12257-007-0153-2 English |
| spellingShingle | Sago. Enzymes - Biotechnology. Sago starch. Md Salleh, Madihah Liew, Shiau Tsuey Ariff, Arbakariya Profile of enzymes relevant to solvent production during direct fermentation of sago starch by C. saccharobutylicum at different pH control strategies. |
| title | Profile of enzymes relevant to solvent production during direct fermentation of sago starch by C. saccharobutylicum at different pH control strategies. |
| title_full | Profile of enzymes relevant to solvent production during direct fermentation of sago starch by C. saccharobutylicum at different pH control strategies. |
| title_fullStr | Profile of enzymes relevant to solvent production during direct fermentation of sago starch by C. saccharobutylicum at different pH control strategies. |
| title_full_unstemmed | Profile of enzymes relevant to solvent production during direct fermentation of sago starch by C. saccharobutylicum at different pH control strategies. |
| title_short | Profile of enzymes relevant to solvent production during direct fermentation of sago starch by C. saccharobutylicum at different pH control strategies. |
| title_sort | profile of enzymes relevant to solvent production during direct fermentation of sago starch by c saccharobutylicum at different ph control strategies |
| topic | Sago. Enzymes - Biotechnology. Sago starch. |
| url | http://psasir.upm.edu.my/id/eprint/16365/1/Profile%20of%20enzymes%20relevant%20to%20solvent%20production%20during%20direct%20fermentation%20of%20sago%20starch%20by%20C.pdf |
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