Protein patterning by UV-induced photodegradation of poly(oligo(ethylene glycol) methacrylate) brushes
The UV photodegradation of protein-resistant poly(oligo(ethylene glycol) methacrylate) (POEGMA) bottle-brush films, grown on silicon oxide by surface-initiated atom radical transfer polymerization, was studied using X-ray photoelectron spectroscopy (XPS) and atomic force microscopy (AFM). Exposure t...
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| Format: | Article |
| Language: | English English |
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American Chemical Society
2010
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| Online Access: | http://psasir.upm.edu.my/id/eprint/16381/1/Protein%20patterning%20by%20UV.pdf |
| _version_ | 1825945805149700096 |
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| author | Alang Ahmad, Shahrul Hucknall, Agnus Chilkoti, Ashutosh Leggett, Graham J. |
| author_facet | Alang Ahmad, Shahrul Hucknall, Agnus Chilkoti, Ashutosh Leggett, Graham J. |
| author_sort | Alang Ahmad, Shahrul |
| collection | UPM |
| description | The UV photodegradation of protein-resistant poly(oligo(ethylene glycol) methacrylate) (POEGMA) bottle-brush films, grown on silicon oxide by surface-initiated atom radical transfer polymerization, was studied using X-ray photoelectron spectroscopy (XPS) and atomic force microscopy (AFM). Exposure to light with a wavelength of 244 nm caused a loss of polyether units from the brush structure and the creation of aldehyde groups that could be derivatized with amines. An increase was measured in the coefficient of friction of the photodegraded polymer brush compared to the native brush, attributed to the creation of a heterogeneous surface film, leading to increased energy dissipation through film deformation and the creation of new polar functional groups at the surface. Exposure of the films through a photomask yielded sharp, well-defined patterns. Analysis of topographical images showed that physical removal of material occurred during exposure, at a rate of 1.35 nm J−1 cm2. Using fluorescence microscopy, the adsorption of labeled proteins onto the exposed surfaces was studied. It was found that protein strongly adsorbed to exposed areas, while the masked regions retained their protein resistance. Exposure of the film to UV light from a scanning near-field optical microscope yielded submicrometer-scale patterns. These data indicate that a simple, rapid, one-step photoconversion of the poly(OEGMA) brush occurs that transforms it from a highly protein-resistant material to one that adsorbs protein and can covalently bind amine-containing molecules and that this photoconversion can be spatially addressed with high spatial resolution. |
| first_indexed | 2024-03-06T07:37:15Z |
| format | Article |
| id | upm.eprints-16381 |
| institution | Universiti Putra Malaysia |
| language | English English |
| last_indexed | 2024-03-06T07:37:15Z |
| publishDate | 2010 |
| publisher | American Chemical Society |
| record_format | dspace |
| spelling | upm.eprints-163812015-11-03T06:18:10Z http://psasir.upm.edu.my/id/eprint/16381/ Protein patterning by UV-induced photodegradation of poly(oligo(ethylene glycol) methacrylate) brushes Alang Ahmad, Shahrul Hucknall, Agnus Chilkoti, Ashutosh Leggett, Graham J. The UV photodegradation of protein-resistant poly(oligo(ethylene glycol) methacrylate) (POEGMA) bottle-brush films, grown on silicon oxide by surface-initiated atom radical transfer polymerization, was studied using X-ray photoelectron spectroscopy (XPS) and atomic force microscopy (AFM). Exposure to light with a wavelength of 244 nm caused a loss of polyether units from the brush structure and the creation of aldehyde groups that could be derivatized with amines. An increase was measured in the coefficient of friction of the photodegraded polymer brush compared to the native brush, attributed to the creation of a heterogeneous surface film, leading to increased energy dissipation through film deformation and the creation of new polar functional groups at the surface. Exposure of the films through a photomask yielded sharp, well-defined patterns. Analysis of topographical images showed that physical removal of material occurred during exposure, at a rate of 1.35 nm J−1 cm2. Using fluorescence microscopy, the adsorption of labeled proteins onto the exposed surfaces was studied. It was found that protein strongly adsorbed to exposed areas, while the masked regions retained their protein resistance. Exposure of the film to UV light from a scanning near-field optical microscope yielded submicrometer-scale patterns. These data indicate that a simple, rapid, one-step photoconversion of the poly(OEGMA) brush occurs that transforms it from a highly protein-resistant material to one that adsorbs protein and can covalently bind amine-containing molecules and that this photoconversion can be spatially addressed with high spatial resolution. American Chemical Society 2010 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/16381/1/Protein%20patterning%20by%20UV.pdf Alang Ahmad, Shahrul and Hucknall, Agnus and Chilkoti, Ashutosh and Leggett, Graham J. (2010) Protein patterning by UV-induced photodegradation of poly(oligo(ethylene glycol) methacrylate) brushes. Langmuir, 26 (12). pp. 9937-9942. ISSN 0743-7463 Photodegradation Surfaces (Technology)—Analysis 10.1021/la100438d English |
| spellingShingle | Photodegradation Surfaces (Technology)—Analysis Alang Ahmad, Shahrul Hucknall, Agnus Chilkoti, Ashutosh Leggett, Graham J. Protein patterning by UV-induced photodegradation of poly(oligo(ethylene glycol) methacrylate) brushes |
| title | Protein patterning by UV-induced photodegradation of poly(oligo(ethylene glycol) methacrylate) brushes |
| title_full | Protein patterning by UV-induced photodegradation of poly(oligo(ethylene glycol) methacrylate) brushes |
| title_fullStr | Protein patterning by UV-induced photodegradation of poly(oligo(ethylene glycol) methacrylate) brushes |
| title_full_unstemmed | Protein patterning by UV-induced photodegradation of poly(oligo(ethylene glycol) methacrylate) brushes |
| title_short | Protein patterning by UV-induced photodegradation of poly(oligo(ethylene glycol) methacrylate) brushes |
| title_sort | protein patterning by uv induced photodegradation of poly oligo ethylene glycol methacrylate brushes |
| topic | Photodegradation Surfaces (Technology)—Analysis |
| url | http://psasir.upm.edu.my/id/eprint/16381/1/Protein%20patterning%20by%20UV.pdf |
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