Purification of bacteriophage M13 by anion exchange chromatography
M13 is a non-lytic filamentous bacteriophage (phage). It has been used widely in phage display technology for displaying foreign peptides, and also for studying macromolecule structures and interactions. Traditionally, this phage has been purified by cesium chloride (CsCl) density gradient ultracent...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2010
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| Online Access: | http://psasir.upm.edu.my/id/eprint/16393/1/Purification%20of%20bacteriophage%20M13%20by%20anion%20exchange%20chromatography.pdf |
| _version_ | 1796969046112993280 |
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| author | Monjezi, Razieh Tey, Beng Ti Sieo, Chin Chin Tan, Wen Siang |
| author_facet | Monjezi, Razieh Tey, Beng Ti Sieo, Chin Chin Tan, Wen Siang |
| author_sort | Monjezi, Razieh |
| collection | UPM |
| description | M13 is a non-lytic filamentous bacteriophage (phage). It has been used widely in phage display technology for displaying foreign peptides, and also for studying macromolecule structures and interactions. Traditionally, this phage has been purified by cesium chloride (CsCl) density gradient ultracentrifugation which is highly laborious and time consuming. In the present study, a simple, rapid and efficient method for the purification of M13 based on anion exchange chromatography was established. A pre-packed SepFast™ Super Q column connected to a fast protein liquid chromatography (FPLC) system was employed to capture released phages in clarified Escherichia coli fermented broth. An average yield of 74% was obtained from a packed bed mode elution using citrate buffer (pH 4), containing 1.5 M NaCl at 1 ml/min flow rate. The purification process was shortened substantially to less than 2 h from 18 h in the conventional ultracentrifugation method. SDS-PAGE revealed that the purity of particles was comparable to that of CsCl gradient density ultracentrifugation method. Plaque forming assay showed that the purified phages were still infectious. |
| first_indexed | 2024-03-06T07:37:17Z |
| format | Article |
| id | upm.eprints-16393 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T07:37:17Z |
| publishDate | 2010 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | upm.eprints-163932015-10-20T08:17:52Z http://psasir.upm.edu.my/id/eprint/16393/ Purification of bacteriophage M13 by anion exchange chromatography Monjezi, Razieh Tey, Beng Ti Sieo, Chin Chin Tan, Wen Siang M13 is a non-lytic filamentous bacteriophage (phage). It has been used widely in phage display technology for displaying foreign peptides, and also for studying macromolecule structures and interactions. Traditionally, this phage has been purified by cesium chloride (CsCl) density gradient ultracentrifugation which is highly laborious and time consuming. In the present study, a simple, rapid and efficient method for the purification of M13 based on anion exchange chromatography was established. A pre-packed SepFast™ Super Q column connected to a fast protein liquid chromatography (FPLC) system was employed to capture released phages in clarified Escherichia coli fermented broth. An average yield of 74% was obtained from a packed bed mode elution using citrate buffer (pH 4), containing 1.5 M NaCl at 1 ml/min flow rate. The purification process was shortened substantially to less than 2 h from 18 h in the conventional ultracentrifugation method. SDS-PAGE revealed that the purity of particles was comparable to that of CsCl gradient density ultracentrifugation method. Plaque forming assay showed that the purified phages were still infectious. Elsevier 2010-07-01 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/16393/1/Purification%20of%20bacteriophage%20M13%20by%20anion%20exchange%20chromatography.pdf Monjezi, Razieh and Tey, Beng Ti and Sieo, Chin Chin and Tan, Wen Siang (2010) Purification of bacteriophage M13 by anion exchange chromatography. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, 878 (21). pp. 1855-1859. ISSN 1570-0232 10.1016/j.jchromb.2010.05.028 |
| spellingShingle | Monjezi, Razieh Tey, Beng Ti Sieo, Chin Chin Tan, Wen Siang Purification of bacteriophage M13 by anion exchange chromatography |
| title | Purification of bacteriophage M13 by anion exchange chromatography |
| title_full | Purification of bacteriophage M13 by anion exchange chromatography |
| title_fullStr | Purification of bacteriophage M13 by anion exchange chromatography |
| title_full_unstemmed | Purification of bacteriophage M13 by anion exchange chromatography |
| title_short | Purification of bacteriophage M13 by anion exchange chromatography |
| title_sort | purification of bacteriophage m13 by anion exchange chromatography |
| url | http://psasir.upm.edu.my/id/eprint/16393/1/Purification%20of%20bacteriophage%20M13%20by%20anion%20exchange%20chromatography.pdf |
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