Putative, phenylacetaldehyde synthase transcript of vanda mimi palmer: sequence and expression analysis.
Vanda Mimi Palmer is a fragrant-orchid of which has won several international awards for its fragrance emission characteristic. A partial sequence encoding putative phenylacetaldehyde synthase (VMPPAAS) transcript which might be involved in the fragrance biosynthesis of this orchid was previously id...
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| 语言: | English |
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Asian Network for Scientific Information
2010
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| 在线阅读: | http://psasir.upm.edu.my/id/eprint/16399/1/Putative.pdf |
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| author | Mohd Hairul, Ab. Rahim Chan, Wai Sun Parameswari, Namasivayam Gwendoline, Ee Cheng Lian Abdullah, Janna Ong |
| author_facet | Mohd Hairul, Ab. Rahim Chan, Wai Sun Parameswari, Namasivayam Gwendoline, Ee Cheng Lian Abdullah, Janna Ong |
| author_sort | Mohd Hairul, Ab. Rahim |
| collection | UPM |
| description | Vanda Mimi Palmer is a fragrant-orchid of which has won several international awards for its fragrance emission characteristic. A partial sequence encoding putative phenylacetaldehyde synthase (VMPPAAS) transcript which might be involved in the fragrance biosynthesis of this orchid was previously identified in the floral Expressed Sequence Tags (ESTs) of this orchid. The aims of this study were to isolate full-length cDNA of VMPPAAS and followed by molecular characterization. The full-length cDNA was isolated by SMART RACE PCR amplification while the expression analysis was carried out using real-time RT-PCR for different tissues, different flower developmental stages and different time points in a 24 h cycle. The VMPPAAS transcript encodes for a polypeptide of 508 amino acid residues consisting of 1524 bp Open Reading Frame (ORF) flanked by 92 bp of 5' Untranslated Region (UTR) and 93 bp of 3' UTR including a poly-A tail. It has a conserved motif for pyridoxal phosphate attachment site and a VHVDAAY motif, which are also shared by other plant decarboxylase proteins such as tyrosine decarboxylases, tryptophan decarboxylases and phenylacetaldehyde synthase. Expression analysis of VMPPAAS transcript by real-time RT-PCR showed an up-regulated expression in floral tissues especially in sepal and petal and down-regulated in vegetative tissues. The expression was shown to be developmentally regulated in the flower life-cycle as well as temporally regulated in a 24 h cycle. |
| first_indexed | 2024-03-06T07:37:18Z |
| format | Article |
| id | upm.eprints-16399 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T07:37:18Z |
| publishDate | 2010 |
| publisher | Asian Network for Scientific Information |
| record_format | dspace |
| spelling | upm.eprints-163992015-10-21T07:36:37Z http://psasir.upm.edu.my/id/eprint/16399/ Putative, phenylacetaldehyde synthase transcript of vanda mimi palmer: sequence and expression analysis. Mohd Hairul, Ab. Rahim Chan, Wai Sun Parameswari, Namasivayam Gwendoline, Ee Cheng Lian Abdullah, Janna Ong Vanda Mimi Palmer is a fragrant-orchid of which has won several international awards for its fragrance emission characteristic. A partial sequence encoding putative phenylacetaldehyde synthase (VMPPAAS) transcript which might be involved in the fragrance biosynthesis of this orchid was previously identified in the floral Expressed Sequence Tags (ESTs) of this orchid. The aims of this study were to isolate full-length cDNA of VMPPAAS and followed by molecular characterization. The full-length cDNA was isolated by SMART RACE PCR amplification while the expression analysis was carried out using real-time RT-PCR for different tissues, different flower developmental stages and different time points in a 24 h cycle. The VMPPAAS transcript encodes for a polypeptide of 508 amino acid residues consisting of 1524 bp Open Reading Frame (ORF) flanked by 92 bp of 5' Untranslated Region (UTR) and 93 bp of 3' UTR including a poly-A tail. It has a conserved motif for pyridoxal phosphate attachment site and a VHVDAAY motif, which are also shared by other plant decarboxylase proteins such as tyrosine decarboxylases, tryptophan decarboxylases and phenylacetaldehyde synthase. Expression analysis of VMPPAAS transcript by real-time RT-PCR showed an up-regulated expression in floral tissues especially in sepal and petal and down-regulated in vegetative tissues. The expression was shown to be developmentally regulated in the flower life-cycle as well as temporally regulated in a 24 h cycle. Asian Network for Scientific Information 2010 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/16399/1/Putative.pdf Mohd Hairul, Ab. Rahim and Chan, Wai Sun and Parameswari, Namasivayam and Gwendoline, Ee Cheng Lian and Abdullah, Janna Ong (2010) Putative, phenylacetaldehyde synthase transcript of vanda mimi palmer: sequence and expression analysis. International Journal of Botany, 6 (4). pp. 424-432. ISSN 1811-9700 Orchidaceae (Orchid) Flowers - Odor Aromatic plants - Analysis DOI: 10.3923/ijb.2010.424.432 |
| spellingShingle | Orchidaceae (Orchid) Flowers - Odor Aromatic plants - Analysis Mohd Hairul, Ab. Rahim Chan, Wai Sun Parameswari, Namasivayam Gwendoline, Ee Cheng Lian Abdullah, Janna Ong Putative, phenylacetaldehyde synthase transcript of vanda mimi palmer: sequence and expression analysis. |
| title | Putative, phenylacetaldehyde synthase transcript of vanda mimi palmer: sequence and expression analysis. |
| title_full | Putative, phenylacetaldehyde synthase transcript of vanda mimi palmer: sequence and expression analysis. |
| title_fullStr | Putative, phenylacetaldehyde synthase transcript of vanda mimi palmer: sequence and expression analysis. |
| title_full_unstemmed | Putative, phenylacetaldehyde synthase transcript of vanda mimi palmer: sequence and expression analysis. |
| title_short | Putative, phenylacetaldehyde synthase transcript of vanda mimi palmer: sequence and expression analysis. |
| title_sort | putative phenylacetaldehyde synthase transcript of vanda mimi palmer sequence and expression analysis |
| topic | Orchidaceae (Orchid) Flowers - Odor Aromatic plants - Analysis |
| url | http://psasir.upm.edu.my/id/eprint/16399/1/Putative.pdf |
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