Recombinant hepatitis B virus core particles: association, dissociation and encapsidation of green fluorescent protein.
The recombinant hepatitis B virus (HBV) core antigen (HBcAg) expressed in Escherichia coli self-assembles into icosahedral capsids of about 35 nm which can be exploited as gene or drug delivery vehicles. The association and dissociation properties of the C-terminally truncated HBcAg with urea and gu...
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| Format: | Article |
| Language: | English English |
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Elsevier
2008
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| Online Access: | http://psasir.upm.edu.my/id/eprint/16448/1/Recombinant%20hepatitis%20B%20virus%20core%20particles.pdf |
| _version_ | 1825945818131070976 |
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| author | Lee, Khai Wooi Tan, Wen Siang |
| author_facet | Lee, Khai Wooi Tan, Wen Siang |
| author_sort | Lee, Khai Wooi |
| collection | UPM |
| description | The recombinant hepatitis B virus (HBV) core antigen (HBcAg) expressed in Escherichia coli self-assembles into icosahedral capsids of about 35 nm which can be exploited as gene or drug delivery vehicles. The association and dissociation properties of the C-terminally truncated HBcAg with urea and guanidine hydrochloride (GdnHCl) were studied. Transmission electron microscopy (TEM) revealed that the dissociated HBcAg was able to re-associate into particles when the applied denaturing agents were physically removed. In order to evaluate the potential of the particles in capturing molecules, purified green fluorescent protein (GFP) was applied to the dissociated HBcAg for encapsidation. The HBcAg particles harbouring the GFP molecules were purified using sucrose density gradient ultracentrifugation and analysed using native agarose gel electrophoresis and TEM. A method for the encapsidation of GFP in HBcAg particles which has the potential to capture drugs or nucleic acids was established. |
| first_indexed | 2024-03-06T07:37:27Z |
| format | Article |
| id | upm.eprints-16448 |
| institution | Universiti Putra Malaysia |
| language | English English |
| last_indexed | 2024-03-06T07:37:27Z |
| publishDate | 2008 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | upm.eprints-164482015-10-05T08:28:29Z http://psasir.upm.edu.my/id/eprint/16448/ Recombinant hepatitis B virus core particles: association, dissociation and encapsidation of green fluorescent protein. Lee, Khai Wooi Tan, Wen Siang The recombinant hepatitis B virus (HBV) core antigen (HBcAg) expressed in Escherichia coli self-assembles into icosahedral capsids of about 35 nm which can be exploited as gene or drug delivery vehicles. The association and dissociation properties of the C-terminally truncated HBcAg with urea and guanidine hydrochloride (GdnHCl) were studied. Transmission electron microscopy (TEM) revealed that the dissociated HBcAg was able to re-associate into particles when the applied denaturing agents were physically removed. In order to evaluate the potential of the particles in capturing molecules, purified green fluorescent protein (GFP) was applied to the dissociated HBcAg for encapsidation. The HBcAg particles harbouring the GFP molecules were purified using sucrose density gradient ultracentrifugation and analysed using native agarose gel electrophoresis and TEM. A method for the encapsidation of GFP in HBcAg particles which has the potential to capture drugs or nucleic acids was established. Elsevier 2008 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/16448/1/Recombinant%20hepatitis%20B%20virus%20core%20particles.pdf Lee, Khai Wooi and Tan, Wen Siang (2008) Recombinant hepatitis B virus core particles: association, dissociation and encapsidation of green fluorescent protein. Journal of Virological Methods, 151 (2). pp. 172-180. ISSN 0166-0934 Hepatitis B virus. Hepatitis associated antigen. Green fluorescent protein. doi:10.1016/j.jviromet.2008.05.025 English |
| spellingShingle | Hepatitis B virus. Hepatitis associated antigen. Green fluorescent protein. Lee, Khai Wooi Tan, Wen Siang Recombinant hepatitis B virus core particles: association, dissociation and encapsidation of green fluorescent protein. |
| title | Recombinant hepatitis B virus core particles: association, dissociation and encapsidation of green fluorescent protein. |
| title_full | Recombinant hepatitis B virus core particles: association, dissociation and encapsidation of green fluorescent protein. |
| title_fullStr | Recombinant hepatitis B virus core particles: association, dissociation and encapsidation of green fluorescent protein. |
| title_full_unstemmed | Recombinant hepatitis B virus core particles: association, dissociation and encapsidation of green fluorescent protein. |
| title_short | Recombinant hepatitis B virus core particles: association, dissociation and encapsidation of green fluorescent protein. |
| title_sort | recombinant hepatitis b virus core particles association dissociation and encapsidation of green fluorescent protein |
| topic | Hepatitis B virus. Hepatitis associated antigen. Green fluorescent protein. |
| url | http://psasir.upm.edu.my/id/eprint/16448/1/Recombinant%20hepatitis%20B%20virus%20core%20particles.pdf |
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