Screening for the optimal induction parameters for periplasmic producing interferon-α2b in Escherichia coli
Screening for optimum induction parameters to improve the production of periplasmic interferon-α2b (PrIFN-α2b) by recombinant Escherichia coli was conducted using shake flask culture. Recombinant E. coli Rosetta-gami 2(DE3) harboring the plasmid pET26b containing IFN-α2b gene under the control of th...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Academic Journals
2010
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/16630/1/16630.pdf |
| _version_ | 1796969092898357248 |
|---|---|
| author | Azaman, Siti Nor Ani Ramakrishnan, Nagasundara Ramanan Tan, Joo Shun Abdul Rahim, Raha Abdullah, Mohd Puad Ariff, Arbakariya |
| author_facet | Azaman, Siti Nor Ani Ramakrishnan, Nagasundara Ramanan Tan, Joo Shun Abdul Rahim, Raha Abdullah, Mohd Puad Ariff, Arbakariya |
| author_sort | Azaman, Siti Nor Ani |
| collection | UPM |
| description | Screening for optimum induction parameters to improve the production of periplasmic interferon-α2b (PrIFN-α2b) by recombinant Escherichia coli was conducted using shake flask culture. Recombinant E. coli Rosetta-gami 2(DE3) harboring the plasmid pET26b containing IFN-α2b gene under the control of the T7lac promoter was used, where the induction was accomplished by isopropyl β-D-1-thiogalactopyranoside (IPTG). The induction parameters (inducer concentration, point of induction, induction temperature and the length of induction) were analyzed to find the suitable range to be used for further optimization process. From the analysis, narrow range of induction temperature from 16 to 30°C and IPTG lower than 2 mM were found suitable for induction of PrIFN-α2b. On the other hand, early log phase was the preferred time to initiate the induction and the length of induction was dependent on the combination of other induction parameters used. |
| first_indexed | 2024-03-06T07:37:59Z |
| format | Article |
| id | upm.eprints-16630 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T07:37:59Z |
| publishDate | 2010 |
| publisher | Academic Journals |
| record_format | dspace |
| spelling | upm.eprints-166302017-11-09T04:39:31Z http://psasir.upm.edu.my/id/eprint/16630/ Screening for the optimal induction parameters for periplasmic producing interferon-α2b in Escherichia coli Azaman, Siti Nor Ani Ramakrishnan, Nagasundara Ramanan Tan, Joo Shun Abdul Rahim, Raha Abdullah, Mohd Puad Ariff, Arbakariya Screening for optimum induction parameters to improve the production of periplasmic interferon-α2b (PrIFN-α2b) by recombinant Escherichia coli was conducted using shake flask culture. Recombinant E. coli Rosetta-gami 2(DE3) harboring the plasmid pET26b containing IFN-α2b gene under the control of the T7lac promoter was used, where the induction was accomplished by isopropyl β-D-1-thiogalactopyranoside (IPTG). The induction parameters (inducer concentration, point of induction, induction temperature and the length of induction) were analyzed to find the suitable range to be used for further optimization process. From the analysis, narrow range of induction temperature from 16 to 30°C and IPTG lower than 2 mM were found suitable for induction of PrIFN-α2b. On the other hand, early log phase was the preferred time to initiate the induction and the length of induction was dependent on the combination of other induction parameters used. Academic Journals 2010 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/16630/1/16630.pdf Azaman, Siti Nor Ani and Ramakrishnan, Nagasundara Ramanan and Tan, Joo Shun and Abdul Rahim, Raha and Abdullah, Mohd Puad and Ariff, Arbakariya (2010) Screening for the optimal induction parameters for periplasmic producing interferon-α2b in Escherichia coli. African Journal of Biotechnology, 9 (38). art. no. 27DCBCA20675. pp. 6345-6354. ISSN 1684–5315 http://www.academicjournals.org/journal/AJB/article-abstract/27DCBCA20675 |
| spellingShingle | Azaman, Siti Nor Ani Ramakrishnan, Nagasundara Ramanan Tan, Joo Shun Abdul Rahim, Raha Abdullah, Mohd Puad Ariff, Arbakariya Screening for the optimal induction parameters for periplasmic producing interferon-α2b in Escherichia coli |
| title | Screening for the optimal induction parameters for periplasmic producing interferon-α2b in Escherichia coli |
| title_full | Screening for the optimal induction parameters for periplasmic producing interferon-α2b in Escherichia coli |
| title_fullStr | Screening for the optimal induction parameters for periplasmic producing interferon-α2b in Escherichia coli |
| title_full_unstemmed | Screening for the optimal induction parameters for periplasmic producing interferon-α2b in Escherichia coli |
| title_short | Screening for the optimal induction parameters for periplasmic producing interferon-α2b in Escherichia coli |
| title_sort | screening for the optimal induction parameters for periplasmic producing interferon α2b in escherichia coli |
| url | http://psasir.upm.edu.my/id/eprint/16630/1/16630.pdf |
| work_keys_str_mv | AT azamansitinorani screeningfortheoptimalinductionparametersforperiplasmicproducinginterferona2binescherichiacoli AT ramakrishnannagasundararamanan screeningfortheoptimalinductionparametersforperiplasmicproducinginterferona2binescherichiacoli AT tanjooshun screeningfortheoptimalinductionparametersforperiplasmicproducinginterferona2binescherichiacoli AT abdulrahimraha screeningfortheoptimalinductionparametersforperiplasmicproducinginterferona2binescherichiacoli AT abdullahmohdpuad screeningfortheoptimalinductionparametersforperiplasmicproducinginterferona2binescherichiacoli AT ariffarbakariya screeningfortheoptimalinductionparametersforperiplasmicproducinginterferona2binescherichiacoli |