A tapered fibre optics biosensor for histamine detection
Purpose: This paper aims to estimate the level of histamine in fish and fish products, as it is very important because of their implication in fish poisoning in humans; hence, ascertaining histamine levels in the aforementioned serves as a chemical index for spoilage. Design/methodology/approach: A...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Emerald Group Publishing
2016
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| Online Access: | http://psasir.upm.edu.my/id/eprint/16876/1/A%20tapered%20fibre%20optics%20biosensor%20for%20histamine%20detection.pdf |
| _version_ | 1825945900772491264 |
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| author | Usman, Hamza Abu Bakar, Muhammad Hafiz Amir Hamzah, Amir Syahir Salleh, Abu Bakar |
| author_facet | Usman, Hamza Abu Bakar, Muhammad Hafiz Amir Hamzah, Amir Syahir Salleh, Abu Bakar |
| author_sort | Usman, Hamza |
| collection | UPM |
| description | Purpose: This paper aims to estimate the level of histamine in fish and fish products, as it is very important because of their implication in fish poisoning in humans; hence, ascertaining histamine levels in the aforementioned serves as a chemical index for spoilage. Design/methodology/approach: A technique was developed to immobilize an ordered multilayer of diamine oxidase (DAO) by means of chemical cross-linking on the biconical taper surface stepwisely alternating between chitosan, glutaraldehyde and the enzyme. A spectrophotometric signal results from horseradish peroxidase catalyzed reduction of H2O2, a secondary product of the oxidative deamination of histamine monitored at 450 nm. Findings: The biosensor showed a linear response range up to 1.5 mM, a good sensitivity of 0.64 mM-1 with detection and quantification limits towards histamine of 0.086 mM (15.8 ppm) and 0.204 mM (37.7 ppm) and a linear response range of 0-1.5 mM. It showed a response and recovery time of 14 sec and operational stability up to 40 repeated analyses without significant loss of sensitivity. Practical implications: The developed biosensor has a good potential for use in the quantitative determination of histamine in seafood. Originality/value: The paper described an outcome of an experimental work on tapered fibre optics (taper)-based biosensor coated with DAO embedded into a chitosan membrane to measure histamine. |
| first_indexed | 2024-03-06T07:38:43Z |
| format | Article |
| id | upm.eprints-16876 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T07:38:43Z |
| publishDate | 2016 |
| publisher | Emerald Group Publishing |
| record_format | dspace |
| spelling | upm.eprints-168762016-06-08T02:09:37Z http://psasir.upm.edu.my/id/eprint/16876/ A tapered fibre optics biosensor for histamine detection Usman, Hamza Abu Bakar, Muhammad Hafiz Amir Hamzah, Amir Syahir Salleh, Abu Bakar Purpose: This paper aims to estimate the level of histamine in fish and fish products, as it is very important because of their implication in fish poisoning in humans; hence, ascertaining histamine levels in the aforementioned serves as a chemical index for spoilage. Design/methodology/approach: A technique was developed to immobilize an ordered multilayer of diamine oxidase (DAO) by means of chemical cross-linking on the biconical taper surface stepwisely alternating between chitosan, glutaraldehyde and the enzyme. A spectrophotometric signal results from horseradish peroxidase catalyzed reduction of H2O2, a secondary product of the oxidative deamination of histamine monitored at 450 nm. Findings: The biosensor showed a linear response range up to 1.5 mM, a good sensitivity of 0.64 mM-1 with detection and quantification limits towards histamine of 0.086 mM (15.8 ppm) and 0.204 mM (37.7 ppm) and a linear response range of 0-1.5 mM. It showed a response and recovery time of 14 sec and operational stability up to 40 repeated analyses without significant loss of sensitivity. Practical implications: The developed biosensor has a good potential for use in the quantitative determination of histamine in seafood. Originality/value: The paper described an outcome of an experimental work on tapered fibre optics (taper)-based biosensor coated with DAO embedded into a chitosan membrane to measure histamine. Emerald Group Publishing 2016 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/16876/1/A%20tapered%20fibre%20optics%20biosensor%20for%20histamine%20detection.pdf Usman, Hamza and Abu Bakar, Muhammad Hafiz and Amir Hamzah, Amir Syahir and Salleh, Abu Bakar (2016) A tapered fibre optics biosensor for histamine detection. Sensor Review, 36 (1). pp. 40-47. ISSN 0260-2288 http://www.emeraldinsight.com/doi/abs/10.1108/SR-06-2015-0101 10.1108/SR-06-2015-0101 |
| spellingShingle | Usman, Hamza Abu Bakar, Muhammad Hafiz Amir Hamzah, Amir Syahir Salleh, Abu Bakar A tapered fibre optics biosensor for histamine detection |
| title | A tapered fibre optics biosensor for histamine detection |
| title_full | A tapered fibre optics biosensor for histamine detection |
| title_fullStr | A tapered fibre optics biosensor for histamine detection |
| title_full_unstemmed | A tapered fibre optics biosensor for histamine detection |
| title_short | A tapered fibre optics biosensor for histamine detection |
| title_sort | tapered fibre optics biosensor for histamine detection |
| url | http://psasir.upm.edu.my/id/eprint/16876/1/A%20tapered%20fibre%20optics%20biosensor%20for%20histamine%20detection.pdf |
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