Synbiotics growth optimization of Bifidobacterium pseudocatenulatum G4 with prebiotics using a statistical methodology
Aims: This study demonstrated the optimum growth of Bifidobacterium pseudocatenulatum G4 with prebiotics via statistical model.Methods and Results: Commercial prebiotics [inulin and fructooligosaccharide (FOS)], together with sorbitol, arabinan and inoculum rate, were tested by fractional factoria...
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| Format: | Article |
| Language: | English English |
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Wiley Blackwell
2009
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| Online Access: | http://psasir.upm.edu.my/id/eprint/16917/1/Synbiotics%20growth%20optimization%20of%20Bifidobacterium%20pseudocatenulatum%20G4%20with%20prebiotics%20using%20a%20statistical%20methodology.pdf |
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| author | Mustafa, Shuhaimi B.M., Kabeir Abd. Manap, Mohd. Yazid Abdullah, Muhammad Nazrul Hakim |
| author_facet | Mustafa, Shuhaimi B.M., Kabeir Abd. Manap, Mohd. Yazid Abdullah, Muhammad Nazrul Hakim |
| author_sort | Mustafa, Shuhaimi |
| collection | UPM |
| description | Aims: This study demonstrated the optimum growth of Bifidobacterium pseudocatenulatum G4 with prebiotics via statistical model.Methods and Results: Commercial prebiotics [inulin and fructooligosaccharide (FOS)], together with sorbitol, arabinan and inoculum rate, were tested by fractional factorial design to determine their impact on growth of Bif. pseudocatenulatum G4 in skim milk. At 48 h incubation, bacterial growth was mainly influenced by FOS and inoculum rate. Growth reduction was observed in all samples incubated for 72 h. Central composite design (CCD) was adopted using FOS and inoculum rate at 48 h incubation to develop the statistical model for optimization. The model predicted that 2·461 log CFU ml−1 produced the optimum growth increase of Bif. pseudocatenulatum G4. The combination that produced the optimum point was 2·86% FOS (g/v) and 0·67% inoculum rate (v/v).Conclusion: At optimum combination of inoculum rate and FOS, validation experiments recorded 2·40 ± 10·02 log CFU ml−1. The application in 1-l bioreactor for 24 h showed higher growth increase of 2·95 log CFU ml−1.Significant and Impact of the Study: Response surface methodology approach is useful to develop optimum synbiotics combination for strain G4 with FOS. |
| first_indexed | 2024-03-06T07:38:50Z |
| format | Article |
| id | upm.eprints-16917 |
| institution | Universiti Putra Malaysia |
| language | English English |
| last_indexed | 2024-03-06T07:38:50Z |
| publishDate | 2009 |
| publisher | Wiley Blackwell |
| record_format | dspace |
| spelling | upm.eprints-169172015-10-23T01:54:36Z http://psasir.upm.edu.my/id/eprint/16917/ Synbiotics growth optimization of Bifidobacterium pseudocatenulatum G4 with prebiotics using a statistical methodology Mustafa, Shuhaimi B.M., Kabeir Abd. Manap, Mohd. Yazid Abdullah, Muhammad Nazrul Hakim Aims: This study demonstrated the optimum growth of Bifidobacterium pseudocatenulatum G4 with prebiotics via statistical model.Methods and Results: Commercial prebiotics [inulin and fructooligosaccharide (FOS)], together with sorbitol, arabinan and inoculum rate, were tested by fractional factorial design to determine their impact on growth of Bif. pseudocatenulatum G4 in skim milk. At 48 h incubation, bacterial growth was mainly influenced by FOS and inoculum rate. Growth reduction was observed in all samples incubated for 72 h. Central composite design (CCD) was adopted using FOS and inoculum rate at 48 h incubation to develop the statistical model for optimization. The model predicted that 2·461 log CFU ml−1 produced the optimum growth increase of Bif. pseudocatenulatum G4. The combination that produced the optimum point was 2·86% FOS (g/v) and 0·67% inoculum rate (v/v).Conclusion: At optimum combination of inoculum rate and FOS, validation experiments recorded 2·40 ± 10·02 log CFU ml−1. The application in 1-l bioreactor for 24 h showed higher growth increase of 2·95 log CFU ml−1.Significant and Impact of the Study: Response surface methodology approach is useful to develop optimum synbiotics combination for strain G4 with FOS. Wiley Blackwell 2009 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/16917/1/Synbiotics%20growth%20optimization%20of%20Bifidobacterium%20pseudocatenulatum%20G4%20with%20prebiotics%20using%20a%20statistical%20methodology.pdf Mustafa, Shuhaimi and B.M., Kabeir and Abd. Manap, Mohd. Yazid and Abdullah, Muhammad Nazrul Hakim (2009) Synbiotics growth optimization of Bifidobacterium pseudocatenulatum G4 with prebiotics using a statistical methodology. Journal of Applied Microbiology, 106 (1). pp. 191-198. ISSN 1364-5072 Bifidobacterium 10.1111/j.1365-2672.2008.03991.x English |
| spellingShingle | Bifidobacterium Mustafa, Shuhaimi B.M., Kabeir Abd. Manap, Mohd. Yazid Abdullah, Muhammad Nazrul Hakim Synbiotics growth optimization of Bifidobacterium pseudocatenulatum G4 with prebiotics using a statistical methodology |
| title | Synbiotics growth optimization of Bifidobacterium pseudocatenulatum G4 with prebiotics using a statistical methodology |
| title_full | Synbiotics growth optimization of Bifidobacterium pseudocatenulatum G4 with prebiotics using a statistical methodology |
| title_fullStr | Synbiotics growth optimization of Bifidobacterium pseudocatenulatum G4 with prebiotics using a statistical methodology |
| title_full_unstemmed | Synbiotics growth optimization of Bifidobacterium pseudocatenulatum G4 with prebiotics using a statistical methodology |
| title_short | Synbiotics growth optimization of Bifidobacterium pseudocatenulatum G4 with prebiotics using a statistical methodology |
| title_sort | synbiotics growth optimization of bifidobacterium pseudocatenulatum g4 with prebiotics using a statistical methodology |
| topic | Bifidobacterium |
| url | http://psasir.upm.edu.my/id/eprint/16917/1/Synbiotics%20growth%20optimization%20of%20Bifidobacterium%20pseudocatenulatum%20G4%20with%20prebiotics%20using%20a%20statistical%20methodology.pdf |
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