Synthesis, characterization, and structural properties of intracellular copolyester poly(3-hydroxybutyrate-co-3-hydroxyvalerate) produced by Comamonas sp. EB 172 from renewable resource
Microbial copolymer was produced by a local isolate, Comamonas sp. EB 172, using mixed organic acids such as acetic, propionic, and butyric acids as carbon sources in pH-stat fed-batch fermentation. Maximum polymer production (6.59 g/L) was achieved at 50 h of fermentation when 73.64 g/L mixed acids...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis
2010
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/16956/1/Synthesis%2C%20characterization%20and%20structural%20properties%20of%20intracellular%20copolyester%20poly%283-hydroxybutyrate-co-%203-%20hydroxyvalerate%29%20produced%20by%20Comamonas%20sp.%20EB%20172%20from%20renewable%20resource..pdf |
| _version_ | 1825945916277784576 |
|---|---|
| author | Mumtaz, Tabassum Abd. Aziz, Suraini Phang, Lai Yee Wan Yunus, Wan Md. Zin Shirai, Yoshihito Hassan, Mohd Ali |
| author_facet | Mumtaz, Tabassum Abd. Aziz, Suraini Phang, Lai Yee Wan Yunus, Wan Md. Zin Shirai, Yoshihito Hassan, Mohd Ali |
| author_sort | Mumtaz, Tabassum |
| collection | UPM |
| description | Microbial copolymer was produced by a local isolate, Comamonas sp. EB 172, using mixed organic acids such as acetic, propionic, and butyric acids as carbon sources in pH-stat fed-batch fermentation. Maximum polymer production (6.59 g/L) was achieved at 50 h of fermentation when 73.64 g/L mixed acids, generated from the acidogenic fermentation of palm oil mill wastewater, were used. Accumulation of polymer in the cell was 70% (wt/wt), which was observed under transmission electron microscope. The morphological, chemical, thermal, and mechanical properties of the solvent-extracted biopolymer were determined by various techniques (SEM, GC, 13C NMR, FT-IR, TGA, and tensile testing). The copolymer poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) consisted of 87 mol% β-hydroxybutyric acid (HB) and 13 mol% β-hydroxyvaleric acid (HV). With chemical properties similar to commercial PHBV and mechanical strength of around 30 MPa and 8% elongation at break, the biopolymer offers potential for industrial applications. |
| first_indexed | 2024-03-06T07:38:57Z |
| format | Article |
| id | upm.eprints-16956 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T07:38:57Z |
| publishDate | 2010 |
| publisher | Taylor & Francis |
| record_format | dspace |
| spelling | upm.eprints-169562017-05-16T09:56:55Z http://psasir.upm.edu.my/id/eprint/16956/ Synthesis, characterization, and structural properties of intracellular copolyester poly(3-hydroxybutyrate-co-3-hydroxyvalerate) produced by Comamonas sp. EB 172 from renewable resource Mumtaz, Tabassum Abd. Aziz, Suraini Phang, Lai Yee Wan Yunus, Wan Md. Zin Shirai, Yoshihito Hassan, Mohd Ali Microbial copolymer was produced by a local isolate, Comamonas sp. EB 172, using mixed organic acids such as acetic, propionic, and butyric acids as carbon sources in pH-stat fed-batch fermentation. Maximum polymer production (6.59 g/L) was achieved at 50 h of fermentation when 73.64 g/L mixed acids, generated from the acidogenic fermentation of palm oil mill wastewater, were used. Accumulation of polymer in the cell was 70% (wt/wt), which was observed under transmission electron microscope. The morphological, chemical, thermal, and mechanical properties of the solvent-extracted biopolymer were determined by various techniques (SEM, GC, 13C NMR, FT-IR, TGA, and tensile testing). The copolymer poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) consisted of 87 mol% β-hydroxybutyric acid (HB) and 13 mol% β-hydroxyvaleric acid (HV). With chemical properties similar to commercial PHBV and mechanical strength of around 30 MPa and 8% elongation at break, the biopolymer offers potential for industrial applications. Taylor & Francis 2010 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/16956/1/Synthesis%2C%20characterization%20and%20structural%20properties%20of%20intracellular%20copolyester%20poly%283-hydroxybutyrate-co-%203-%20hydroxyvalerate%29%20produced%20by%20Comamonas%20sp.%20EB%20172%20from%20renewable%20resource..pdf Mumtaz, Tabassum and Abd. Aziz, Suraini and Phang, Lai Yee and Wan Yunus, Wan Md. Zin and Shirai, Yoshihito and Hassan, Mohd Ali (2010) Synthesis, characterization, and structural properties of intracellular copolyester poly(3-hydroxybutyrate-co-3-hydroxyvalerate) produced by Comamonas sp. EB 172 from renewable resource. International Journal of Polymer Analysis and Characterization, 15 (6). pp. 329-340. ISSN 1023-666X http://www.tandfonline.com/doi/abs/10.1080/1023666X.2010.500522 10.1080/1023666X.2010.500522 |
| spellingShingle | Mumtaz, Tabassum Abd. Aziz, Suraini Phang, Lai Yee Wan Yunus, Wan Md. Zin Shirai, Yoshihito Hassan, Mohd Ali Synthesis, characterization, and structural properties of intracellular copolyester poly(3-hydroxybutyrate-co-3-hydroxyvalerate) produced by Comamonas sp. EB 172 from renewable resource |
| title | Synthesis, characterization, and structural properties of intracellular copolyester poly(3-hydroxybutyrate-co-3-hydroxyvalerate) produced by Comamonas sp. EB 172 from renewable resource |
| title_full | Synthesis, characterization, and structural properties of intracellular copolyester poly(3-hydroxybutyrate-co-3-hydroxyvalerate) produced by Comamonas sp. EB 172 from renewable resource |
| title_fullStr | Synthesis, characterization, and structural properties of intracellular copolyester poly(3-hydroxybutyrate-co-3-hydroxyvalerate) produced by Comamonas sp. EB 172 from renewable resource |
| title_full_unstemmed | Synthesis, characterization, and structural properties of intracellular copolyester poly(3-hydroxybutyrate-co-3-hydroxyvalerate) produced by Comamonas sp. EB 172 from renewable resource |
| title_short | Synthesis, characterization, and structural properties of intracellular copolyester poly(3-hydroxybutyrate-co-3-hydroxyvalerate) produced by Comamonas sp. EB 172 from renewable resource |
| title_sort | synthesis characterization and structural properties of intracellular copolyester poly 3 hydroxybutyrate co 3 hydroxyvalerate produced by comamonas sp eb 172 from renewable resource |
| url | http://psasir.upm.edu.my/id/eprint/16956/1/Synthesis%2C%20characterization%20and%20structural%20properties%20of%20intracellular%20copolyester%20poly%283-hydroxybutyrate-co-%203-%20hydroxyvalerate%29%20produced%20by%20Comamonas%20sp.%20EB%20172%20from%20renewable%20resource..pdf |
| work_keys_str_mv | AT mumtaztabassum synthesischaracterizationandstructuralpropertiesofintracellularcopolyesterpoly3hydroxybutyrateco3hydroxyvalerateproducedbycomamonasspeb172fromrenewableresource AT abdazizsuraini synthesischaracterizationandstructuralpropertiesofintracellularcopolyesterpoly3hydroxybutyrateco3hydroxyvalerateproducedbycomamonasspeb172fromrenewableresource AT phanglaiyee synthesischaracterizationandstructuralpropertiesofintracellularcopolyesterpoly3hydroxybutyrateco3hydroxyvalerateproducedbycomamonasspeb172fromrenewableresource AT wanyunuswanmdzin synthesischaracterizationandstructuralpropertiesofintracellularcopolyesterpoly3hydroxybutyrateco3hydroxyvalerateproducedbycomamonasspeb172fromrenewableresource AT shiraiyoshihito synthesischaracterizationandstructuralpropertiesofintracellularcopolyesterpoly3hydroxybutyrateco3hydroxyvalerateproducedbycomamonasspeb172fromrenewableresource AT hassanmohdali synthesischaracterizationandstructuralpropertiesofintracellularcopolyesterpoly3hydroxybutyrateco3hydroxyvalerateproducedbycomamonasspeb172fromrenewableresource |