Oral vaccine of Lactococcus lactis harbouring pandemic H1N1 2009 haemagglutinin1 and nisP anchor fusion protein elevates anti-HA1 sIgA levels in mice
Objective: An oral lactococcal-based vaccine which haboured the haemagglutinin1 (HA1) antigen fused to nisP anchor protein for the purpose of surface displaying the HA1 antigen was developed against H1N1 virus. Results: Recombinant L. lactis strains expressed HA1-nisP fusion proteins when induced wi...
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| Format: | Article |
| Language: | English |
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Springer
2016
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| Online Access: | http://psasir.upm.edu.my/id/eprint/16997/1/Oral%20vaccine%20of%20Lactococcus%20lactis%20harbouring%20pandemic%20H1N1%202009%20haemagglutinin1%20and%20nisP%20anchor%20fusion%20protein%20elevates%20anti-HA1%20sIgA%20levels%20in%20mice.pdf |
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| author | Siaw, Stella Xiu Joan Jee, Pui Fong Song, Adelene Ai Lian Chang, Li Yen Yusoff, Khatijah Abu Bakar, Sazaly Abdul Rahim, Raha |
| author_facet | Siaw, Stella Xiu Joan Jee, Pui Fong Song, Adelene Ai Lian Chang, Li Yen Yusoff, Khatijah Abu Bakar, Sazaly Abdul Rahim, Raha |
| author_sort | Siaw, Stella Xiu Joan |
| collection | UPM |
| description | Objective: An oral lactococcal-based vaccine which haboured the haemagglutinin1 (HA1) antigen fused to nisP anchor protein for the purpose of surface displaying the HA1 antigen was developed against H1N1 virus. Results: Recombinant L. lactis strains expressed HA1-nisP fusion proteins when induced with nisin, as confirmed through western blotting. However, immunofluorescense did not detect any surface-displayed proteins, suggesting that the protein was either unsuccessfully translocated or improperly displayed. Despite this, oral administration of recombinant L. lactis strains to BALB/c mice revealed that significant levels of anti-HA1 sIgA antibodies were detected in mice fecal suspension samples of mice group NZ9000 (pNZ:HN) when compared to the negative control NZ9000 (pNZ8048) group. Conclusion: Specific anti-HA1 sIgA antibodies were locally produced and live recombinant lactococcal vaccine was able to elicit humoral response of BALB/c mice despite unsuccessful surface display of the HA1 epitope. |
| first_indexed | 2024-03-06T07:39:05Z |
| format | Article |
| id | upm.eprints-16997 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T07:39:05Z |
| publishDate | 2016 |
| publisher | Springer |
| record_format | dspace |
| spelling | upm.eprints-169972016-06-08T04:06:50Z http://psasir.upm.edu.my/id/eprint/16997/ Oral vaccine of Lactococcus lactis harbouring pandemic H1N1 2009 haemagglutinin1 and nisP anchor fusion protein elevates anti-HA1 sIgA levels in mice Siaw, Stella Xiu Joan Jee, Pui Fong Song, Adelene Ai Lian Chang, Li Yen Yusoff, Khatijah Abu Bakar, Sazaly Abdul Rahim, Raha Objective: An oral lactococcal-based vaccine which haboured the haemagglutinin1 (HA1) antigen fused to nisP anchor protein for the purpose of surface displaying the HA1 antigen was developed against H1N1 virus. Results: Recombinant L. lactis strains expressed HA1-nisP fusion proteins when induced with nisin, as confirmed through western blotting. However, immunofluorescense did not detect any surface-displayed proteins, suggesting that the protein was either unsuccessfully translocated or improperly displayed. Despite this, oral administration of recombinant L. lactis strains to BALB/c mice revealed that significant levels of anti-HA1 sIgA antibodies were detected in mice fecal suspension samples of mice group NZ9000 (pNZ:HN) when compared to the negative control NZ9000 (pNZ8048) group. Conclusion: Specific anti-HA1 sIgA antibodies were locally produced and live recombinant lactococcal vaccine was able to elicit humoral response of BALB/c mice despite unsuccessful surface display of the HA1 epitope. Springer 2016 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/16997/1/Oral%20vaccine%20of%20Lactococcus%20lactis%20harbouring%20pandemic%20H1N1%202009%20haemagglutinin1%20and%20nisP%20anchor%20fusion%20protein%20elevates%20anti-HA1%20sIgA%20levels%20in%20mice.pdf Siaw, Stella Xiu Joan and Jee, Pui Fong and Song, Adelene Ai Lian and Chang, Li Yen and Yusoff, Khatijah and Abu Bakar, Sazaly and Abdul Rahim, Raha (2016) Oral vaccine of Lactococcus lactis harbouring pandemic H1N1 2009 haemagglutinin1 and nisP anchor fusion protein elevates anti-HA1 sIgA levels in mice. Biotechnology Letters, 38 (5). pp. 793-799. ISSN 0141-5492; ESSN: 1573-6776 http://link.springer.com/article/10.1007%2Fs10529-016-2034-2 10.1007/s10529-016-2034-2 |
| spellingShingle | Siaw, Stella Xiu Joan Jee, Pui Fong Song, Adelene Ai Lian Chang, Li Yen Yusoff, Khatijah Abu Bakar, Sazaly Abdul Rahim, Raha Oral vaccine of Lactococcus lactis harbouring pandemic H1N1 2009 haemagglutinin1 and nisP anchor fusion protein elevates anti-HA1 sIgA levels in mice |
| title | Oral vaccine of Lactococcus lactis harbouring pandemic H1N1 2009 haemagglutinin1 and nisP anchor fusion protein elevates anti-HA1 sIgA levels in mice |
| title_full | Oral vaccine of Lactococcus lactis harbouring pandemic H1N1 2009 haemagglutinin1 and nisP anchor fusion protein elevates anti-HA1 sIgA levels in mice |
| title_fullStr | Oral vaccine of Lactococcus lactis harbouring pandemic H1N1 2009 haemagglutinin1 and nisP anchor fusion protein elevates anti-HA1 sIgA levels in mice |
| title_full_unstemmed | Oral vaccine of Lactococcus lactis harbouring pandemic H1N1 2009 haemagglutinin1 and nisP anchor fusion protein elevates anti-HA1 sIgA levels in mice |
| title_short | Oral vaccine of Lactococcus lactis harbouring pandemic H1N1 2009 haemagglutinin1 and nisP anchor fusion protein elevates anti-HA1 sIgA levels in mice |
| title_sort | oral vaccine of lactococcus lactis harbouring pandemic h1n1 2009 haemagglutinin1 and nisp anchor fusion protein elevates anti ha1 siga levels in mice |
| url | http://psasir.upm.edu.my/id/eprint/16997/1/Oral%20vaccine%20of%20Lactococcus%20lactis%20harbouring%20pandemic%20H1N1%202009%20haemagglutinin1%20and%20nisP%20anchor%20fusion%20protein%20elevates%20anti-HA1%20sIgA%20levels%20in%20mice.pdf |
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