The effects of microgravity on thermostable T1 lipase protein crystal
The quest for the characterizations of intrinsically thermostable T1 lipase either physicochemically or structurally is a prominent task. T1 lipase can be effectively used as an additive in detergent formulations, and as a biocatalyst for natural oil-based pharmaceuticals, foods and fine chemicals....
| Main Authors: | , , , , , |
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| Formato: | Artigo |
| Idioma: | English |
| Publicado: |
American Society for Gravitational and Space Biology
2010
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| Acceso en liña: | http://psasir.upm.edu.my/id/eprint/17137/1/17137.pdf |
| _version_ | 1825945951774179328 |
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| author | Raja Abdul Rahman, Raja Noor Zaliha Mohamad Ali, Mohd Shukuri Leow, Thean Chor Salleh, Abu Bakar Basri, Mahiran Matsumura, H. |
| author_facet | Raja Abdul Rahman, Raja Noor Zaliha Mohamad Ali, Mohd Shukuri Leow, Thean Chor Salleh, Abu Bakar Basri, Mahiran Matsumura, H. |
| author_sort | Raja Abdul Rahman, Raja Noor Zaliha |
| collection | UPM |
| description | The quest for the characterizations of intrinsically thermostable T1 lipase either physicochemically or structurally is a prominent task. T1 lipase can be effectively used as an additive in detergent formulations, and as a biocatalyst for natural oil-based pharmaceuticals, foods and fine chemicals. The thermoalkaliphilic T1 lipase gene of Geobacillus zalihae sp. nov. strain T1 T (Rahman et al., 2007) was overexpressed in the pGEX vector in E. coli (Loew et al., 2004). Expression of T1 lipase as a glutathione S-transferase (GST) fusion protein in prokaryotic systems was expected to allow rapid purification of recombinant T1 lipase through affinity chromatography. High-yield purification of T1 lipase was achieved through two-step affinity chromatography with a final specific activity and yield of 958.2 U/mg and 51.5%, respectively. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis showed that the purified T1 lipase appeared as 39 kDa after the removal of the 26 kDa GST tag from the digested fusion lipase. However, the native molecular weight of T1 lipase was determined to be approximately 43 kDa by gel filtration chromatography. The size was similar to its predicted molecular weight, but slightly bigger than its denatured form obtained through SDS-PAGE (Leow et al., 2007). |
| first_indexed | 2024-03-06T07:39:32Z |
| format | Article |
| id | upm.eprints-17137 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T07:39:32Z |
| publishDate | 2010 |
| publisher | American Society for Gravitational and Space Biology |
| record_format | dspace |
| spelling | upm.eprints-171372016-09-26T05:18:06Z http://psasir.upm.edu.my/id/eprint/17137/ The effects of microgravity on thermostable T1 lipase protein crystal Raja Abdul Rahman, Raja Noor Zaliha Mohamad Ali, Mohd Shukuri Leow, Thean Chor Salleh, Abu Bakar Basri, Mahiran Matsumura, H. The quest for the characterizations of intrinsically thermostable T1 lipase either physicochemically or structurally is a prominent task. T1 lipase can be effectively used as an additive in detergent formulations, and as a biocatalyst for natural oil-based pharmaceuticals, foods and fine chemicals. The thermoalkaliphilic T1 lipase gene of Geobacillus zalihae sp. nov. strain T1 T (Rahman et al., 2007) was overexpressed in the pGEX vector in E. coli (Loew et al., 2004). Expression of T1 lipase as a glutathione S-transferase (GST) fusion protein in prokaryotic systems was expected to allow rapid purification of recombinant T1 lipase through affinity chromatography. High-yield purification of T1 lipase was achieved through two-step affinity chromatography with a final specific activity and yield of 958.2 U/mg and 51.5%, respectively. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis showed that the purified T1 lipase appeared as 39 kDa after the removal of the 26 kDa GST tag from the digested fusion lipase. However, the native molecular weight of T1 lipase was determined to be approximately 43 kDa by gel filtration chromatography. The size was similar to its predicted molecular weight, but slightly bigger than its denatured form obtained through SDS-PAGE (Leow et al., 2007). American Society for Gravitational and Space Biology 2010 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/17137/1/17137.pdf Raja Abdul Rahman, Raja Noor Zaliha and Mohamad Ali, Mohd Shukuri and Leow, Thean Chor and Salleh, Abu Bakar and Basri, Mahiran and Matsumura, H. (2010) The effects of microgravity on thermostable T1 lipase protein crystal. Gravitational and Space Biology, 23 (2). pp. 89-90. ISSN 2332-7774 http://gravitationalandspacebiology.org/index.php/journal/article/view/500 |
| spellingShingle | Raja Abdul Rahman, Raja Noor Zaliha Mohamad Ali, Mohd Shukuri Leow, Thean Chor Salleh, Abu Bakar Basri, Mahiran Matsumura, H. The effects of microgravity on thermostable T1 lipase protein crystal |
| title | The effects of microgravity on thermostable T1 lipase protein crystal |
| title_full | The effects of microgravity on thermostable T1 lipase protein crystal |
| title_fullStr | The effects of microgravity on thermostable T1 lipase protein crystal |
| title_full_unstemmed | The effects of microgravity on thermostable T1 lipase protein crystal |
| title_short | The effects of microgravity on thermostable T1 lipase protein crystal |
| title_sort | effects of microgravity on thermostable t1 lipase protein crystal |
| url | http://psasir.upm.edu.my/id/eprint/17137/1/17137.pdf |
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