The performance of a glass bead shaking technique for the disruption of Escherichia coli cells
The efficacy of a simple laboratory method for cell disruption based on the shaking of glass beads on a rotary shaker was assessed in this study, via measurements of the release of total protein and interferon-α2b from E.coli. The optimum conditions for cell disruption were detected after 30 min of...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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The Korean Society for Biotechnology and Bioengineering
2008
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| Online Access: | http://psasir.upm.edu.my/id/eprint/17258/1/The%20performance%20of%20a%20glass%20bead%20shaking%20technique%20for%20the%20disruption%20of%20Escherichia%20coli%20cells.pdf |
| _version_ | 1796969219869376512 |
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| author | Ramakrishnan, Nagasundara Ramanan Tau, Chuan Ling Ariff, Arbakariya |
| author_facet | Ramakrishnan, Nagasundara Ramanan Tau, Chuan Ling Ariff, Arbakariya |
| author_sort | Ramakrishnan, Nagasundara Ramanan |
| collection | UPM |
| description | The efficacy of a simple laboratory method for cell disruption based on the shaking of glass beads on a rotary shaker was assessed in this study, via measurements of the release of total protein and interferon-α2b from E.coli. The optimum conditions for cell disruption were detected after 30 min of shaking in Tris-HCl buffer (pH 8) at 300 rpm with 1.5g of glass beads (diameter: 0.5 mm) per mL of cell suspension volume. Three test runs were conducted under the above conditions and the maximum average protein release values were determined as 3.048, 3.564, and 3.015
mg/mL, respectively. The amount of protein release was comparable to the amount of protein release in ultrasonication and glass bead vortexing procedures. The amount of interferon-α2b release in the ultrasonication, glass bead vortexing, and glass bead shaking trials were 240, 172, and 201 ng/mL, respectively. This method was shown to process between 1 and 10 mL of sample volume in a 50 mL Falcon tube without a great deal of deviation, and was
able to handle in excess of 60 samples simultaneously. |
| first_indexed | 2024-03-06T07:39:53Z |
| format | Article |
| id | upm.eprints-17258 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T07:39:53Z |
| publishDate | 2008 |
| publisher | The Korean Society for Biotechnology and Bioengineering |
| record_format | dspace |
| spelling | upm.eprints-172582016-07-22T03:21:47Z http://psasir.upm.edu.my/id/eprint/17258/ The performance of a glass bead shaking technique for the disruption of Escherichia coli cells Ramakrishnan, Nagasundara Ramanan Tau, Chuan Ling Ariff, Arbakariya The efficacy of a simple laboratory method for cell disruption based on the shaking of glass beads on a rotary shaker was assessed in this study, via measurements of the release of total protein and interferon-α2b from E.coli. The optimum conditions for cell disruption were detected after 30 min of shaking in Tris-HCl buffer (pH 8) at 300 rpm with 1.5g of glass beads (diameter: 0.5 mm) per mL of cell suspension volume. Three test runs were conducted under the above conditions and the maximum average protein release values were determined as 3.048, 3.564, and 3.015 mg/mL, respectively. The amount of protein release was comparable to the amount of protein release in ultrasonication and glass bead vortexing procedures. The amount of interferon-α2b release in the ultrasonication, glass bead vortexing, and glass bead shaking trials were 240, 172, and 201 ng/mL, respectively. This method was shown to process between 1 and 10 mL of sample volume in a 50 mL Falcon tube without a great deal of deviation, and was able to handle in excess of 60 samples simultaneously. The Korean Society for Biotechnology and Bioengineering 2008 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/17258/1/The%20performance%20of%20a%20glass%20bead%20shaking%20technique%20for%20the%20disruption%20of%20Escherichia%20coli%20cells.pdf Ramakrishnan, Nagasundara Ramanan and Tau, Chuan Ling and Ariff, Arbakariya (2008) The performance of a glass bead shaking technique for the disruption of Escherichia coli cells. Biotechnology and Bioprocess Engineering, 13 (5). pp. 613-623. ISSN 1226-8372 Escherichia coli Proteins - isolation & purification DOI/10.1007/s12257-008-0047-y |
| spellingShingle | Escherichia coli Proteins - isolation & purification Ramakrishnan, Nagasundara Ramanan Tau, Chuan Ling Ariff, Arbakariya The performance of a glass bead shaking technique for the disruption of Escherichia coli cells |
| title | The performance of a glass bead shaking technique for the disruption of Escherichia coli cells |
| title_full | The performance of a glass bead shaking technique for the disruption of Escherichia coli cells |
| title_fullStr | The performance of a glass bead shaking technique for the disruption of Escherichia coli cells |
| title_full_unstemmed | The performance of a glass bead shaking technique for the disruption of Escherichia coli cells |
| title_short | The performance of a glass bead shaking technique for the disruption of Escherichia coli cells |
| title_sort | performance of a glass bead shaking technique for the disruption of escherichia coli cells |
| topic | Escherichia coli Proteins - isolation & purification |
| url | http://psasir.upm.edu.my/id/eprint/17258/1/The%20performance%20of%20a%20glass%20bead%20shaking%20technique%20for%20the%20disruption%20of%20Escherichia%20coli%20cells.pdf |
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