The resin-embedded cornea prepared via rapid processing protocol : a good histomorphometric target for clinical investigation in opthalmology and optometry.
This study illustrates and quantifies the changes on corneal tissue between the paraffin-embedded and resin-embedded blocks and thus, selects a better target in investigational ophthalmology and optometry via light microscopy. Corneas of two cynomolgus monkeys (Macaca fascicularis) were used in this...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English English |
| Published: |
Penerbit Usm
2008
|
| Subjects: | |
| Online Access: | http://psasir.upm.edu.my/id/eprint/17310/1/The%20resin.pdf |
| _version_ | 1796969230421196800 |
|---|---|
| author | Cheah , Pike See Mohidin, Norhani Mohd Ali, Bariah Maung , Myint Abdul Latif , Azian |
| author_facet | Cheah , Pike See Mohidin, Norhani Mohd Ali, Bariah Maung , Myint Abdul Latif , Azian |
| author_sort | Cheah , Pike See |
| collection | UPM |
| description | This study illustrates and quantifies the changes on corneal tissue between the paraffin-embedded and resin-embedded blocks and thus, selects a better target in investigational ophthalmology and optometry via light microscopy. Corneas of two cynomolgus monkeys (Macaca fascicularis) were used in this study. The formalin-fixed cornea was prepared in paraffin block via the conventional tissue processing protocol (4-day protocol) and stained with haematoxylin and eosin. The glutaraldehyde-fixed cornea was prepared in resin block via the rapid and modified tissue processing procedure (1.2-day protocol) and stained with toluidine blue. The paraffin-embedded sample exhibits various undesired tissue damage and artifact such as thinner epithelium (due to the substantial volumic extraction from the tissue), thicker stroma layer (due to the separation of lamellae and the presence of voids) and the distorted endothelium. In contrast, the resin-embedded corneal tissue has demonstrated satisfactory corneal ultrastructural preservation. The rapid and modified tissue processing method for preparing the resin-embedded is particularly beneficial to accelerate the microscopic evaluation in ophthalmology and optometry. |
| first_indexed | 2024-03-06T07:40:03Z |
| format | Article |
| id | upm.eprints-17310 |
| institution | Universiti Putra Malaysia |
| language | English English |
| last_indexed | 2024-03-06T07:40:03Z |
| publishDate | 2008 |
| publisher | Penerbit Usm |
| record_format | dspace |
| spelling | upm.eprints-173102015-11-23T04:18:28Z http://psasir.upm.edu.my/id/eprint/17310/ The resin-embedded cornea prepared via rapid processing protocol : a good histomorphometric target for clinical investigation in opthalmology and optometry. Cheah , Pike See Mohidin, Norhani Mohd Ali, Bariah Maung , Myint Abdul Latif , Azian This study illustrates and quantifies the changes on corneal tissue between the paraffin-embedded and resin-embedded blocks and thus, selects a better target in investigational ophthalmology and optometry via light microscopy. Corneas of two cynomolgus monkeys (Macaca fascicularis) were used in this study. The formalin-fixed cornea was prepared in paraffin block via the conventional tissue processing protocol (4-day protocol) and stained with haematoxylin and eosin. The glutaraldehyde-fixed cornea was prepared in resin block via the rapid and modified tissue processing procedure (1.2-day protocol) and stained with toluidine blue. The paraffin-embedded sample exhibits various undesired tissue damage and artifact such as thinner epithelium (due to the substantial volumic extraction from the tissue), thicker stroma layer (due to the separation of lamellae and the presence of voids) and the distorted endothelium. In contrast, the resin-embedded corneal tissue has demonstrated satisfactory corneal ultrastructural preservation. The rapid and modified tissue processing method for preparing the resin-embedded is particularly beneficial to accelerate the microscopic evaluation in ophthalmology and optometry. Penerbit Usm 2008-07 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/17310/1/The%20resin.pdf Cheah , Pike See and Mohidin, Norhani and Mohd Ali, Bariah and Maung , Myint and Abdul Latif , Azian (2008) The resin-embedded cornea prepared via rapid processing protocol : a good histomorphometric target for clinical investigation in opthalmology and optometry. Malaysian Journal of Medical Sciences, 15 (3). pp. 49-54. ISSN 1394-195X, ESSN: 2180-4303 Cornea. Cornea — Measurement. Ophthalmology. English |
| spellingShingle | Cornea. Cornea — Measurement. Ophthalmology. Cheah , Pike See Mohidin, Norhani Mohd Ali, Bariah Maung , Myint Abdul Latif , Azian The resin-embedded cornea prepared via rapid processing protocol : a good histomorphometric target for clinical investigation in opthalmology and optometry. |
| title | The resin-embedded cornea prepared via rapid processing protocol : a good histomorphometric target for clinical investigation in opthalmology and optometry. |
| title_full | The resin-embedded cornea prepared via rapid processing protocol : a good histomorphometric target for clinical investigation in opthalmology and optometry. |
| title_fullStr | The resin-embedded cornea prepared via rapid processing protocol : a good histomorphometric target for clinical investigation in opthalmology and optometry. |
| title_full_unstemmed | The resin-embedded cornea prepared via rapid processing protocol : a good histomorphometric target for clinical investigation in opthalmology and optometry. |
| title_short | The resin-embedded cornea prepared via rapid processing protocol : a good histomorphometric target for clinical investigation in opthalmology and optometry. |
| title_sort | resin embedded cornea prepared via rapid processing protocol a good histomorphometric target for clinical investigation in opthalmology and optometry |
| topic | Cornea. Cornea — Measurement. Ophthalmology. |
| url | http://psasir.upm.edu.my/id/eprint/17310/1/The%20resin.pdf |
| work_keys_str_mv | AT cheahpikesee theresinembeddedcorneapreparedviarapidprocessingprotocolagoodhistomorphometrictargetforclinicalinvestigationinopthalmologyandoptometry AT mohidinnorhani theresinembeddedcorneapreparedviarapidprocessingprotocolagoodhistomorphometrictargetforclinicalinvestigationinopthalmologyandoptometry AT mohdalibariah theresinembeddedcorneapreparedviarapidprocessingprotocolagoodhistomorphometrictargetforclinicalinvestigationinopthalmologyandoptometry AT maungmyint theresinembeddedcorneapreparedviarapidprocessingprotocolagoodhistomorphometrictargetforclinicalinvestigationinopthalmologyandoptometry AT abdullatifazian theresinembeddedcorneapreparedviarapidprocessingprotocolagoodhistomorphometrictargetforclinicalinvestigationinopthalmologyandoptometry AT cheahpikesee resinembeddedcorneapreparedviarapidprocessingprotocolagoodhistomorphometrictargetforclinicalinvestigationinopthalmologyandoptometry AT mohidinnorhani resinembeddedcorneapreparedviarapidprocessingprotocolagoodhistomorphometrictargetforclinicalinvestigationinopthalmologyandoptometry AT mohdalibariah resinembeddedcorneapreparedviarapidprocessingprotocolagoodhistomorphometrictargetforclinicalinvestigationinopthalmologyandoptometry AT maungmyint resinembeddedcorneapreparedviarapidprocessingprotocolagoodhistomorphometrictargetforclinicalinvestigationinopthalmologyandoptometry AT abdullatifazian resinembeddedcorneapreparedviarapidprocessingprotocolagoodhistomorphometrictargetforclinicalinvestigationinopthalmologyandoptometry |