The structural differences of ewes oviductal ampulla secretory cells during follicular and luteal phases - a scanning and transmission electron microscopical investigation
The aim of the present study was to differentiate the ultrastructure as features of secretory cells within the ampulla region of the sheep oviduct during follicular and luteal phases of estrous cycle. Fourteen ewes were slaughtered either at the peak of follicular or luteal phase for sample collecti...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Electron Microscopy Society Malaysia
2008
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/17346/1/The%20structural%20differences%20of%20ewes%20oviductal%20ampulla%20secretory%20cells%20during%20follicular%20and%20luteal%20phases%20-%20a%20scanning%20and%20transmission%20electron%20microscopical%20investigation.pdf |
| _version_ | 1796969237379547136 |
|---|---|
| author | Abdul Razak, Intan Shameha Abu Bakar @ Zakaria, Md Zuki Tengku Ibrahim, Tengku Azmi Haron, Abd Wahid Mohamed Mustapha, Noordin |
| author_facet | Abdul Razak, Intan Shameha Abu Bakar @ Zakaria, Md Zuki Tengku Ibrahim, Tengku Azmi Haron, Abd Wahid Mohamed Mustapha, Noordin |
| author_sort | Abdul Razak, Intan Shameha |
| collection | UPM |
| description | The aim of the present study was to differentiate the ultrastructure as features of secretory cells within the ampulla region of the sheep oviduct during follicular and luteal phases of estrous cycle. Fourteen ewes were slaughtered either at the peak of follicular or luteal phase for sample collection. Ampulla were taken and processed accordingly for scanning and transmission electron microscopy. Blood samples were taken every alternate day for hormonal profiles analysis using RIA. During follicular phase, the population of the secretory cells was lower as compared to the luteal phase, while the number of ciliated cells was higher. The secretory cells were rounded, turgid with intact microvilli but during luteal phase the surfaces were broken and some secretions were oozing out. From TEM, at follicular phase, the secretory cells have blunt processes at the apex with intact microvilli, but during luteal phase, the cytoplasmic protrusion increased in volume. Numerous secretory granules were identified and obser ed in both phases but they were of different sizes and electron density. In conclusion, the present observations revealed marked cyclic changes and differences of the secretory cells during these two phases of estrous cycle. During follicular phase, the secretory cells were at the preparatory stage while they were actively secreting during luteal phase. |
| first_indexed | 2024-03-06T07:40:09Z |
| format | Article |
| id | upm.eprints-17346 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T07:40:09Z |
| publishDate | 2008 |
| publisher | Electron Microscopy Society Malaysia |
| record_format | dspace |
| spelling | upm.eprints-173462016-11-23T04:36:56Z http://psasir.upm.edu.my/id/eprint/17346/ The structural differences of ewes oviductal ampulla secretory cells during follicular and luteal phases - a scanning and transmission electron microscopical investigation Abdul Razak, Intan Shameha Abu Bakar @ Zakaria, Md Zuki Tengku Ibrahim, Tengku Azmi Haron, Abd Wahid Mohamed Mustapha, Noordin The aim of the present study was to differentiate the ultrastructure as features of secretory cells within the ampulla region of the sheep oviduct during follicular and luteal phases of estrous cycle. Fourteen ewes were slaughtered either at the peak of follicular or luteal phase for sample collection. Ampulla were taken and processed accordingly for scanning and transmission electron microscopy. Blood samples were taken every alternate day for hormonal profiles analysis using RIA. During follicular phase, the population of the secretory cells was lower as compared to the luteal phase, while the number of ciliated cells was higher. The secretory cells were rounded, turgid with intact microvilli but during luteal phase the surfaces were broken and some secretions were oozing out. From TEM, at follicular phase, the secretory cells have blunt processes at the apex with intact microvilli, but during luteal phase, the cytoplasmic protrusion increased in volume. Numerous secretory granules were identified and obser ed in both phases but they were of different sizes and electron density. In conclusion, the present observations revealed marked cyclic changes and differences of the secretory cells during these two phases of estrous cycle. During follicular phase, the secretory cells were at the preparatory stage while they were actively secreting during luteal phase. Electron Microscopy Society Malaysia 2008 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/17346/1/The%20structural%20differences%20of%20ewes%20oviductal%20ampulla%20secretory%20cells%20during%20follicular%20and%20luteal%20phases%20-%20a%20scanning%20and%20transmission%20electron%20microscopical%20investigation.pdf Abdul Razak, Intan Shameha and Abu Bakar @ Zakaria, Md Zuki and Tengku Ibrahim, Tengku Azmi and Haron, Abd Wahid and Mohamed Mustapha, Noordin (2008) The structural differences of ewes oviductal ampulla secretory cells during follicular and luteal phases - a scanning and transmission electron microscopical investigation. Malaysian Journal of Microscopy, 4. pp. 7-11. ISSN 1823-7010 |
| spellingShingle | Abdul Razak, Intan Shameha Abu Bakar @ Zakaria, Md Zuki Tengku Ibrahim, Tengku Azmi Haron, Abd Wahid Mohamed Mustapha, Noordin The structural differences of ewes oviductal ampulla secretory cells during follicular and luteal phases - a scanning and transmission electron microscopical investigation |
| title | The structural differences of ewes oviductal ampulla secretory cells during follicular and luteal phases - a scanning and transmission electron microscopical investigation |
| title_full | The structural differences of ewes oviductal ampulla secretory cells during follicular and luteal phases - a scanning and transmission electron microscopical investigation |
| title_fullStr | The structural differences of ewes oviductal ampulla secretory cells during follicular and luteal phases - a scanning and transmission electron microscopical investigation |
| title_full_unstemmed | The structural differences of ewes oviductal ampulla secretory cells during follicular and luteal phases - a scanning and transmission electron microscopical investigation |
| title_short | The structural differences of ewes oviductal ampulla secretory cells during follicular and luteal phases - a scanning and transmission electron microscopical investigation |
| title_sort | structural differences of ewes oviductal ampulla secretory cells during follicular and luteal phases a scanning and transmission electron microscopical investigation |
| url | http://psasir.upm.edu.my/id/eprint/17346/1/The%20structural%20differences%20of%20ewes%20oviductal%20ampulla%20secretory%20cells%20during%20follicular%20and%20luteal%20phases%20-%20a%20scanning%20and%20transmission%20electron%20microscopical%20investigation.pdf |
| work_keys_str_mv | AT abdulrazakintanshameha thestructuraldifferencesofewesoviductalampullasecretorycellsduringfollicularandlutealphasesascanningandtransmissionelectronmicroscopicalinvestigation AT abubakarzakariamdzuki thestructuraldifferencesofewesoviductalampullasecretorycellsduringfollicularandlutealphasesascanningandtransmissionelectronmicroscopicalinvestigation AT tengkuibrahimtengkuazmi thestructuraldifferencesofewesoviductalampullasecretorycellsduringfollicularandlutealphasesascanningandtransmissionelectronmicroscopicalinvestigation AT haronabdwahid thestructuraldifferencesofewesoviductalampullasecretorycellsduringfollicularandlutealphasesascanningandtransmissionelectronmicroscopicalinvestigation AT mohamedmustaphanoordin thestructuraldifferencesofewesoviductalampullasecretorycellsduringfollicularandlutealphasesascanningandtransmissionelectronmicroscopicalinvestigation AT abdulrazakintanshameha structuraldifferencesofewesoviductalampullasecretorycellsduringfollicularandlutealphasesascanningandtransmissionelectronmicroscopicalinvestigation AT abubakarzakariamdzuki structuraldifferencesofewesoviductalampullasecretorycellsduringfollicularandlutealphasesascanningandtransmissionelectronmicroscopicalinvestigation AT tengkuibrahimtengkuazmi structuraldifferencesofewesoviductalampullasecretorycellsduringfollicularandlutealphasesascanningandtransmissionelectronmicroscopicalinvestigation AT haronabdwahid structuraldifferencesofewesoviductalampullasecretorycellsduringfollicularandlutealphasesascanningandtransmissionelectronmicroscopicalinvestigation AT mohamedmustaphanoordin structuraldifferencesofewesoviductalampullasecretorycellsduringfollicularandlutealphasesascanningandtransmissionelectronmicroscopicalinvestigation |