In vitro activity of fluconazole and voriconazole against clinical isolates of Candida spp. by E-test method.
The in vitro susceptibility of clinical Candida isolates towards fluconazole and voriconazole was determined using the E-test method. A total of 41 clinical isolates recovered from patients since 2004 until 2009 from two local hospitals in Kuala Lumpur, Malaysia were used. These comprised Candida tr...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English English |
| Published: |
CAB Direct
2010
|
| Subjects: | |
| Online Access: | http://psasir.upm.edu.my/id/eprint/17714/1/In%20vitro%20activity%20of%20fluconazole%20and%20voriconazole%20against%20clinical%20isolates%20of%20Candida%20spp.pdf |
| _version_ | 1825946064359784448 |
|---|---|
| author | Jamal, Farida Fatema @ Farida Madhavan, Priya Chong, Pei Pei Ng, Kee Peng |
| author_facet | Jamal, Farida Fatema @ Farida Madhavan, Priya Chong, Pei Pei Ng, Kee Peng |
| author_sort | Jamal, Farida Fatema @ Farida |
| collection | UPM |
| description | The in vitro susceptibility of clinical Candida isolates towards fluconazole and voriconazole was determined using the E-test method. A total of 41 clinical isolates recovered from patients since 2004 until 2009 from two local hospitals in Kuala Lumpur, Malaysia were used. These comprised Candida tropicalis, Candida albicans, Candida krusei, Candida parapsilosis, Candida rugosa, Candida dubliniensis and Candida glabrata. Strains from American Type Culture Collection were used as quality control. Lawn cultures of the isolates on RPMI-1640 agar medium supplemented with 2% glucose were incubated with the E-test strips at 35°C for 48 h. Our results show that 71% were susceptible to fluconazole and 90% were susceptible to voriconazole. All strains of C. krusei were resistant to fluconazole and 50% were susceptible in a dose-dependent manner to voriconazole. There were 66% and 33% of C. glabrata that were resistant to fluconazole and voriconazole. Our study revealed that majority of the clinical Candida isolates was susceptible to fluconazole and voriconazole with a small percentage being resistant to both the drugs.
|
| first_indexed | 2024-03-06T07:41:15Z |
| format | Article |
| id | upm.eprints-17714 |
| institution | Universiti Putra Malaysia |
| language | English English |
| last_indexed | 2024-03-06T07:41:15Z |
| publishDate | 2010 |
| publisher | CAB Direct |
| record_format | dspace |
| spelling | upm.eprints-177142015-10-08T01:57:36Z http://psasir.upm.edu.my/id/eprint/17714/ In vitro activity of fluconazole and voriconazole against clinical isolates of Candida spp. by E-test method. Jamal, Farida Fatema @ Farida Madhavan, Priya Chong, Pei Pei Ng, Kee Peng The in vitro susceptibility of clinical Candida isolates towards fluconazole and voriconazole was determined using the E-test method. A total of 41 clinical isolates recovered from patients since 2004 until 2009 from two local hospitals in Kuala Lumpur, Malaysia were used. These comprised Candida tropicalis, Candida albicans, Candida krusei, Candida parapsilosis, Candida rugosa, Candida dubliniensis and Candida glabrata. Strains from American Type Culture Collection were used as quality control. Lawn cultures of the isolates on RPMI-1640 agar medium supplemented with 2% glucose were incubated with the E-test strips at 35°C for 48 h. Our results show that 71% were susceptible to fluconazole and 90% were susceptible to voriconazole. All strains of C. krusei were resistant to fluconazole and 50% were susceptible in a dose-dependent manner to voriconazole. There were 66% and 33% of C. glabrata that were resistant to fluconazole and voriconazole. Our study revealed that majority of the clinical Candida isolates was susceptible to fluconazole and voriconazole with a small percentage being resistant to both the drugs. CAB Direct 2010 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/17714/1/In%20vitro%20activity%20of%20fluconazole%20and%20voriconazole%20against%20clinical%20isolates%20of%20Candida%20spp.pdf Jamal, Farida Fatema @ Farida and Madhavan, Priya and Chong, Pei Pei and Ng, Kee Peng (2010) In vitro activity of fluconazole and voriconazole against clinical isolates of Candida spp. by E-test method. Tropical Biomedicine, 27 (2). pp. 200-207. ISSN 0127-5720 http://www.cabi.org Candida Drug - Resistance Drug - Susceptibility English |
| spellingShingle | Candida Drug - Resistance Drug - Susceptibility Jamal, Farida Fatema @ Farida Madhavan, Priya Chong, Pei Pei Ng, Kee Peng In vitro activity of fluconazole and voriconazole against clinical isolates of Candida spp. by E-test method. |
| title | In vitro activity of fluconazole and voriconazole against
clinical isolates of Candida spp. by E-test method. |
| title_full | In vitro activity of fluconazole and voriconazole against
clinical isolates of Candida spp. by E-test method. |
| title_fullStr | In vitro activity of fluconazole and voriconazole against
clinical isolates of Candida spp. by E-test method. |
| title_full_unstemmed | In vitro activity of fluconazole and voriconazole against
clinical isolates of Candida spp. by E-test method. |
| title_short | In vitro activity of fluconazole and voriconazole against
clinical isolates of Candida spp. by E-test method. |
| title_sort | in vitro activity of fluconazole and voriconazole against clinical isolates of candida spp by e test method |
| topic | Candida Drug - Resistance Drug - Susceptibility |
| url | http://psasir.upm.edu.my/id/eprint/17714/1/In%20vitro%20activity%20of%20fluconazole%20and%20voriconazole%20against%20clinical%20isolates%20of%20Candida%20spp.pdf |
| work_keys_str_mv | AT jamalfaridafatemafarida invitroactivityoffluconazoleandvoriconazoleagainstclinicalisolatesofcandidasppbyetestmethod AT madhavanpriya invitroactivityoffluconazoleandvoriconazoleagainstclinicalisolatesofcandidasppbyetestmethod AT chongpeipei invitroactivityoffluconazoleandvoriconazoleagainstclinicalisolatesofcandidasppbyetestmethod AT ngkeepeng invitroactivityoffluconazoleandvoriconazoleagainstclinicalisolatesofcandidasppbyetestmethod |