Effect of preservation methods on the performance of bovine pericardium graft in a rat model

This study investigates the effect of preservation methods on the performance of bovine parietal pericardium grafts in a rat model. Mid-ventral full thickness abdominal wall defects of 3x2.5 cm in size were created in 90 male Sprague-Dawley rats (300-400 g), which were divided into three groups of 3...

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Main Authors: Abu Bakar @ Zakaria, Md Zuki, Yagoub Mohamed, Abdel Hafeez, Mohamad Yusof, Loqman, Mohamed Mustapha, Noordin, Yusof, Norimah
Format: Article
Language:English
Published: Blackwell Verlag GmbH 2007
Online Access:http://psasir.upm.edu.my/id/eprint/17951/1/Effect%20of%20preservation%20methods%20on%20the%20performance%20of%20bovine%20pericardium%20graft%20in%20a%20rat%20model.pdf
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author Abu Bakar @ Zakaria, Md Zuki
Yagoub Mohamed, Abdel Hafeez
Mohamad Yusof, Loqman
Mohamed Mustapha, Noordin
Yusof, Norimah
author_facet Abu Bakar @ Zakaria, Md Zuki
Yagoub Mohamed, Abdel Hafeez
Mohamad Yusof, Loqman
Mohamed Mustapha, Noordin
Yusof, Norimah
author_sort Abu Bakar @ Zakaria, Md Zuki
collection UPM
description This study investigates the effect of preservation methods on the performance of bovine parietal pericardium grafts in a rat model. Mid-ventral full thickness abdominal wall defects of 3x2.5 cm in size were created in 90 male Sprague-Dawley rats (300-400 g), which were divided into three groups of 30 rats each. The abdominal defects of group one and two were repaired with lyophilized and glycerolized bovine pericardium grafts, while the defects of group three were repaired with expanded polytetrafluoroethylene (ePTFE) Mycro Mesh as a positive control. Another group of 30 rats underwent sham operation and was used for comparison as negative control. Each group of rats (n=30) was divided into five subgroups (n=6) and killed at 1, 3, 6, 9 and 18 weeks post-surgery for gross and morphological evaluations. The rats tolerated the surgical procedure well with a total mortality of 0.05%. No serious post-operative clinical complications or signs of rejection were encountered. Adhesions between the grafts and the underlying visceral organs observed in the study were mostly results of post-surgical complications. Glycerol preservation delayed degradation and replacement of the grafts, whereas lyophilization caused early resorption and replacement of the grafts. The glycerolized grafts were replaced with thick dense fibrous tissue, and the lyophilized grafts were replaced with thin loose fibrous tissue. The healing characteristic of the bovine pericardium grafts was similar to those of the sham-operated group, and quite different from those of the ePTFE Mycro Mesh. The outcome of the present study confirmed the superiority of glycerolized bovine pericardium grafts over its lyophilized counter part.
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spelling upm.eprints-179512016-11-21T01:36:15Z http://psasir.upm.edu.my/id/eprint/17951/ Effect of preservation methods on the performance of bovine pericardium graft in a rat model Abu Bakar @ Zakaria, Md Zuki Yagoub Mohamed, Abdel Hafeez Mohamad Yusof, Loqman Mohamed Mustapha, Noordin Yusof, Norimah This study investigates the effect of preservation methods on the performance of bovine parietal pericardium grafts in a rat model. Mid-ventral full thickness abdominal wall defects of 3x2.5 cm in size were created in 90 male Sprague-Dawley rats (300-400 g), which were divided into three groups of 30 rats each. The abdominal defects of group one and two were repaired with lyophilized and glycerolized bovine pericardium grafts, while the defects of group three were repaired with expanded polytetrafluoroethylene (ePTFE) Mycro Mesh as a positive control. Another group of 30 rats underwent sham operation and was used for comparison as negative control. Each group of rats (n=30) was divided into five subgroups (n=6) and killed at 1, 3, 6, 9 and 18 weeks post-surgery for gross and morphological evaluations. The rats tolerated the surgical procedure well with a total mortality of 0.05%. No serious post-operative clinical complications or signs of rejection were encountered. Adhesions between the grafts and the underlying visceral organs observed in the study were mostly results of post-surgical complications. Glycerol preservation delayed degradation and replacement of the grafts, whereas lyophilization caused early resorption and replacement of the grafts. The glycerolized grafts were replaced with thick dense fibrous tissue, and the lyophilized grafts were replaced with thin loose fibrous tissue. The healing characteristic of the bovine pericardium grafts was similar to those of the sham-operated group, and quite different from those of the ePTFE Mycro Mesh. The outcome of the present study confirmed the superiority of glycerolized bovine pericardium grafts over its lyophilized counter part. Blackwell Verlag GmbH 2007-10 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/17951/1/Effect%20of%20preservation%20methods%20on%20the%20performance%20of%20bovine%20pericardium%20graft%20in%20a%20rat%20model.pdf Abu Bakar @ Zakaria, Md Zuki and Yagoub Mohamed, Abdel Hafeez and Mohamad Yusof, Loqman and Mohamed Mustapha, Noordin and Yusof, Norimah (2007) Effect of preservation methods on the performance of bovine pericardium graft in a rat model. Anatomia, Histologia, Embryologia, 36 (5). pp. 349-356. ISSN 0340-2096; ESSN: 1439-0264 10.1111/j.1439-0264.2007.00772.x
spellingShingle Abu Bakar @ Zakaria, Md Zuki
Yagoub Mohamed, Abdel Hafeez
Mohamad Yusof, Loqman
Mohamed Mustapha, Noordin
Yusof, Norimah
Effect of preservation methods on the performance of bovine pericardium graft in a rat model
title Effect of preservation methods on the performance of bovine pericardium graft in a rat model
title_full Effect of preservation methods on the performance of bovine pericardium graft in a rat model
title_fullStr Effect of preservation methods on the performance of bovine pericardium graft in a rat model
title_full_unstemmed Effect of preservation methods on the performance of bovine pericardium graft in a rat model
title_short Effect of preservation methods on the performance of bovine pericardium graft in a rat model
title_sort effect of preservation methods on the performance of bovine pericardium graft in a rat model
url http://psasir.upm.edu.my/id/eprint/17951/1/Effect%20of%20preservation%20methods%20on%20the%20performance%20of%20bovine%20pericardium%20graft%20in%20a%20rat%20model.pdf
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AT mohamedmustaphanoordin effectofpreservationmethodsontheperformanceofbovinepericardiumgraftinaratmodel
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