Genetic relatedness among isolates of Acanthamoeba based on RAPD analysis
Acanthamoeba is a pathogen frequently infecting brain, eyes, skin and lung of human and animal. Seven Acanthamoeba local isolates from various sources, two Acanthamoeba type strains and one strain of Hartmannella vermiformis were characterized for their genetic variability using randomly amplified p...
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| Format: | Article |
| Language: | English English |
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2006
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| Online Access: | http://psasir.upm.edu.my/id/eprint/18312/1/Genetic%20relatedness%20among%20isolates%20of%20Acanthamoeba%20based%20on%20RAPD%20analysis.pdf |
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| author | Abdul Manaf, Siti Ruhaya Mustafa, Shuhaimi Mat Amin, Nakisah Ali, Abdul Manaf |
| author_facet | Abdul Manaf, Siti Ruhaya Mustafa, Shuhaimi Mat Amin, Nakisah Ali, Abdul Manaf |
| author_sort | Abdul Manaf, Siti Ruhaya |
| collection | UPM |
| description | Acanthamoeba is a pathogen frequently infecting brain, eyes, skin and lung of human and animal. Seven Acanthamoeba local isolates from various sources, two Acanthamoeba type strains and one strain of Hartmannella vermiformis were characterized for their genetic variability using randomly amplified polymorphic DNA (RAPD) technique using four different 10-mer oligonucleotides primers. Electrophoresis of the amplification products generated DNA bands ranging from approximately 0.25 to 7.50 kbp in size. A genetic relatedness among the isolates was examined using Dice similarity coefficient as the genetic distance measured between the strains of Acanthamoeba and H. vermiformis. Three distinct clusters could be separated at genetic distance of approximately 0.330. © 2006 Asian Network for Scientific Information. |
| first_indexed | 2024-03-06T07:42:52Z |
| format | Article |
| id | upm.eprints-18312 |
| institution | Universiti Putra Malaysia |
| language | English English |
| last_indexed | 2024-03-06T07:42:52Z |
| publishDate | 2006 |
| record_format | dspace |
| spelling | upm.eprints-183122015-12-09T02:57:51Z http://psasir.upm.edu.my/id/eprint/18312/ Genetic relatedness among isolates of Acanthamoeba based on RAPD analysis Abdul Manaf, Siti Ruhaya Mustafa, Shuhaimi Mat Amin, Nakisah Ali, Abdul Manaf Acanthamoeba is a pathogen frequently infecting brain, eyes, skin and lung of human and animal. Seven Acanthamoeba local isolates from various sources, two Acanthamoeba type strains and one strain of Hartmannella vermiformis were characterized for their genetic variability using randomly amplified polymorphic DNA (RAPD) technique using four different 10-mer oligonucleotides primers. Electrophoresis of the amplification products generated DNA bands ranging from approximately 0.25 to 7.50 kbp in size. A genetic relatedness among the isolates was examined using Dice similarity coefficient as the genetic distance measured between the strains of Acanthamoeba and H. vermiformis. Three distinct clusters could be separated at genetic distance of approximately 0.330. © 2006 Asian Network for Scientific Information. 2006 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/18312/1/Genetic%20relatedness%20among%20isolates%20of%20Acanthamoeba%20based%20on%20RAPD%20analysis.pdf Abdul Manaf, Siti Ruhaya and Mustafa, Shuhaimi and Mat Amin, Nakisah and Ali, Abdul Manaf (2006) Genetic relatedness among isolates of Acanthamoeba based on RAPD analysis. Journal of Applied Sciences, 6 (1). pp. 15-19. ISSN 1812-5654 http://dx.doi.org/10.3923/jas.2006.15.19 Acanthamoeba - Malaysia Genetic polymorphisms doi:10.3923/jas.2006.15.19 English |
| spellingShingle | Acanthamoeba - Malaysia Genetic polymorphisms Abdul Manaf, Siti Ruhaya Mustafa, Shuhaimi Mat Amin, Nakisah Ali, Abdul Manaf Genetic relatedness among isolates of Acanthamoeba based on RAPD analysis |
| title | Genetic relatedness among isolates of Acanthamoeba based on RAPD analysis |
| title_full | Genetic relatedness among isolates of Acanthamoeba based on RAPD analysis |
| title_fullStr | Genetic relatedness among isolates of Acanthamoeba based on RAPD analysis |
| title_full_unstemmed | Genetic relatedness among isolates of Acanthamoeba based on RAPD analysis |
| title_short | Genetic relatedness among isolates of Acanthamoeba based on RAPD analysis |
| title_sort | genetic relatedness among isolates of acanthamoeba based on rapd analysis |
| topic | Acanthamoeba - Malaysia Genetic polymorphisms |
| url | http://psasir.upm.edu.my/id/eprint/18312/1/Genetic%20relatedness%20among%20isolates%20of%20Acanthamoeba%20based%20on%20RAPD%20analysis.pdf |
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