Establishment of Physalis minima hairy roots culture for the production of physalins
This report describes the technique used to induce the hairy roots in Physalis minima (Linn.). Different types of explants obtained from in vitro germinated seedlings were aseptically co-cultivated with A. rhizogenes strain LBA9402 in different media. Root growth and production of physalins were inv...
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| Format: | Article |
| Language: | English English English |
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Springer
2002
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| Online Access: | http://psasir.upm.edu.my/id/eprint/18376/1/Establishment%20of%20Physalis%20minima%20hairy%20roots%20culture%20for%20the%20production%20of%20physalins.pdf http://psasir.upm.edu.my/id/eprint/18376/7/A_1015662118877.pdf |
| _version_ | 1825946181241405440 |
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| author | Ganzau, Jualang Azlan Mahmood, Maziah Muse, Radzali Ramli, Johari |
| author_facet | Ganzau, Jualang Azlan Mahmood, Maziah Muse, Radzali Ramli, Johari |
| author_sort | Ganzau, Jualang Azlan |
| collection | UPM |
| description | This report describes the technique used to induce the hairy roots in Physalis minima (Linn.). Different types of explants obtained from in vitro germinated seedlings were aseptically co-cultivated with A. rhizogenes strain LBA9402 in different media. Root growth and production of physalins were investigated in various basal media grown under dark and light conditions, and compared to that of normal root cultures. Transformed hairy root cultures grew rapidly and reach stationary phase after 15 days on a B5 medium. HPLC analysis of extracts of hairy root cultures showed that the maximum content of physalin B and F was 1.82 and 4.15 mg g-1 DW, respectively, when grown under dark conditions. Normal root cultures produced higher physalin B (1.60-1.62 mg g-1 DW) and F (3.30-3.75 mg g-1 DW) under the same culture conditions. Physalin F synthesis in light-grown root cultures was reduced significantly. |
| first_indexed | 2024-03-06T07:43:02Z |
| format | Article |
| id | upm.eprints-18376 |
| institution | Universiti Putra Malaysia |
| language | English English English |
| last_indexed | 2024-09-25T03:33:12Z |
| publishDate | 2002 |
| publisher | Springer |
| record_format | dspace |
| spelling | upm.eprints-183762024-08-05T07:10:40Z http://psasir.upm.edu.my/id/eprint/18376/ Establishment of Physalis minima hairy roots culture for the production of physalins Ganzau, Jualang Azlan Mahmood, Maziah Muse, Radzali Ramli, Johari This report describes the technique used to induce the hairy roots in Physalis minima (Linn.). Different types of explants obtained from in vitro germinated seedlings were aseptically co-cultivated with A. rhizogenes strain LBA9402 in different media. Root growth and production of physalins were investigated in various basal media grown under dark and light conditions, and compared to that of normal root cultures. Transformed hairy root cultures grew rapidly and reach stationary phase after 15 days on a B5 medium. HPLC analysis of extracts of hairy root cultures showed that the maximum content of physalin B and F was 1.82 and 4.15 mg g-1 DW, respectively, when grown under dark conditions. Normal root cultures produced higher physalin B (1.60-1.62 mg g-1 DW) and F (3.30-3.75 mg g-1 DW) under the same culture conditions. Physalin F synthesis in light-grown root cultures was reduced significantly. Springer 2002 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/18376/1/Establishment%20of%20Physalis%20minima%20hairy%20roots%20culture%20for%20the%20production%20of%20physalins.pdf text en http://psasir.upm.edu.my/id/eprint/18376/7/A_1015662118877.pdf Ganzau, Jualang Azlan and Mahmood, Maziah and Muse, Radzali and Ramli, Johari (2002) Establishment of Physalis minima hairy roots culture for the production of physalins. Plant Cell, Tissue and Organ Culture, 69 (3). pp. 271-278. ISSN 0167-6857 http://dx.doi.org/10.1023/A:1015662118877 Physalis - Genetics Plant genetic transformation Plant metabolites doi:10.1023/A:1015662118877 English |
| spellingShingle | Physalis - Genetics Plant genetic transformation Plant metabolites Ganzau, Jualang Azlan Mahmood, Maziah Muse, Radzali Ramli, Johari Establishment of Physalis minima hairy roots culture for the production of physalins |
| title | Establishment of Physalis minima hairy roots culture for the production of physalins |
| title_full | Establishment of Physalis minima hairy roots culture for the production of physalins |
| title_fullStr | Establishment of Physalis minima hairy roots culture for the production of physalins |
| title_full_unstemmed | Establishment of Physalis minima hairy roots culture for the production of physalins |
| title_short | Establishment of Physalis minima hairy roots culture for the production of physalins |
| title_sort | establishment of physalis minima hairy roots culture for the production of physalins |
| topic | Physalis - Genetics Plant genetic transformation Plant metabolites |
| url | http://psasir.upm.edu.my/id/eprint/18376/1/Establishment%20of%20Physalis%20minima%20hairy%20roots%20culture%20for%20the%20production%20of%20physalins.pdf http://psasir.upm.edu.my/id/eprint/18376/7/A_1015662118877.pdf |
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