Growth and anthraquinone production of Morinda elliptica cell suspension cultures in a stirred-tank bioreactor
The effects of medium strategy, number of impellers, aeration mode, and mode of operation on Morinda elliptica cell suspension cultures in a stirred-tank bioreactor are described. A lower number of impellers and continuous aeration contributed toward high cell growth rate, whereas a higher number of...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English English |
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ACS Publications
2000
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| Online Access: | http://psasir.upm.edu.my/id/eprint/18377/1/Growth%20and%20anthraquinone%20production%20of%20Morinda%20elliptica%20cell%20suspension%20cultures%20in%20a%20stirred.pdf |
| _version_ | 1796969442473672704 |
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| author | Abdullah, Mohd Azmuddin Ariff, Arbakariya Mahmood, Maziah Ali, Abdul Manaf Lajis, Md Nordin |
| author_facet | Abdullah, Mohd Azmuddin Ariff, Arbakariya Mahmood, Maziah Ali, Abdul Manaf Lajis, Md Nordin |
| author_sort | Abdullah, Mohd Azmuddin |
| collection | UPM |
| description | The effects of medium strategy, number of impellers, aeration mode, and mode of operation on Morinda elliptica cell suspension cultures in a stirred-tank bioreactor are described. A lower number of impellers and continuous aeration contributed toward high cell growth rate, whereas a higher number of impellers reduced cell growth rate, although not anthraquinone yield. The semicontinuous mode could indirectly imitate the larger scale version of production medium strategy and improved anthraquinone production even with 0.012% (v/v) antifoam addition. Production medium promoted both growth (maximum dry cell weight of 24.6 g/L) and anthraquinone formation (maximum content of 19.5 mg/g of dry cell weight), without any necessity for antifoam addition. Cultures in production medium or with higher growth rate and anthraquinone production were less acidic than cultures in growth medium or with lower growth rate and anthraquinone production. Using the best operating variables, growth of M. elliptica cells (24.6 g/L) and anthraquinone yield (0.25 g/L) were 45% and 140%, respectively, lower than those using a shake flask culture after 12 days of cultivation. |
| first_indexed | 2024-03-06T07:43:03Z |
| format | Article |
| id | upm.eprints-18377 |
| institution | Universiti Putra Malaysia |
| language | English English |
| last_indexed | 2024-03-06T07:43:03Z |
| publishDate | 2000 |
| publisher | ACS Publications |
| record_format | dspace |
| spelling | upm.eprints-183772015-12-09T08:31:48Z http://psasir.upm.edu.my/id/eprint/18377/ Growth and anthraquinone production of Morinda elliptica cell suspension cultures in a stirred-tank bioreactor Abdullah, Mohd Azmuddin Ariff, Arbakariya Mahmood, Maziah Ali, Abdul Manaf Lajis, Md Nordin The effects of medium strategy, number of impellers, aeration mode, and mode of operation on Morinda elliptica cell suspension cultures in a stirred-tank bioreactor are described. A lower number of impellers and continuous aeration contributed toward high cell growth rate, whereas a higher number of impellers reduced cell growth rate, although not anthraquinone yield. The semicontinuous mode could indirectly imitate the larger scale version of production medium strategy and improved anthraquinone production even with 0.012% (v/v) antifoam addition. Production medium promoted both growth (maximum dry cell weight of 24.6 g/L) and anthraquinone formation (maximum content of 19.5 mg/g of dry cell weight), without any necessity for antifoam addition. Cultures in production medium or with higher growth rate and anthraquinone production were less acidic than cultures in growth medium or with lower growth rate and anthraquinone production. Using the best operating variables, growth of M. elliptica cells (24.6 g/L) and anthraquinone yield (0.25 g/L) were 45% and 140%, respectively, lower than those using a shake flask culture after 12 days of cultivation. ACS Publications 2000 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/18377/1/Growth%20and%20anthraquinone%20production%20of%20Morinda%20elliptica%20cell%20suspension%20cultures%20in%20a%20stirred.pdf Abdullah, Mohd Azmuddin and Ariff, Arbakariya and Mahmood, Maziah and Ali, Abdul Manaf and Lajis, Md Nordin (2000) Growth and anthraquinone production of Morinda elliptica cell suspension cultures in a stirred-tank bioreactor. Journal of Agricultural and Food Chemistry, 48 (9). pp. 4432-4438. ISSN 0021-8561 http://dx.doi.org/10.1021/jf991014v Anthraquinones Morinda - Biotechnology - Malaysia Plant tissue culture doi:10.1021/jf991014v English |
| spellingShingle | Anthraquinones Morinda - Biotechnology - Malaysia Plant tissue culture Abdullah, Mohd Azmuddin Ariff, Arbakariya Mahmood, Maziah Ali, Abdul Manaf Lajis, Md Nordin Growth and anthraquinone production of Morinda elliptica cell suspension cultures in a stirred-tank bioreactor |
| title | Growth and anthraquinone production of Morinda elliptica cell suspension cultures in a stirred-tank bioreactor |
| title_full | Growth and anthraquinone production of Morinda elliptica cell suspension cultures in a stirred-tank bioreactor |
| title_fullStr | Growth and anthraquinone production of Morinda elliptica cell suspension cultures in a stirred-tank bioreactor |
| title_full_unstemmed | Growth and anthraquinone production of Morinda elliptica cell suspension cultures in a stirred-tank bioreactor |
| title_short | Growth and anthraquinone production of Morinda elliptica cell suspension cultures in a stirred-tank bioreactor |
| title_sort | growth and anthraquinone production of morinda elliptica cell suspension cultures in a stirred tank bioreactor |
| topic | Anthraquinones Morinda - Biotechnology - Malaysia Plant tissue culture |
| url | http://psasir.upm.edu.my/id/eprint/18377/1/Growth%20and%20anthraquinone%20production%20of%20Morinda%20elliptica%20cell%20suspension%20cultures%20in%20a%20stirred.pdf |
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