Changes in pH of legume cell suspension culture media containing aluminum.
When different levels of aluminum was added to the L-6 liquid media there was a marked decrease in pH values. The wide pH shift obtained indicated that pH adjustments prior to the addition of aluminum were essential in order to standardise the initial pH of all treatments at 4.0 ± 0.1. The pH ad...
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| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
1990
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| Online Access: | http://psasir.upm.edu.my/id/eprint/18653/1/ID%2018653.pdf |
| _version_ | 1825946228095975424 |
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| author | Mahmood, Marziah |
| author_facet | Mahmood, Marziah |
| author_sort | Mahmood, Marziah |
| collection | UPM |
| description | When different levels of aluminum was added to the L-6 liquid
media there was a marked decrease in pH values. The wide pH
shift obtained indicated that pH adjustments prior to the
addition of aluminum were essential in order to standardise the
initial pH of all treatments at 4.0 ± 0.1. The pH adjustments
made to the L-6 liquid media prior to autoclaving for treatments
0, 50, 100, 200, 400, 600, and 800 uM Al were 4.4, 5.1, 5.6, 5.9,
6.1, 6.6, and 7.6, respectively. When the respective levels of
Al were added the pH of the media of each treatment was found to
be about 4.0 ± 0.1. Further examinations showed that the pH of
the culture media containing tepary bean cell suspensions and
whose initial pH was 4.5 increased gradually to about 5.0 within
12 days of culture. The pH of the culture media remained around
5.0 regardless of the initial pH values (4.5 and 5.3). However,
the pH appeared to be relatively constant at 4.0 ± 0.1 for five
days. This suggested that the cultures had to be transferred to
a fresh medium every 4-5 days to maintain the pH of the
media at 4.0 ± 0.1 and to maximise the toxic effects of aluminum. |
| first_indexed | 2024-03-06T07:43:46Z |
| format | Conference or Workshop Item |
| id | upm.eprints-18653 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T07:43:46Z |
| publishDate | 1990 |
| record_format | dspace |
| spelling | upm.eprints-186532015-04-13T04:19:04Z http://psasir.upm.edu.my/id/eprint/18653/ Changes in pH of legume cell suspension culture media containing aluminum. Mahmood, Marziah When different levels of aluminum was added to the L-6 liquid media there was a marked decrease in pH values. The wide pH shift obtained indicated that pH adjustments prior to the addition of aluminum were essential in order to standardise the initial pH of all treatments at 4.0 ± 0.1. The pH adjustments made to the L-6 liquid media prior to autoclaving for treatments 0, 50, 100, 200, 400, 600, and 800 uM Al were 4.4, 5.1, 5.6, 5.9, 6.1, 6.6, and 7.6, respectively. When the respective levels of Al were added the pH of the media of each treatment was found to be about 4.0 ± 0.1. Further examinations showed that the pH of the culture media containing tepary bean cell suspensions and whose initial pH was 4.5 increased gradually to about 5.0 within 12 days of culture. The pH of the culture media remained around 5.0 regardless of the initial pH values (4.5 and 5.3). However, the pH appeared to be relatively constant at 4.0 ± 0.1 for five days. This suggested that the cultures had to be transferred to a fresh medium every 4-5 days to maintain the pH of the media at 4.0 ± 0.1 and to maximise the toxic effects of aluminum. 1990 Conference or Workshop Item NonPeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/18653/1/ID%2018653.pdf Mahmood, Marziah (1990) Changes in pH of legume cell suspension culture media containing aluminum. In: International Symposium Plant Soil Interaction at Low pH, 24-29 June 1990, Beckley, USA. . Legumes Plants |
| spellingShingle | Legumes Plants Mahmood, Marziah Changes in pH of legume cell suspension culture media containing aluminum. |
| title | Changes in pH of legume cell suspension culture media containing aluminum. |
| title_full | Changes in pH of legume cell suspension culture media containing aluminum. |
| title_fullStr | Changes in pH of legume cell suspension culture media containing aluminum. |
| title_full_unstemmed | Changes in pH of legume cell suspension culture media containing aluminum. |
| title_short | Changes in pH of legume cell suspension culture media containing aluminum. |
| title_sort | changes in ph of legume cell suspension culture media containing aluminum |
| topic | Legumes Plants |
| url | http://psasir.upm.edu.my/id/eprint/18653/1/ID%2018653.pdf |
| work_keys_str_mv | AT mahmoodmarziah changesinphoflegumecellsuspensionculturemediacontainingaluminum |