Extractability of soluble protein from different types of skeletal muscles in cattle under the treatment of pH, molarity and type of buffers
Soluble protein can be extracted if muscle fibres are lysed and the extractability depends on several factors such as choice of buffer, presence of protein inhibitor and the osmolarity of the resuspension buffer (1). Proteins can be severely affected by small changes in hydrogen ion concentration. S...
| Main Authors: | , , , , |
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| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
Halal Products Research Institute, Universiti Putra Malaysia
2009
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| Online Access: | http://psasir.upm.edu.my/id/eprint/19891/1/73-6.pdf |
| _version_ | 1796969697582776320 |
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| author | M. Z., Noor Azihan Sazili, Awis Qurni Mustafa, Shuhaimi Abdullah, Muhammad Hilmi Panandam, Jothi Malar |
| author_facet | M. Z., Noor Azihan Sazili, Awis Qurni Mustafa, Shuhaimi Abdullah, Muhammad Hilmi Panandam, Jothi Malar |
| author_sort | M. Z., Noor Azihan |
| collection | UPM |
| description | Soluble protein can be extracted if muscle fibres are lysed and the extractability depends on several factors such as choice of buffer, presence of protein inhibitor and the osmolarity of the resuspension buffer (1). Proteins can be severely affected by small changes in hydrogen ion concentration. Some proteins tend to precipitate when the pH approaches their isoelectric point (pI) (7). Therefore, in order to ensure reproducible results, it is important to maintain the protein solution at the constant pH. Once an optimal pH has been determined, different buffer within the
same pH range may also be examined for specific buffer effects (2). Different types of skeletal muscles are composed of different proportion of fibers at which the differences influence the metabolic capability (4, 6). Differences in rate of post-mortem glycolysis could induce changes in protein solubility (3). In addition, the selection of the ST, LD and SS muscles for the study
was based on their previously reported metabolite and contractile characteristic as well as fiber type composition (5). The aim of this study was to evaluate the effects of the type, pH and molarity of extraction buffer on protein extractability from beef Semitendinosus (ST) Longissimus dorsi (LD) and Supraspinatus (SS) muscles. |
| first_indexed | 2024-03-06T07:46:52Z |
| format | Conference or Workshop Item |
| id | upm.eprints-19891 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T07:46:52Z |
| publishDate | 2009 |
| publisher | Halal Products Research Institute, Universiti Putra Malaysia |
| record_format | dspace |
| spelling | upm.eprints-198912018-08-03T01:20:47Z http://psasir.upm.edu.my/id/eprint/19891/ Extractability of soluble protein from different types of skeletal muscles in cattle under the treatment of pH, molarity and type of buffers M. Z., Noor Azihan Sazili, Awis Qurni Mustafa, Shuhaimi Abdullah, Muhammad Hilmi Panandam, Jothi Malar Soluble protein can be extracted if muscle fibres are lysed and the extractability depends on several factors such as choice of buffer, presence of protein inhibitor and the osmolarity of the resuspension buffer (1). Proteins can be severely affected by small changes in hydrogen ion concentration. Some proteins tend to precipitate when the pH approaches their isoelectric point (pI) (7). Therefore, in order to ensure reproducible results, it is important to maintain the protein solution at the constant pH. Once an optimal pH has been determined, different buffer within the same pH range may also be examined for specific buffer effects (2). Different types of skeletal muscles are composed of different proportion of fibers at which the differences influence the metabolic capability (4, 6). Differences in rate of post-mortem glycolysis could induce changes in protein solubility (3). In addition, the selection of the ST, LD and SS muscles for the study was based on their previously reported metabolite and contractile characteristic as well as fiber type composition (5). The aim of this study was to evaluate the effects of the type, pH and molarity of extraction buffer on protein extractability from beef Semitendinosus (ST) Longissimus dorsi (LD) and Supraspinatus (SS) muscles. Halal Products Research Institute, Universiti Putra Malaysia 2009 Conference or Workshop Item PeerReviewed text en http://psasir.upm.edu.my/id/eprint/19891/1/73-6.pdf M. Z., Noor Azihan and Sazili, Awis Qurni and Mustafa, Shuhaimi and Abdullah, Muhammad Hilmi and Panandam, Jothi Malar (2009) Extractability of soluble protein from different types of skeletal muscles in cattle under the treatment of pH, molarity and type of buffers. In: 3rd IMT-GT International Symposium on Halal science and Management 2009, 21-22 Dec. 2009, KLIA Sepang, Malaysia. (pp. 29-31). |
| spellingShingle | M. Z., Noor Azihan Sazili, Awis Qurni Mustafa, Shuhaimi Abdullah, Muhammad Hilmi Panandam, Jothi Malar Extractability of soluble protein from different types of skeletal muscles in cattle under the treatment of pH, molarity and type of buffers |
| title | Extractability of soluble protein from different types of skeletal muscles in cattle under the treatment of pH, molarity and type of buffers |
| title_full | Extractability of soluble protein from different types of skeletal muscles in cattle under the treatment of pH, molarity and type of buffers |
| title_fullStr | Extractability of soluble protein from different types of skeletal muscles in cattle under the treatment of pH, molarity and type of buffers |
| title_full_unstemmed | Extractability of soluble protein from different types of skeletal muscles in cattle under the treatment of pH, molarity and type of buffers |
| title_short | Extractability of soluble protein from different types of skeletal muscles in cattle under the treatment of pH, molarity and type of buffers |
| title_sort | extractability of soluble protein from different types of skeletal muscles in cattle under the treatment of ph molarity and type of buffers |
| url | http://psasir.upm.edu.my/id/eprint/19891/1/73-6.pdf |
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