Method for producing a recombinant thermostable geobacillus T1 lipase
The present invention provides a method for producing a recombinant thermostable Geobacillus T1 lipase comprising (a) expression of the thermostable Geobacillus T1 lipase without the signal peptide (amino acids) as a GST-fusion protein in a prokaryotic host strain, and (b) affinity chromatography pu...
Main Authors: | , , , |
---|---|
Format: | Patent |
Published: |
2008
|
_version_ | 1825946514197839872 |
---|---|
author | Raja Abdul Rahman, Raja Noor Zaliha Salleh, Abu Bakar Basri, Mahiran Leow, Thean Chor |
author_facet | Raja Abdul Rahman, Raja Noor Zaliha Salleh, Abu Bakar Basri, Mahiran Leow, Thean Chor |
author_sort | Raja Abdul Rahman, Raja Noor Zaliha |
collection | UPM |
description | The present invention provides a method for producing a recombinant thermostable Geobacillus T1 lipase comprising (a) expression of the thermostable Geobacillus T1 lipase without the signal peptide (amino acids) as a GST-fusion protein in a prokaryotic host strain, and (b) affinity chromatography purification of said GST-fusion protein. |
first_indexed | 2024-03-06T07:48:22Z |
format | Patent |
id | upm.eprints-20390 |
institution | Universiti Putra Malaysia |
last_indexed | 2024-03-06T07:48:22Z |
publishDate | 2008 |
record_format | dspace |
spelling | upm.eprints-203902016-09-27T02:09:07Z http://psasir.upm.edu.my/id/eprint/20390/ Method for producing a recombinant thermostable geobacillus T1 lipase Raja Abdul Rahman, Raja Noor Zaliha Salleh, Abu Bakar Basri, Mahiran Leow, Thean Chor The present invention provides a method for producing a recombinant thermostable Geobacillus T1 lipase comprising (a) expression of the thermostable Geobacillus T1 lipase without the signal peptide (amino acids) as a GST-fusion protein in a prokaryotic host strain, and (b) affinity chromatography purification of said GST-fusion protein. 2008-11-19 Patent NonPeerReviewed Raja Noor Zaliha Raja Abd. Rahman (2008) Method for producing a recombinant thermostable geobacillus T1 lipase. UNSPECIFIED. |
spellingShingle | Raja Abdul Rahman, Raja Noor Zaliha Salleh, Abu Bakar Basri, Mahiran Leow, Thean Chor Method for producing a recombinant thermostable geobacillus T1 lipase |
title | Method for producing a recombinant thermostable geobacillus T1 lipase |
title_full | Method for producing a recombinant thermostable geobacillus T1 lipase |
title_fullStr | Method for producing a recombinant thermostable geobacillus T1 lipase |
title_full_unstemmed | Method for producing a recombinant thermostable geobacillus T1 lipase |
title_short | Method for producing a recombinant thermostable geobacillus T1 lipase |
title_sort | method for producing a recombinant thermostable geobacillus t1 lipase |
work_keys_str_mv | AT rajaabdulrahmanrajanoorzaliha methodforproducingarecombinantthermostablegeobacillust1lipase AT sallehabubakar methodforproducingarecombinantthermostablegeobacillust1lipase AT basrimahiran methodforproducingarecombinantthermostablegeobacillust1lipase AT leowtheanchor methodforproducingarecombinantthermostablegeobacillust1lipase |