Detection of Newcastle disease virus.
A method for detection of Newcastle disease virus in a biological sample comprising (a) the extraction of mRNA from the sample (b) converting the mRNA from step (a) with reverse transcriptase under conditions allowing for the production of cDNA; ? amplifying the cDNA from step (b) by polymerase chai...
| Main Authors: | , , , |
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| Format: | Patent |
| Published: |
2007
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| _version_ | 1796969872278683648 |
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| author | Yusoff, Khatijah Kho, Chiew Ling Mohd Lila, Mohd Azmi Arshad, Siti Suri |
| author_facet | Yusoff, Khatijah Kho, Chiew Ling Mohd Lila, Mohd Azmi Arshad, Siti Suri |
| author_sort | Yusoff, Khatijah |
| collection | UPM |
| description | A method for detection of Newcastle disease virus in a biological sample comprising (a) the extraction of mRNA from the sample (b) converting the mRNA from step (a) with reverse transcriptase under conditions allowing for the production of cDNA; ? amplifying the cDNA from step (b) by polymerase chain reaction to form an amplified PCR product (d) converting the amplified PCR product from step ? with a pair of nested primers to form a nested labelled PCR streptavidin-coated microtiter plate; (f) adding a peroxidase substrate to the immobilised product from step (e) to produce a coloured reaction product; and (g) detecting the resulting coloured reaction product using an ELISA reader.
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| first_indexed | 2024-03-06T07:49:35Z |
| format | Patent |
| id | upm.eprints-20884 |
| institution | Universiti Putra Malaysia |
| last_indexed | 2024-03-06T07:49:35Z |
| publishDate | 2007 |
| record_format | dspace |
| spelling | upm.eprints-208842013-06-14T02:50:27Z http://psasir.upm.edu.my/id/eprint/20884/ Detection of Newcastle disease virus. Yusoff, Khatijah Kho, Chiew Ling Mohd Lila, Mohd Azmi Arshad, Siti Suri A method for detection of Newcastle disease virus in a biological sample comprising (a) the extraction of mRNA from the sample (b) converting the mRNA from step (a) with reverse transcriptase under conditions allowing for the production of cDNA; ? amplifying the cDNA from step (b) by polymerase chain reaction to form an amplified PCR product (d) converting the amplified PCR product from step ? with a pair of nested primers to form a nested labelled PCR streptavidin-coated microtiter plate; (f) adding a peroxidase substrate to the immobilised product from step (e) to produce a coloured reaction product; and (g) detecting the resulting coloured reaction product using an ELISA reader. 2007-12-31 Patent NonPeerReviewed Khatijah Yusoff (2007) Detection of Newcastle disease virus. UNSPECIFIED. |
| spellingShingle | Yusoff, Khatijah Kho, Chiew Ling Mohd Lila, Mohd Azmi Arshad, Siti Suri Detection of Newcastle disease virus. |
| title | Detection of Newcastle disease virus.
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| title_full | Detection of Newcastle disease virus.
|
| title_fullStr | Detection of Newcastle disease virus.
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| title_full_unstemmed | Detection of Newcastle disease virus.
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| title_short | Detection of Newcastle disease virus.
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| title_sort | detection of newcastle disease virus |
| work_keys_str_mv | AT yusoffkhatijah detectionofnewcastlediseasevirus AT khochiewling detectionofnewcastlediseasevirus AT mohdlilamohdazmi detectionofnewcastlediseasevirus AT arshadsitisuri detectionofnewcastlediseasevirus |