Nitrification of high-strength ammonium landfill leachate with microbial community analysis using (FISH).
Nitrification of mature sanitary landfill leachate with high-strength of N_NHþ 4 (1080–2350mg L_1) was performed in a 10 L continuous nitrification activated sludge reactor. The nitrification system was acclimatized with synthetic leachate during feed batch operation to avoid substrate inhibition be...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English English |
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Sage Publication
2011
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| Online Access: | http://psasir.upm.edu.my/id/eprint/22243/1/Nitrification%20of%20high-strength%20ammonium%20landfill%20leachate%20with%20microbial%20community%20analysis%20using%20%28FISH%29..pdf |
| _version_ | 1796970122207821824 |
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| author | Yusof, Norjan Hassan, Mohd Ali Phang, Lai Yee Tabatabaei, Meisam Othman, Mohd Ridzuan Mori, Masatsugu Wakisaka, Minato Sakai, Kenji Shirai, Yoshihito |
| author_facet | Yusof, Norjan Hassan, Mohd Ali Phang, Lai Yee Tabatabaei, Meisam Othman, Mohd Ridzuan Mori, Masatsugu Wakisaka, Minato Sakai, Kenji Shirai, Yoshihito |
| author_sort | Yusof, Norjan |
| collection | UPM |
| description | Nitrification of mature sanitary landfill leachate with high-strength of N_NHþ 4 (1080–2350mg L_1) was performed in a 10 L continuous nitrification activated sludge reactor. The nitrification system was acclimatized with synthetic leachate during feed batch operation to avoid substrate inhibition before being fed with actual mature leachate. Successful nitrification was achieved with an approximately complete ammonium removal (99%) and 96% of N_NHþ
4 conversion to N_NO_3 . The maximum volumetric and specific nitrification rates obtained were 2.56 kg N_NHþ4 m_3 day_1 and 0.23 g N_NHþ4g_1 volatile suspended solid (VSS) day_1, respectively, at hydraulic retention time (HRT) of 12.7 h and solid retention time of 50 days. Incomplete nitrification was encountered when operating at a higher nitrogen loading rate of 3.14 kg N_NHþ 4 m_3 day_1. The substrate overloading and nitrifiers competition with heterotrophs were believed to trigger the incomplete nitrification. Fluorescence in situ hybridization (FISH) results supported the syntrophic association between
the ammonia-oxidizing bacteria (AOB) and nitrite-oxidizing bacteria. FISH results also revealed the heterotrophs as the dominant and disintegration of some AOB cell aggregates into single cells which further supported the incomplete nitrification phenomenon.
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| first_indexed | 2024-03-06T07:53:22Z |
| format | Article |
| id | upm.eprints-22243 |
| institution | Universiti Putra Malaysia |
| language | English English |
| last_indexed | 2024-03-06T07:53:22Z |
| publishDate | 2011 |
| publisher | Sage Publication |
| record_format | dspace |
| spelling | upm.eprints-222432015-10-28T04:33:36Z http://psasir.upm.edu.my/id/eprint/22243/ Nitrification of high-strength ammonium landfill leachate with microbial community analysis using (FISH). Yusof, Norjan Hassan, Mohd Ali Phang, Lai Yee Tabatabaei, Meisam Othman, Mohd Ridzuan Mori, Masatsugu Wakisaka, Minato Sakai, Kenji Shirai, Yoshihito Nitrification of mature sanitary landfill leachate with high-strength of N_NHþ 4 (1080–2350mg L_1) was performed in a 10 L continuous nitrification activated sludge reactor. The nitrification system was acclimatized with synthetic leachate during feed batch operation to avoid substrate inhibition before being fed with actual mature leachate. Successful nitrification was achieved with an approximately complete ammonium removal (99%) and 96% of N_NHþ 4 conversion to N_NO_3 . The maximum volumetric and specific nitrification rates obtained were 2.56 kg N_NHþ4 m_3 day_1 and 0.23 g N_NHþ4g_1 volatile suspended solid (VSS) day_1, respectively, at hydraulic retention time (HRT) of 12.7 h and solid retention time of 50 days. Incomplete nitrification was encountered when operating at a higher nitrogen loading rate of 3.14 kg N_NHþ 4 m_3 day_1. The substrate overloading and nitrifiers competition with heterotrophs were believed to trigger the incomplete nitrification. Fluorescence in situ hybridization (FISH) results supported the syntrophic association between the ammonia-oxidizing bacteria (AOB) and nitrite-oxidizing bacteria. FISH results also revealed the heterotrophs as the dominant and disintegration of some AOB cell aggregates into single cells which further supported the incomplete nitrification phenomenon. Sage Publication 2011 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/22243/1/Nitrification%20of%20high-strength%20ammonium%20landfill%20leachate%20with%20microbial%20community%20analysis%20using%20%28FISH%29..pdf Yusof, Norjan and Hassan, Mohd Ali and Phang, Lai Yee and Tabatabaei, Meisam and Othman, Mohd Ridzuan and Mori, Masatsugu and Wakisaka, Minato and Sakai, Kenji and Shirai, Yoshihito (2011) Nitrification of high-strength ammonium landfill leachate with microbial community analysis using (FISH). Waste Management and Research, 29 (6). pp. 602-611. ISSN 0734-242X 10.1177/0734242X10397581 English |
| spellingShingle | Yusof, Norjan Hassan, Mohd Ali Phang, Lai Yee Tabatabaei, Meisam Othman, Mohd Ridzuan Mori, Masatsugu Wakisaka, Minato Sakai, Kenji Shirai, Yoshihito Nitrification of high-strength ammonium landfill leachate with microbial community analysis using (FISH). |
| title | Nitrification of high-strength ammonium landfill leachate with microbial community analysis using (FISH). |
| title_full | Nitrification of high-strength ammonium landfill leachate with microbial community analysis using (FISH). |
| title_fullStr | Nitrification of high-strength ammonium landfill leachate with microbial community analysis using (FISH). |
| title_full_unstemmed | Nitrification of high-strength ammonium landfill leachate with microbial community analysis using (FISH). |
| title_short | Nitrification of high-strength ammonium landfill leachate with microbial community analysis using (FISH). |
| title_sort | nitrification of high strength ammonium landfill leachate with microbial community analysis using fish |
| url | http://psasir.upm.edu.my/id/eprint/22243/1/Nitrification%20of%20high-strength%20ammonium%20landfill%20leachate%20with%20microbial%20community%20analysis%20using%20%28FISH%29..pdf |
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