Crystallization and preliminary X-ray crystallographic analysis of a thermostable organic solvent-tolerant lipase from Bacillus sp. strain 42
An organic solvent-tolerant lipase from Bacillus sp. strain 42 was crystallized using the capillary-tube method. The purpose of studying this enzyme was in order to better understand its folding and to characterize its properties in organic solvents. By initially solving its structure in the native...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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International Union of Crystallography
2011
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| Online Access: | http://psasir.upm.edu.my/id/eprint/22266/1/Crystallization%20and%20preliminary%20X.pdf |
| _version_ | 1825946839219699712 |
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| author | Khusaini, Mohd Saif Raja Abdul Rahman, Raja Noor Zaliha Mohamad Ali, Mohd Shukuri Leow, Adam Thean Chor Basri, Mahiran Salleh, Abu Bakar |
| author_facet | Khusaini, Mohd Saif Raja Abdul Rahman, Raja Noor Zaliha Mohamad Ali, Mohd Shukuri Leow, Adam Thean Chor Basri, Mahiran Salleh, Abu Bakar |
| author_sort | Khusaini, Mohd Saif |
| collection | UPM |
| description | An organic solvent-tolerant lipase from Bacillus sp. strain 42 was crystallized using the capillary-tube method. The purpose of studying this enzyme was in order to better understand its folding and to characterize its properties in organic solvents. By initially solving its structure in the native state, further studies on protein-solvent interactions could be performed. X-ray data were collected at 2.0 Å resolution using an in-house diffractometer. The estimated crystal dimensions were 0.09 0.19 0.08 mm. The crystal belonged to the monoclinic space group C2, with unit-cell parameters a = 117.41, b = 80.85, c = 99.44 Å, β = 96.40°. |
| first_indexed | 2024-03-06T07:53:27Z |
| format | Article |
| id | upm.eprints-22266 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T07:53:27Z |
| publishDate | 2011 |
| publisher | International Union of Crystallography |
| record_format | dspace |
| spelling | upm.eprints-222662016-09-28T01:48:05Z http://psasir.upm.edu.my/id/eprint/22266/ Crystallization and preliminary X-ray crystallographic analysis of a thermostable organic solvent-tolerant lipase from Bacillus sp. strain 42 Khusaini, Mohd Saif Raja Abdul Rahman, Raja Noor Zaliha Mohamad Ali, Mohd Shukuri Leow, Adam Thean Chor Basri, Mahiran Salleh, Abu Bakar An organic solvent-tolerant lipase from Bacillus sp. strain 42 was crystallized using the capillary-tube method. The purpose of studying this enzyme was in order to better understand its folding and to characterize its properties in organic solvents. By initially solving its structure in the native state, further studies on protein-solvent interactions could be performed. X-ray data were collected at 2.0 Å resolution using an in-house diffractometer. The estimated crystal dimensions were 0.09 0.19 0.08 mm. The crystal belonged to the monoclinic space group C2, with unit-cell parameters a = 117.41, b = 80.85, c = 99.44 Å, β = 96.40°. International Union of Crystallography 2011 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/22266/1/Crystallization%20and%20preliminary%20X.pdf Khusaini, Mohd Saif and Raja Abdul Rahman, Raja Noor Zaliha and Mohamad Ali, Mohd Shukuri and Leow, Adam Thean Chor and Basri, Mahiran and Salleh, Abu Bakar (2011) Crystallization and preliminary X-ray crystallographic analysis of a thermostable organic solvent-tolerant lipase from Bacillus sp. strain 42. Acta Crystallographica Section F: Structural Biology and Crystallization Communications, 67 (3). pp. 401-403. ISSN 1744-3091 http://scripts.iucr.org/cgi-bin/paper?S1744309111002028 10.1107/S1744309111002028 |
| spellingShingle | Khusaini, Mohd Saif Raja Abdul Rahman, Raja Noor Zaliha Mohamad Ali, Mohd Shukuri Leow, Adam Thean Chor Basri, Mahiran Salleh, Abu Bakar Crystallization and preliminary X-ray crystallographic analysis of a thermostable organic solvent-tolerant lipase from Bacillus sp. strain 42 |
| title | Crystallization and preliminary X-ray crystallographic analysis of a thermostable organic solvent-tolerant lipase from Bacillus sp. strain 42 |
| title_full | Crystallization and preliminary X-ray crystallographic analysis of a thermostable organic solvent-tolerant lipase from Bacillus sp. strain 42 |
| title_fullStr | Crystallization and preliminary X-ray crystallographic analysis of a thermostable organic solvent-tolerant lipase from Bacillus sp. strain 42 |
| title_full_unstemmed | Crystallization and preliminary X-ray crystallographic analysis of a thermostable organic solvent-tolerant lipase from Bacillus sp. strain 42 |
| title_short | Crystallization and preliminary X-ray crystallographic analysis of a thermostable organic solvent-tolerant lipase from Bacillus sp. strain 42 |
| title_sort | crystallization and preliminary x ray crystallographic analysis of a thermostable organic solvent tolerant lipase from bacillus sp strain 42 |
| url | http://psasir.upm.edu.my/id/eprint/22266/1/Crystallization%20and%20preliminary%20X.pdf |
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