Novel polymorphic microsatellite DNA markers from Malaysian giant freshwater prawn, Macrobrachium rosenbergii.
Seven single locus microsatellite markers were characterized in Malaysian giant freshwater prawn, Macrobrachium rosenbergii from an enriched genomic library Primer pairs were designed to flank the repeat sequences and the loci characterized for this species. The bands resulting from the PCR amplific...
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Format: | Article |
Language: | English |
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Springer
2011
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author | See, Leng Min Hassan, Rosly Tan, Soon Guan Bhassu, Subha |
author_facet | See, Leng Min Hassan, Rosly Tan, Soon Guan Bhassu, Subha |
author_sort | See, Leng Min |
collection | UPM |
description | Seven single locus microsatellite markers were characterized in Malaysian giant freshwater prawn, Macrobrachium rosenbergii from an enriched genomic library Primer pairs were designed to flank the repeat sequences and the loci characterized for this species. The bands resulting from the PCR amplifications of these eight microsatellite loci were polymorphic with the number of alleles ranging from 8 to 26 alleles per locus, whereas the observed heterozygosity ranged from 0.0641 to 0.6564. These newly developed microsatellite markers should prove to be useful for population studies and in the management of genetic variations in broodstocks of freshwater prawn, M. rosenbergii. |
first_indexed | 2024-03-06T07:53:28Z |
format | Article |
id | upm.eprints-22272 |
institution | Universiti Putra Malaysia |
language | English |
last_indexed | 2024-03-06T07:53:28Z |
publishDate | 2011 |
publisher | Springer |
record_format | dspace |
spelling | upm.eprints-222722014-10-13T07:10:50Z http://psasir.upm.edu.my/id/eprint/22272/ Novel polymorphic microsatellite DNA markers from Malaysian giant freshwater prawn, Macrobrachium rosenbergii. See, Leng Min Hassan, Rosly Tan, Soon Guan Bhassu, Subha Seven single locus microsatellite markers were characterized in Malaysian giant freshwater prawn, Macrobrachium rosenbergii from an enriched genomic library Primer pairs were designed to flank the repeat sequences and the loci characterized for this species. The bands resulting from the PCR amplifications of these eight microsatellite loci were polymorphic with the number of alleles ranging from 8 to 26 alleles per locus, whereas the observed heterozygosity ranged from 0.0641 to 0.6564. These newly developed microsatellite markers should prove to be useful for population studies and in the management of genetic variations in broodstocks of freshwater prawn, M. rosenbergii. Springer 2011 Article PeerReviewed See, Leng Min and Hassan, Rosly and Tan, Soon Guan and Bhassu, Subha (2011) Novel polymorphic microsatellite DNA markers from Malaysian giant freshwater prawn, Macrobrachium rosenbergii. Russian Journal of Genetics, 47 (4). pp. 497-499. ISSN 1022-7954 10.1134/S102279541103015X English |
spellingShingle | See, Leng Min Hassan, Rosly Tan, Soon Guan Bhassu, Subha Novel polymorphic microsatellite DNA markers from Malaysian giant freshwater prawn, Macrobrachium rosenbergii. |
title | Novel polymorphic microsatellite DNA markers from Malaysian giant freshwater prawn, Macrobrachium rosenbergii. |
title_full | Novel polymorphic microsatellite DNA markers from Malaysian giant freshwater prawn, Macrobrachium rosenbergii. |
title_fullStr | Novel polymorphic microsatellite DNA markers from Malaysian giant freshwater prawn, Macrobrachium rosenbergii. |
title_full_unstemmed | Novel polymorphic microsatellite DNA markers from Malaysian giant freshwater prawn, Macrobrachium rosenbergii. |
title_short | Novel polymorphic microsatellite DNA markers from Malaysian giant freshwater prawn, Macrobrachium rosenbergii. |
title_sort | novel polymorphic microsatellite dna markers from malaysian giant freshwater prawn macrobrachium rosenbergii |
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