Partial protection against enterovirus 71 (EV71) infection in a mouse model immunized with recombinant newcastle disease virus capsids displaying the EV71 VP1 fragment.
Enterovirus 71 (EV71) infection may cause severe neurological complications, particularly in young children. Despite the risks, there are still no commercially available EV71 vaccines. Hence, a candidate vaccine construct, containing recombinant Newcastle disease virus capsids that display an EV71 V...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English English |
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Wiley-Blackwell
2011
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| Online Access: | http://psasir.upm.edu.my/id/eprint/22285/1/Partial%20protection%20against%20enterovirus%2071.pdf |
| _version_ | 1825946842930610176 |
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| author | Wei, Choong Ch'ng Stanbridge, Eric J. Kien, Chai Ong Kum, Thong Wong Yusoff, Khatijah Shafee , Norazizah |
| author_facet | Wei, Choong Ch'ng Stanbridge, Eric J. Kien, Chai Ong Kum, Thong Wong Yusoff, Khatijah Shafee , Norazizah |
| author_sort | Wei, Choong Ch'ng |
| collection | UPM |
| description | Enterovirus 71 (EV71) infection may cause severe neurological complications, particularly in young children. Despite the risks, there are still no commercially available EV71 vaccines. Hence, a candidate vaccine construct, containing recombinant Newcastle disease virus capsids that display an EV71 VP1 fragment (NPt-VP1 1-100) protein, was evaluated in a mouse model of EV71 infection. Previously, it was shown that this protein construct provoked a strong immune response in vaccinated adult rabbits. That study, however, did not address the issue of its effectiveness against EV71 infection in young animals. In the present study, EV71 viral challenge in vaccinated newborn mice resulted in more than 40% increase in survival rate. Significantly, half of the surviving mice fully recovered from their paralysis. Histological analysis of all of the surviving mice revealed a complete clearance of EV71 viral antigens from their brains and spinal cords. In hind limb muscles, the amounts of the antigens detected correlated with the degrees of tissue damage and paralysis. Findings from this study provide evidence that immunization with the NPt-VP1 1-100 immunogen in a newborn mouse model confers partial protection against EV71 infection, and also highlights the importance of NPt-VP1 1-100 as a possible candidate vaccine for protection against EV71 infections. |
| first_indexed | 2024-03-06T07:53:30Z |
| format | Article |
| id | upm.eprints-22285 |
| institution | Universiti Putra Malaysia |
| language | English English |
| last_indexed | 2024-03-06T07:53:30Z |
| publishDate | 2011 |
| publisher | Wiley-Blackwell |
| record_format | dspace |
| spelling | upm.eprints-222852015-10-09T07:42:10Z http://psasir.upm.edu.my/id/eprint/22285/ Partial protection against enterovirus 71 (EV71) infection in a mouse model immunized with recombinant newcastle disease virus capsids displaying the EV71 VP1 fragment. Wei, Choong Ch'ng Stanbridge, Eric J. Kien, Chai Ong Kum, Thong Wong Yusoff, Khatijah Shafee , Norazizah Enterovirus 71 (EV71) infection may cause severe neurological complications, particularly in young children. Despite the risks, there are still no commercially available EV71 vaccines. Hence, a candidate vaccine construct, containing recombinant Newcastle disease virus capsids that display an EV71 VP1 fragment (NPt-VP1 1-100) protein, was evaluated in a mouse model of EV71 infection. Previously, it was shown that this protein construct provoked a strong immune response in vaccinated adult rabbits. That study, however, did not address the issue of its effectiveness against EV71 infection in young animals. In the present study, EV71 viral challenge in vaccinated newborn mice resulted in more than 40% increase in survival rate. Significantly, half of the surviving mice fully recovered from their paralysis. Histological analysis of all of the surviving mice revealed a complete clearance of EV71 viral antigens from their brains and spinal cords. In hind limb muscles, the amounts of the antigens detected correlated with the degrees of tissue damage and paralysis. Findings from this study provide evidence that immunization with the NPt-VP1 1-100 immunogen in a newborn mouse model confers partial protection against EV71 infection, and also highlights the importance of NPt-VP1 1-100 as a possible candidate vaccine for protection against EV71 infections. Wiley-Blackwell 2011-10 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/22285/1/Partial%20protection%20against%20enterovirus%2071.pdf Wei, Choong Ch'ng and Stanbridge, Eric J. and Kien, Chai Ong and Kum, Thong Wong and Yusoff, Khatijah and Shafee , Norazizah (2011) Partial protection against enterovirus 71 (EV71) infection in a mouse model immunized with recombinant newcastle disease virus capsids displaying the EV71 VP1 fragment. Journal of Medical Virology, 83 (10). pp. 1783-1791. ISSN 0146-6615, ESSN: 1096-9071 http://www.wiley.com/wiley-blackwell/ 10.1002/jmv.22198 English |
| spellingShingle | Wei, Choong Ch'ng Stanbridge, Eric J. Kien, Chai Ong Kum, Thong Wong Yusoff, Khatijah Shafee , Norazizah Partial protection against enterovirus 71 (EV71) infection in a mouse model immunized with recombinant newcastle disease virus capsids displaying the EV71 VP1 fragment. |
| title | Partial protection against enterovirus 71 (EV71) infection in a mouse model immunized with recombinant newcastle disease virus capsids displaying the EV71 VP1 fragment. |
| title_full | Partial protection against enterovirus 71 (EV71) infection in a mouse model immunized with recombinant newcastle disease virus capsids displaying the EV71 VP1 fragment. |
| title_fullStr | Partial protection against enterovirus 71 (EV71) infection in a mouse model immunized with recombinant newcastle disease virus capsids displaying the EV71 VP1 fragment. |
| title_full_unstemmed | Partial protection against enterovirus 71 (EV71) infection in a mouse model immunized with recombinant newcastle disease virus capsids displaying the EV71 VP1 fragment. |
| title_short | Partial protection against enterovirus 71 (EV71) infection in a mouse model immunized with recombinant newcastle disease virus capsids displaying the EV71 VP1 fragment. |
| title_sort | partial protection against enterovirus 71 ev71 infection in a mouse model immunized with recombinant newcastle disease virus capsids displaying the ev71 vp1 fragment |
| url | http://psasir.upm.edu.my/id/eprint/22285/1/Partial%20protection%20against%20enterovirus%2071.pdf |
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