Primary capture of cyclodextrin glycosyltransferase derived from Bacillus cereus by aqueous two phase system.
In this works, the polymer–salt aqueous two phase system (ATPS) which is polyethylene–glycol (PEG) with sodium citrate was constructed to purify the enzyme CGTase from fermentation broth. Impacts of parameters such as phase composition, tie-line length (TLL), volume ratio (VR), crude sample loading,...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English English |
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Elsevier
2011
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| Online Access: | http://psasir.upm.edu.my/id/eprint/22298/1/Primary%20capture%20of%20cyclodextrin%20glycosyltransferase%20derived%20from%20Bacillus%20cereus%20by%20aqueous%20two%20phase%20system.pdf |
| _version_ | 1825946845488087040 |
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| author | Ng, Hui Suan Tan, Chin Ping Chen, Soo Kien Mokhtar, Mohd Noriznan Ariff, Arbakariya Ling, Tau Chuan |
| author_facet | Ng, Hui Suan Tan, Chin Ping Chen, Soo Kien Mokhtar, Mohd Noriznan Ariff, Arbakariya Ling, Tau Chuan |
| author_sort | Ng, Hui Suan |
| collection | UPM |
| description | In this works, the polymer–salt aqueous two phase system (ATPS) which is polyethylene–glycol (PEG) with sodium citrate was constructed to purify the enzyme CGTase from fermentation broth. Impacts of parameters such as phase composition, tie-line length (TLL), volume ratio (VR), crude sample loading, pH and the addition of sodium chloride (NaCl) on the partition behavior of cyclodextrin glycosyltransferase (CGTase) were investigated. The study exhibited that the optimum system for the purification of the enzyme CGTase was achieved on the 19.0% PEG and 11.5% citrate system with TLL of 38.89% (w/w), VR of 2.0, with additional 4% (w/w) NaCl and 20% crude load at pH 7.0. CGTase from Bacillus sp. was partially purified by the ATPS up to 16.3-fold with a yield of 70%. |
| first_indexed | 2024-03-06T07:53:32Z |
| format | Article |
| id | upm.eprints-22298 |
| institution | Universiti Putra Malaysia |
| language | English English |
| last_indexed | 2024-03-06T07:53:32Z |
| publishDate | 2011 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | upm.eprints-222982015-12-10T02:03:35Z http://psasir.upm.edu.my/id/eprint/22298/ Primary capture of cyclodextrin glycosyltransferase derived from Bacillus cereus by aqueous two phase system. Ng, Hui Suan Tan, Chin Ping Chen, Soo Kien Mokhtar, Mohd Noriznan Ariff, Arbakariya Ling, Tau Chuan In this works, the polymer–salt aqueous two phase system (ATPS) which is polyethylene–glycol (PEG) with sodium citrate was constructed to purify the enzyme CGTase from fermentation broth. Impacts of parameters such as phase composition, tie-line length (TLL), volume ratio (VR), crude sample loading, pH and the addition of sodium chloride (NaCl) on the partition behavior of cyclodextrin glycosyltransferase (CGTase) were investigated. The study exhibited that the optimum system for the purification of the enzyme CGTase was achieved on the 19.0% PEG and 11.5% citrate system with TLL of 38.89% (w/w), VR of 2.0, with additional 4% (w/w) NaCl and 20% crude load at pH 7.0. CGTase from Bacillus sp. was partially purified by the ATPS up to 16.3-fold with a yield of 70%. Elsevier 2011-10-10 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/22298/1/Primary%20capture%20of%20cyclodextrin%20glycosyltransferase%20derived%20from%20Bacillus%20cereus%20by%20aqueous%20two%20phase%20system.pdf Ng, Hui Suan and Tan, Chin Ping and Chen, Soo Kien and Mokhtar, Mohd Noriznan and Ariff, Arbakariya and Ling, Tau Chuan (2011) Primary capture of cyclodextrin glycosyltransferase derived from Bacillus cereus by aqueous two phase system. Separation and Purification Technology, 81 (3). pp. 318-324. ISSN 1383-5866 10.1016/j.seppur.2011.07.039 English |
| spellingShingle | Ng, Hui Suan Tan, Chin Ping Chen, Soo Kien Mokhtar, Mohd Noriznan Ariff, Arbakariya Ling, Tau Chuan Primary capture of cyclodextrin glycosyltransferase derived from Bacillus cereus by aqueous two phase system. |
| title | Primary capture of cyclodextrin glycosyltransferase derived from Bacillus cereus by aqueous two phase system. |
| title_full | Primary capture of cyclodextrin glycosyltransferase derived from Bacillus cereus by aqueous two phase system. |
| title_fullStr | Primary capture of cyclodextrin glycosyltransferase derived from Bacillus cereus by aqueous two phase system. |
| title_full_unstemmed | Primary capture of cyclodextrin glycosyltransferase derived from Bacillus cereus by aqueous two phase system. |
| title_short | Primary capture of cyclodextrin glycosyltransferase derived from Bacillus cereus by aqueous two phase system. |
| title_sort | primary capture of cyclodextrin glycosyltransferase derived from bacillus cereus by aqueous two phase system |
| url | http://psasir.upm.edu.my/id/eprint/22298/1/Primary%20capture%20of%20cyclodextrin%20glycosyltransferase%20derived%20from%20Bacillus%20cereus%20by%20aqueous%20two%20phase%20system.pdf |
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