Medium formulation and impeller design on the biosynthesis of high molecular weight hyaluronic acid by Streptococcus zooepidemicus ATCC 39920
The effects of medium formulation and impeller design (Rushton turbine and helical ribbon) on hyaluronic acid (HA) biosynthesis by Streptococcus zooepidemicus ATCC 39920 were investigated using a 2 L stirred-tank bioreactor. The effect of different glucose concentrations (20, 30, 50 and 60 g/L), nit...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Academic Journals
2011
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/22303/1/22303.pdf |
| _version_ | 1796970134744596480 |
|---|---|
| author | Lai, Zee Wei Abdul Rahim, Raha Ariff, Arbakariya Mohamad, Rosfarizan |
| author_facet | Lai, Zee Wei Abdul Rahim, Raha Ariff, Arbakariya Mohamad, Rosfarizan |
| author_sort | Lai, Zee Wei |
| collection | UPM |
| description | The effects of medium formulation and impeller design (Rushton turbine and helical ribbon) on hyaluronic acid (HA) biosynthesis by Streptococcus zooepidemicus ATCC 39920 were investigated using a 2 L stirred-tank bioreactor. The effect of different glucose concentrations (20, 30, 50 and 60 g/L), nitrogen sources ((NH4)2S2O8, (NH4)2PO4, yeast extract, and tryptone) and carbon/nitrogen ratios on the growth of the strain and on HA biosynthesis were initially investigated. Organic nitrogen sources (yeast extract and tryptone) were proven to be favourable in media for HA biosynthesis compared to inorganic nitrogen sources. About 2.442 g/L of HA with a high molecular weight (4.36 x 106Da) was synthesised at an optimal C/N of 2.5:1 (using a mixture of yeast extract and tryptone) in a 2 L stirred-tank bioreactor equipped with a Rushton turbine impeller. When using an optimal medium formulation at equal HA production levels, the helical ribbon impeller resulted in a higher molecular weight of HA (5.20 x 106 Da) compared to the Rushton turbine impeller. |
| first_indexed | 2024-03-06T07:53:33Z |
| format | Article |
| id | upm.eprints-22303 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T07:53:33Z |
| publishDate | 2011 |
| publisher | Academic Journals |
| record_format | dspace |
| spelling | upm.eprints-223032017-11-17T03:40:41Z http://psasir.upm.edu.my/id/eprint/22303/ Medium formulation and impeller design on the biosynthesis of high molecular weight hyaluronic acid by Streptococcus zooepidemicus ATCC 39920 Lai, Zee Wei Abdul Rahim, Raha Ariff, Arbakariya Mohamad, Rosfarizan The effects of medium formulation and impeller design (Rushton turbine and helical ribbon) on hyaluronic acid (HA) biosynthesis by Streptococcus zooepidemicus ATCC 39920 were investigated using a 2 L stirred-tank bioreactor. The effect of different glucose concentrations (20, 30, 50 and 60 g/L), nitrogen sources ((NH4)2S2O8, (NH4)2PO4, yeast extract, and tryptone) and carbon/nitrogen ratios on the growth of the strain and on HA biosynthesis were initially investigated. Organic nitrogen sources (yeast extract and tryptone) were proven to be favourable in media for HA biosynthesis compared to inorganic nitrogen sources. About 2.442 g/L of HA with a high molecular weight (4.36 x 106Da) was synthesised at an optimal C/N of 2.5:1 (using a mixture of yeast extract and tryptone) in a 2 L stirred-tank bioreactor equipped with a Rushton turbine impeller. When using an optimal medium formulation at equal HA production levels, the helical ribbon impeller resulted in a higher molecular weight of HA (5.20 x 106 Da) compared to the Rushton turbine impeller. Academic Journals 2011-08 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/22303/1/22303.pdf Lai, Zee Wei and Abdul Rahim, Raha and Ariff, Arbakariya and Mohamad, Rosfarizan (2011) Medium formulation and impeller design on the biosynthesis of high molecular weight hyaluronic acid by Streptococcus zooepidemicus ATCC 39920. African Journal of Microbiology Research, 5 (15). art. no. BDEA15D11917. pp. 2114-2123. ISSN 1996-0808 http://www.academicjournals.org/journal/AJMR/article-abstract/BDEA15D11917 |
| spellingShingle | Lai, Zee Wei Abdul Rahim, Raha Ariff, Arbakariya Mohamad, Rosfarizan Medium formulation and impeller design on the biosynthesis of high molecular weight hyaluronic acid by Streptococcus zooepidemicus ATCC 39920 |
| title | Medium formulation and impeller design on the biosynthesis of high molecular weight hyaluronic acid by Streptococcus zooepidemicus ATCC 39920 |
| title_full | Medium formulation and impeller design on the biosynthesis of high molecular weight hyaluronic acid by Streptococcus zooepidemicus ATCC 39920 |
| title_fullStr | Medium formulation and impeller design on the biosynthesis of high molecular weight hyaluronic acid by Streptococcus zooepidemicus ATCC 39920 |
| title_full_unstemmed | Medium formulation and impeller design on the biosynthesis of high molecular weight hyaluronic acid by Streptococcus zooepidemicus ATCC 39920 |
| title_short | Medium formulation and impeller design on the biosynthesis of high molecular weight hyaluronic acid by Streptococcus zooepidemicus ATCC 39920 |
| title_sort | medium formulation and impeller design on the biosynthesis of high molecular weight hyaluronic acid by streptococcus zooepidemicus atcc 39920 |
| url | http://psasir.upm.edu.my/id/eprint/22303/1/22303.pdf |
| work_keys_str_mv | AT laizeewei mediumformulationandimpellerdesignonthebiosynthesisofhighmolecularweighthyaluronicacidbystreptococcuszooepidemicusatcc39920 AT abdulrahimraha mediumformulationandimpellerdesignonthebiosynthesisofhighmolecularweighthyaluronicacidbystreptococcuszooepidemicusatcc39920 AT ariffarbakariya mediumformulationandimpellerdesignonthebiosynthesisofhighmolecularweighthyaluronicacidbystreptococcuszooepidemicusatcc39920 AT mohamadrosfarizan mediumformulationandimpellerdesignonthebiosynthesisofhighmolecularweighthyaluronicacidbystreptococcuszooepidemicusatcc39920 |