The effect of carbon sources on the expression level of thermostable L2 lipase in Pichia pastoris
Thermostable lipases are enzymes that are particularly pleasing for industrial purposes such as in the production of detergents, animal skin-based industry and food processing endeavours. Thermostable L2 lipase gene fished out from Bacillus sp. L2 was cloned into Pichia pastoris strain GS115 under c...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Academic Journals
2011
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| Online Access: | http://psasir.upm.edu.my/id/eprint/22323/1/22323.pdf |
| _version_ | 1825946850162638848 |
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| author | Shahidan, Noor Hidayah Raja Abdul Rahman, Raja Noor Zaliha Leow, Adam Thean Chor Mohamad, Rosfarizan Basri, Mahiran Salleh, Abu Bakar |
| author_facet | Shahidan, Noor Hidayah Raja Abdul Rahman, Raja Noor Zaliha Leow, Adam Thean Chor Mohamad, Rosfarizan Basri, Mahiran Salleh, Abu Bakar |
| author_sort | Shahidan, Noor Hidayah |
| collection | UPM |
| description | Thermostable lipases are enzymes that are particularly pleasing for industrial purposes such as in the production of detergents, animal skin-based industry and food processing endeavours. Thermostable L2 lipase gene fished out from Bacillus sp. L2 was cloned into Pichia pastoris strain GS115 under constitutive expression system of pGAPZαA. Study performed on various carbon sources revealed that glucose and glycerol support the growth of Pichia pastoris and expression of L2 lipase. In addition, the by-product of sugar refinery processes (molasses) was found to be a potential carbon source for the growth of P. pastoris and L2 lipase expression. Since the thermostable L2 lipase will be extracellularly secreted into the medium, the use of P. pastoris expression system is seen as an alternative to the conventional expression system of Escherichia coli that applies intracellular expression of the L2 lipase gene encoded. |
| first_indexed | 2024-03-06T07:53:37Z |
| format | Article |
| id | upm.eprints-22323 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T07:53:37Z |
| publishDate | 2011 |
| publisher | Academic Journals |
| record_format | dspace |
| spelling | upm.eprints-223232017-11-15T09:41:42Z http://psasir.upm.edu.my/id/eprint/22323/ The effect of carbon sources on the expression level of thermostable L2 lipase in Pichia pastoris Shahidan, Noor Hidayah Raja Abdul Rahman, Raja Noor Zaliha Leow, Adam Thean Chor Mohamad, Rosfarizan Basri, Mahiran Salleh, Abu Bakar Thermostable lipases are enzymes that are particularly pleasing for industrial purposes such as in the production of detergents, animal skin-based industry and food processing endeavours. Thermostable L2 lipase gene fished out from Bacillus sp. L2 was cloned into Pichia pastoris strain GS115 under constitutive expression system of pGAPZαA. Study performed on various carbon sources revealed that glucose and glycerol support the growth of Pichia pastoris and expression of L2 lipase. In addition, the by-product of sugar refinery processes (molasses) was found to be a potential carbon source for the growth of P. pastoris and L2 lipase expression. Since the thermostable L2 lipase will be extracellularly secreted into the medium, the use of P. pastoris expression system is seen as an alternative to the conventional expression system of Escherichia coli that applies intracellular expression of the L2 lipase gene encoded. Academic Journals 2011 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/22323/1/22323.pdf Shahidan, Noor Hidayah and Raja Abdul Rahman, Raja Noor Zaliha and Leow, Adam Thean Chor and Mohamad, Rosfarizan and Basri, Mahiran and Salleh, Abu Bakar (2011) The effect of carbon sources on the expression level of thermostable L2 lipase in Pichia pastoris. African Journal of Biotechnology, 10 (62). art. no. 305160A39043. pp. 13528-13535. ISSN 1684–5315 http://www.academicjournals.org/journal/AJB/article-abstract/305160A39043 10.5897/AJB11.1550 |
| spellingShingle | Shahidan, Noor Hidayah Raja Abdul Rahman, Raja Noor Zaliha Leow, Adam Thean Chor Mohamad, Rosfarizan Basri, Mahiran Salleh, Abu Bakar The effect of carbon sources on the expression level of thermostable L2 lipase in Pichia pastoris |
| title | The effect of carbon sources on the expression level of thermostable L2 lipase in Pichia pastoris |
| title_full | The effect of carbon sources on the expression level of thermostable L2 lipase in Pichia pastoris |
| title_fullStr | The effect of carbon sources on the expression level of thermostable L2 lipase in Pichia pastoris |
| title_full_unstemmed | The effect of carbon sources on the expression level of thermostable L2 lipase in Pichia pastoris |
| title_short | The effect of carbon sources on the expression level of thermostable L2 lipase in Pichia pastoris |
| title_sort | effect of carbon sources on the expression level of thermostable l2 lipase in pichia pastoris |
| url | http://psasir.upm.edu.my/id/eprint/22323/1/22323.pdf |
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