Bacteriocin release protein mediated secretory expression of recombinant chalcone synthase in Escherichia coli
Flavonoids are secondary metabolites synthesized by plants shown to exhibit health benefits such as anti-inflammatory, antioxidant, and anti-tumor effects. Thus, due to the importance of this compound, several enzymes involved in the flavonoid pathway have been cloned and characterized in Escherichi...
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| Format: | Article |
| Language: | English |
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Springer
2011
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| Online Access: | http://psasir.upm.edu.my/id/eprint/22324/1/Bacteriocin%20release%20protein%20mediated%20secretory%20expression%20of%20recombinant%20chalcone%20synthase%20in%20Escherichia%20coli.pdf |
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| author | Zakaria, Iffah Izzati Raja Abdul Rahman, Raja Noor Zaliha Salleh, Abu Bakar Basri, Mahiran |
| author_facet | Zakaria, Iffah Izzati Raja Abdul Rahman, Raja Noor Zaliha Salleh, Abu Bakar Basri, Mahiran |
| author_sort | Zakaria, Iffah Izzati |
| collection | UPM |
| description | Flavonoids are secondary metabolites synthesized by plants shown to exhibit health benefits such as anti-inflammatory, antioxidant, and anti-tumor effects. Thus, due to the importance of this compound, several enzymes involved in the flavonoid pathway have been cloned and characterized in Escherichia coli. However, the formation of inclusion bodies has become a major disadvantage of this approach. As an alternative, chalcone synthase from Physcomitrella patens was secreted into the medium using a bacteriocin release protein expression vector. Secretion of P. patens chalcone synthase into the culture media was achieved by co-expression with a psW1 plasmid encoding bacteriocin release protein in E. coli Tuner (DE3) plysS. The optimized conditions, which include the incubation of cells for 20 h with 40 ng/ml mitomycin C at OD600 induction time of 0.5 was found to be the best condition for chalcone synthase secretion. |
| first_indexed | 2024-03-06T07:53:37Z |
| format | Article |
| id | upm.eprints-22324 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T07:53:37Z |
| publishDate | 2011 |
| publisher | Springer |
| record_format | dspace |
| spelling | upm.eprints-223242016-09-26T07:09:14Z http://psasir.upm.edu.my/id/eprint/22324/ Bacteriocin release protein mediated secretory expression of recombinant chalcone synthase in Escherichia coli Zakaria, Iffah Izzati Raja Abdul Rahman, Raja Noor Zaliha Salleh, Abu Bakar Basri, Mahiran Flavonoids are secondary metabolites synthesized by plants shown to exhibit health benefits such as anti-inflammatory, antioxidant, and anti-tumor effects. Thus, due to the importance of this compound, several enzymes involved in the flavonoid pathway have been cloned and characterized in Escherichia coli. However, the formation of inclusion bodies has become a major disadvantage of this approach. As an alternative, chalcone synthase from Physcomitrella patens was secreted into the medium using a bacteriocin release protein expression vector. Secretion of P. patens chalcone synthase into the culture media was achieved by co-expression with a psW1 plasmid encoding bacteriocin release protein in E. coli Tuner (DE3) plysS. The optimized conditions, which include the incubation of cells for 20 h with 40 ng/ml mitomycin C at OD600 induction time of 0.5 was found to be the best condition for chalcone synthase secretion. Springer 2011 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/22324/1/Bacteriocin%20release%20protein%20mediated%20secretory%20expression%20of%20recombinant%20chalcone%20synthase%20in%20Escherichia%20coli.pdf Zakaria, Iffah Izzati and Raja Abdul Rahman, Raja Noor Zaliha and Salleh, Abu Bakar and Basri, Mahiran (2011) Bacteriocin release protein mediated secretory expression of recombinant chalcone synthase in Escherichia coli. Applied Biochemistry and Biotechnology, 165 (2). pp. 737-747. ISSN 0273-2289; ESSN: 1559-0291 http://link.springer.com/article/10.1007/s12010-011-9292-1?view=classic 10.1007/s12010-011-9292-1 |
| spellingShingle | Zakaria, Iffah Izzati Raja Abdul Rahman, Raja Noor Zaliha Salleh, Abu Bakar Basri, Mahiran Bacteriocin release protein mediated secretory expression of recombinant chalcone synthase in Escherichia coli |
| title | Bacteriocin release protein mediated secretory expression of recombinant chalcone synthase in Escherichia coli |
| title_full | Bacteriocin release protein mediated secretory expression of recombinant chalcone synthase in Escherichia coli |
| title_fullStr | Bacteriocin release protein mediated secretory expression of recombinant chalcone synthase in Escherichia coli |
| title_full_unstemmed | Bacteriocin release protein mediated secretory expression of recombinant chalcone synthase in Escherichia coli |
| title_short | Bacteriocin release protein mediated secretory expression of recombinant chalcone synthase in Escherichia coli |
| title_sort | bacteriocin release protein mediated secretory expression of recombinant chalcone synthase in escherichia coli |
| url | http://psasir.upm.edu.my/id/eprint/22324/1/Bacteriocin%20release%20protein%20mediated%20secretory%20expression%20of%20recombinant%20chalcone%20synthase%20in%20Escherichia%20coli.pdf |
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