Kinetics of cellulase production by Aspergillus terreus at various levels of dissolved oxygen tension in a stirred tank bioreactor
In this study the effect of different levels of dissolved oxygen tension (d.o.t) on the production of three main components of extracellular cellulases (FPase, CMCase and β-glucosidase) at a fixed agitation speed by Aspergillus terreus was investigated. Growth of A. terreus and cellulase production...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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North Carolina State University
2011
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| Online Access: | http://psasir.upm.edu.my/id/eprint/22326/1/Kinetics%20of%20cellulase%20production%20by%20Aspergillus%20terreus%20at%20various%20levels%20of%20dissolved%20oxygen%20tension%20in%20a%20stirred%20tank%20bioreactor.pdf |
| _version_ | 1796970139378253824 |
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| author | Shahriarinour, Mahdi Abdul Wahab, Mohd Noor Ariff, Arbakariya Mohamad, Rosfarizan Mustafa, Shuhaimi |
| author_facet | Shahriarinour, Mahdi Abdul Wahab, Mohd Noor Ariff, Arbakariya Mohamad, Rosfarizan Mustafa, Shuhaimi |
| author_sort | Shahriarinour, Mahdi |
| collection | UPM |
| description | In this study the effect of different levels of dissolved oxygen tension (d.o.t) on the production of three main components of extracellular cellulases (FPase, CMCase and β-glucosidase) at a fixed agitation speed by Aspergillus terreus was investigated. Growth of A. terreus and cellulase production were modeled based on logistic and Luedeking-Piret equations. The results from the model fit well with the experimental data, confirming that the models were appropriate for describing and representing growth and cellulase production at various d.o.t levels. The models showed that the production of FPase and CMCase were growth-associated processes. Cell growth and cellulase production were approximately two-fold higher in a stirred tank bioreactor compared with a shake-flask culture. At a d.o.t of 55% air saturation, cell growth and cellulase production were higher than at low d.o.t (40% air saturation) or high d.o.t (80% air saturation). The highest activities of FPase (2.33 U ml-1), CMCase (51.10 U ml-1), and β-glucosidase (16.18 U ml-1) were obtained at a d.o.t of 55% air saturation, yielding overall productivities of 19.40, 425.00, and 67.40 U l.h-1, respectively. |
| first_indexed | 2024-03-06T07:53:37Z |
| format | Article |
| id | upm.eprints-22326 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T07:53:37Z |
| publishDate | 2011 |
| publisher | North Carolina State University |
| record_format | dspace |
| spelling | upm.eprints-223262016-03-15T00:54:49Z http://psasir.upm.edu.my/id/eprint/22326/ Kinetics of cellulase production by Aspergillus terreus at various levels of dissolved oxygen tension in a stirred tank bioreactor Shahriarinour, Mahdi Abdul Wahab, Mohd Noor Ariff, Arbakariya Mohamad, Rosfarizan Mustafa, Shuhaimi In this study the effect of different levels of dissolved oxygen tension (d.o.t) on the production of three main components of extracellular cellulases (FPase, CMCase and β-glucosidase) at a fixed agitation speed by Aspergillus terreus was investigated. Growth of A. terreus and cellulase production were modeled based on logistic and Luedeking-Piret equations. The results from the model fit well with the experimental data, confirming that the models were appropriate for describing and representing growth and cellulase production at various d.o.t levels. The models showed that the production of FPase and CMCase were growth-associated processes. Cell growth and cellulase production were approximately two-fold higher in a stirred tank bioreactor compared with a shake-flask culture. At a d.o.t of 55% air saturation, cell growth and cellulase production were higher than at low d.o.t (40% air saturation) or high d.o.t (80% air saturation). The highest activities of FPase (2.33 U ml-1), CMCase (51.10 U ml-1), and β-glucosidase (16.18 U ml-1) were obtained at a d.o.t of 55% air saturation, yielding overall productivities of 19.40, 425.00, and 67.40 U l.h-1, respectively. North Carolina State University 2011 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/22326/1/Kinetics%20of%20cellulase%20production%20by%20Aspergillus%20terreus%20at%20various%20levels%20of%20dissolved%20oxygen%20tension%20in%20a%20stirred%20tank%20bioreactor.pdf Shahriarinour, Mahdi and Abdul Wahab, Mohd Noor and Ariff, Arbakariya and Mohamad, Rosfarizan and Mustafa, Shuhaimi (2011) Kinetics of cellulase production by Aspergillus terreus at various levels of dissolved oxygen tension in a stirred tank bioreactor. BioResources, 6 (4). pp. 4909-4921. ISSN 1930-2126 http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_06_4_4909_Shahriarinour_WAMM_Kinetics_Cellulase_Prod_Aspergillus |
| spellingShingle | Shahriarinour, Mahdi Abdul Wahab, Mohd Noor Ariff, Arbakariya Mohamad, Rosfarizan Mustafa, Shuhaimi Kinetics of cellulase production by Aspergillus terreus at various levels of dissolved oxygen tension in a stirred tank bioreactor |
| title | Kinetics of cellulase production by Aspergillus terreus at various levels of dissolved oxygen tension in a stirred tank bioreactor |
| title_full | Kinetics of cellulase production by Aspergillus terreus at various levels of dissolved oxygen tension in a stirred tank bioreactor |
| title_fullStr | Kinetics of cellulase production by Aspergillus terreus at various levels of dissolved oxygen tension in a stirred tank bioreactor |
| title_full_unstemmed | Kinetics of cellulase production by Aspergillus terreus at various levels of dissolved oxygen tension in a stirred tank bioreactor |
| title_short | Kinetics of cellulase production by Aspergillus terreus at various levels of dissolved oxygen tension in a stirred tank bioreactor |
| title_sort | kinetics of cellulase production by aspergillus terreus at various levels of dissolved oxygen tension in a stirred tank bioreactor |
| url | http://psasir.upm.edu.my/id/eprint/22326/1/Kinetics%20of%20cellulase%20production%20by%20Aspergillus%20terreus%20at%20various%20levels%20of%20dissolved%20oxygen%20tension%20in%20a%20stirred%20tank%20bioreactor.pdf |
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