Transcripts of Gracilaria changii that improve copper tolerance of Escherichia coli
In this study, we used bacterial functional screening to isolate transcripts from a red seaweed, Gracilaria changii Abbott, Zhang et Xia (Xia et Abbott) that improved copper tolerance of Escherichia coli. We have identified several seaweed proteins that may be involved in copper efflux, detoxificati...
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| Format: | Article |
| Language: | English |
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Malaysian Society for Molecular Biology and Biotechnology
2010
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| Online Access: | http://psasir.upm.edu.my/id/eprint/22345/1/Transcripts%20of%20Gracilaria%20changii%20that%20improve%20copper%20tolerance%20of%20Escherichia%20coli.pdf |
| _version_ | 1825946854494306304 |
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| author | Ho, Chai Ling Teo, Swee Sen Abdul Rahim, Raha Phang, Siew Moi |
| author_facet | Ho, Chai Ling Teo, Swee Sen Abdul Rahim, Raha Phang, Siew Moi |
| author_sort | Ho, Chai Ling |
| collection | UPM |
| description | In this study, we used bacterial functional screening to isolate transcripts from a red seaweed, Gracilaria changii Abbott, Zhang et Xia (Xia et Abbott) that improved copper tolerance of Escherichia coli. We have identified several seaweed proteins that may be involved in copper efflux, detoxification and ROS scavenging, such as ATPase, outward-rectifying potassium channel (KCO1), vanadium chloroperoxidase and a high affinity phosphate transporter. All transcripts were shown to be able to enhance the copper tolerance of E. coli up to 4 mM CuCl2. These transcripts may share similar functions in the copper homeostasis of both E. coli and G. changii. In addition, we discovered a few transcripts with uncharacterized function(s) in copper tolerance in both organisms. |
| first_indexed | 2024-03-06T07:53:41Z |
| format | Article |
| id | upm.eprints-22345 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T07:53:41Z |
| publishDate | 2010 |
| publisher | Malaysian Society for Molecular Biology and Biotechnology |
| record_format | dspace |
| spelling | upm.eprints-223452015-09-15T03:05:53Z http://psasir.upm.edu.my/id/eprint/22345/ Transcripts of Gracilaria changii that improve copper tolerance of Escherichia coli Ho, Chai Ling Teo, Swee Sen Abdul Rahim, Raha Phang, Siew Moi In this study, we used bacterial functional screening to isolate transcripts from a red seaweed, Gracilaria changii Abbott, Zhang et Xia (Xia et Abbott) that improved copper tolerance of Escherichia coli. We have identified several seaweed proteins that may be involved in copper efflux, detoxification and ROS scavenging, such as ATPase, outward-rectifying potassium channel (KCO1), vanadium chloroperoxidase and a high affinity phosphate transporter. All transcripts were shown to be able to enhance the copper tolerance of E. coli up to 4 mM CuCl2. These transcripts may share similar functions in the copper homeostasis of both E. coli and G. changii. In addition, we discovered a few transcripts with uncharacterized function(s) in copper tolerance in both organisms. Malaysian Society for Molecular Biology and Biotechnology 2010 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/22345/1/Transcripts%20of%20Gracilaria%20changii%20that%20improve%20copper%20tolerance%20of%20Escherichia%20coli.pdf Ho, Chai Ling and Teo, Swee Sen and Abdul Rahim, Raha and Phang, Siew Moi (2010) Transcripts of Gracilaria changii that improve copper tolerance of Escherichia coli. Asia Pacific Journal of Molecular Biology and Biotechnology, 18 (3). pp. 315-319. ISSN 0128-7451 |
| spellingShingle | Ho, Chai Ling Teo, Swee Sen Abdul Rahim, Raha Phang, Siew Moi Transcripts of Gracilaria changii that improve copper tolerance of Escherichia coli |
| title | Transcripts of Gracilaria changii that improve copper tolerance of Escherichia coli |
| title_full | Transcripts of Gracilaria changii that improve copper tolerance of Escherichia coli |
| title_fullStr | Transcripts of Gracilaria changii that improve copper tolerance of Escherichia coli |
| title_full_unstemmed | Transcripts of Gracilaria changii that improve copper tolerance of Escherichia coli |
| title_short | Transcripts of Gracilaria changii that improve copper tolerance of Escherichia coli |
| title_sort | transcripts of gracilaria changii that improve copper tolerance of escherichia coli |
| url | http://psasir.upm.edu.my/id/eprint/22345/1/Transcripts%20of%20Gracilaria%20changii%20that%20improve%20copper%20tolerance%20of%20Escherichia%20coli.pdf |
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