Identification of microRNA precursors in Bruguiera spp.
MicroRNAs (miRNA) are approximately 22 nt single stranded functional RNAs derived from long stem-loop precursors transcribed by RNA polymerase II. They regulate gene expression through post-transcriptional gene silencing and are important for the regulation of growth, development and stress response...
| Autores principales: | , , , |
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| Formato: | Artículo |
| Lenguaje: | English |
| Publicado: |
Walter de Gruyter GmbH
2011
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| Acceso en línea: | http://psasir.upm.edu.my/id/eprint/22346/1/Identification%20of%20microRNA%20precursors%20in%20Bruguiera%20spp.pdf |
| _version_ | 1825946854706118656 |
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| author | Lee, Wan Sin Leong, Pei Li Ho, Chai Ling Harikrishna, Jennifer Ann |
| author_facet | Lee, Wan Sin Leong, Pei Li Ho, Chai Ling Harikrishna, Jennifer Ann |
| author_sort | Lee, Wan Sin |
| collection | UPM |
| description | MicroRNAs (miRNA) are approximately 22 nt single stranded functional RNAs derived from long stem-loop precursors transcribed by RNA polymerase II. They regulate gene expression through post-transcriptional gene silencing and are important for the regulation of growth, development and stress responses in plants. Mature nucleotide sequences of many miRNA families are highly conserved across the plant kingdom and can be used to identify and annotate homologs and potential miRNA targets. In this study, mature miRNA sequences retrieved from the miRNA registry (miRBase) were used to identify precursor sequences of miRNA orthologs and their potential targets among Expressed Sequence Tags (ESTs) of the mangrove species Bruguiera cylindrica (L.) Blume, B. gymnorhiza (L.) Lam. and B. sexangula (Lour.) Poir. Candidate miRNA precursors, which potentially belong to the miR156/7, miR396 and miR529 families, had high sequence identity between Bruguiera cylindrica and Bruguiera gymnorhiza, and expression of RNA was confirmed in both species. A number of candidate targets for miR396 and miR529 were also identified among EST from B. gymnorhiza. |
| first_indexed | 2024-03-06T07:53:41Z |
| format | Article |
| id | upm.eprints-22346 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T07:53:41Z |
| publishDate | 2011 |
| publisher | Walter de Gruyter GmbH |
| record_format | dspace |
| spelling | upm.eprints-223462015-10-05T01:45:15Z http://psasir.upm.edu.my/id/eprint/22346/ Identification of microRNA precursors in Bruguiera spp. Lee, Wan Sin Leong, Pei Li Ho, Chai Ling Harikrishna, Jennifer Ann MicroRNAs (miRNA) are approximately 22 nt single stranded functional RNAs derived from long stem-loop precursors transcribed by RNA polymerase II. They regulate gene expression through post-transcriptional gene silencing and are important for the regulation of growth, development and stress responses in plants. Mature nucleotide sequences of many miRNA families are highly conserved across the plant kingdom and can be used to identify and annotate homologs and potential miRNA targets. In this study, mature miRNA sequences retrieved from the miRNA registry (miRBase) were used to identify precursor sequences of miRNA orthologs and their potential targets among Expressed Sequence Tags (ESTs) of the mangrove species Bruguiera cylindrica (L.) Blume, B. gymnorhiza (L.) Lam. and B. sexangula (Lour.) Poir. Candidate miRNA precursors, which potentially belong to the miR156/7, miR396 and miR529 families, had high sequence identity between Bruguiera cylindrica and Bruguiera gymnorhiza, and expression of RNA was confirmed in both species. A number of candidate targets for miR396 and miR529 were also identified among EST from B. gymnorhiza. Walter de Gruyter GmbH 2011 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/22346/1/Identification%20of%20microRNA%20precursors%20in%20Bruguiera%20spp.pdf Lee, Wan Sin and Leong, Pei Li and Ho, Chai Ling and Harikrishna, Jennifer Ann (2011) Identification of microRNA precursors in Bruguiera spp. Botanica Marina, 54 (3). pp. 313-324. ISSN 0006-8055; ESSN: 1437-4323 http://www.degruyter.com/view/j/botm.2011.54.issue-3/bot.2011.031/bot.2011.031.xml 10.1515/bot.2011.031 |
| spellingShingle | Lee, Wan Sin Leong, Pei Li Ho, Chai Ling Harikrishna, Jennifer Ann Identification of microRNA precursors in Bruguiera spp. |
| title | Identification of microRNA precursors in Bruguiera spp. |
| title_full | Identification of microRNA precursors in Bruguiera spp. |
| title_fullStr | Identification of microRNA precursors in Bruguiera spp. |
| title_full_unstemmed | Identification of microRNA precursors in Bruguiera spp. |
| title_short | Identification of microRNA precursors in Bruguiera spp. |
| title_sort | identification of microrna precursors in bruguiera spp |
| url | http://psasir.upm.edu.my/id/eprint/22346/1/Identification%20of%20microRNA%20precursors%20in%20Bruguiera%20spp.pdf |
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