Evaluation of anti-inflammatory, antioxidant and anti-nociceptive activities of six Malaysian medicinal plants
The anti-inflammatory, antioxidant and anti-nociceptive properties of six Malaysian medicinal plants, including Carica papaya, Musa acuminata, Oenanthe javanica, Piper sarmentosum, Psophocarpus tetragonolobus and Sauropus androgynus were investigated. The anti-inflammatory effects of the methanolic...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Academic Journals
2011
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| Online Access: | http://psasir.upm.edu.my/id/eprint/22372/1/Evaluation%20of%20anti.pdf |
| _version_ | 1825946859549491200 |
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| author | Lee, Kun Hong Mohd Padzil, Azyyati Ahmad, Syahida Abdullah, Norhani Saad, Wan Zuhainis Mahmood, Maziah Sulaiman, Mohd Roslan Ahmad Israf, Daud Shaari, Khozirah Lajis, Md. Nordin |
| author_facet | Lee, Kun Hong Mohd Padzil, Azyyati Ahmad, Syahida Abdullah, Norhani Saad, Wan Zuhainis Mahmood, Maziah Sulaiman, Mohd Roslan Ahmad Israf, Daud Shaari, Khozirah Lajis, Md. Nordin |
| author_sort | Lee, Kun Hong |
| collection | UPM |
| description | The anti-inflammatory, antioxidant and anti-nociceptive properties of six Malaysian medicinal plants, including Carica papaya, Musa acuminata, Oenanthe javanica, Piper sarmentosum, Psophocarpus tetragonolobus and Sauropus androgynus were investigated. The anti-inflammatory effects of the methanolic plant extracts were evaluated by using Griess assay on nitric oxide inhibitory activity upon IFN-g/LPS stimulated RAW 264.7 cells. The antioxidant property and total phenolic content of the plant extracts were determined by using DPPH radical scavenging assay and Folin-Ciocalteu’s assay, respectively. Anti-nociceptive activity of the plant extracts were evaluated by measuring the number of writhing response of mice upon acetic acid induction. All plant species showed significant nitric oxide (NO) inhibitory activity (IC50 <61 µg/ml) without causing cytotoxicity to RAW 264.7 cells. Besides, all six plants exhibited different degree of antioxidant activities (IC50 value, 86.74 ± 2.92 to 192.92 ± 2.60 µg/ml). The antioxidant activity might be due to the present of phenolic compounds (34.20 to 50.01 mg GA/g DW samples). Moreover, all plant species suppress the writhing response of mice at different degree of inhibition (10.65 to 43.12% inhibition) at concentration tested of 200 mg/kg. Thus, this study validates the traditional medicinal uses of the evaluated plant species in management of inflammation and free radical related disorders. |
| first_indexed | 2024-03-06T07:53:45Z |
| format | Article |
| id | upm.eprints-22372 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T07:53:45Z |
| publishDate | 2011 |
| publisher | Academic Journals |
| record_format | dspace |
| spelling | upm.eprints-223722015-10-02T03:48:36Z http://psasir.upm.edu.my/id/eprint/22372/ Evaluation of anti-inflammatory, antioxidant and anti-nociceptive activities of six Malaysian medicinal plants Lee, Kun Hong Mohd Padzil, Azyyati Ahmad, Syahida Abdullah, Norhani Saad, Wan Zuhainis Mahmood, Maziah Sulaiman, Mohd Roslan Ahmad Israf, Daud Shaari, Khozirah Lajis, Md. Nordin The anti-inflammatory, antioxidant and anti-nociceptive properties of six Malaysian medicinal plants, including Carica papaya, Musa acuminata, Oenanthe javanica, Piper sarmentosum, Psophocarpus tetragonolobus and Sauropus androgynus were investigated. The anti-inflammatory effects of the methanolic plant extracts were evaluated by using Griess assay on nitric oxide inhibitory activity upon IFN-g/LPS stimulated RAW 264.7 cells. The antioxidant property and total phenolic content of the plant extracts were determined by using DPPH radical scavenging assay and Folin-Ciocalteu’s assay, respectively. Anti-nociceptive activity of the plant extracts were evaluated by measuring the number of writhing response of mice upon acetic acid induction. All plant species showed significant nitric oxide (NO) inhibitory activity (IC50 <61 µg/ml) without causing cytotoxicity to RAW 264.7 cells. Besides, all six plants exhibited different degree of antioxidant activities (IC50 value, 86.74 ± 2.92 to 192.92 ± 2.60 µg/ml). The antioxidant activity might be due to the present of phenolic compounds (34.20 to 50.01 mg GA/g DW samples). Moreover, all plant species suppress the writhing response of mice at different degree of inhibition (10.65 to 43.12% inhibition) at concentration tested of 200 mg/kg. Thus, this study validates the traditional medicinal uses of the evaluated plant species in management of inflammation and free radical related disorders. Academic Journals 2011-10 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/22372/1/Evaluation%20of%20anti.pdf Lee, Kun Hong and Mohd Padzil, Azyyati and Ahmad, Syahida and Abdullah, Norhani and Saad, Wan Zuhainis and Mahmood, Maziah and Sulaiman, Mohd Roslan and Ahmad Israf, Daud and Shaari, Khozirah and Lajis, Md. Nordin (2011) Evaluation of anti-inflammatory, antioxidant and anti-nociceptive activities of six Malaysian medicinal plants. Journal of Medicinal Plants Research, 5 (23). pp. 5555-5563. ISSN 1996-0875 http://www.academicjournals.org/journal/JMPR/article-abstract/B77CA3017187 |
| spellingShingle | Lee, Kun Hong Mohd Padzil, Azyyati Ahmad, Syahida Abdullah, Norhani Saad, Wan Zuhainis Mahmood, Maziah Sulaiman, Mohd Roslan Ahmad Israf, Daud Shaari, Khozirah Lajis, Md. Nordin Evaluation of anti-inflammatory, antioxidant and anti-nociceptive activities of six Malaysian medicinal plants |
| title | Evaluation of anti-inflammatory, antioxidant and anti-nociceptive activities of six Malaysian medicinal plants |
| title_full | Evaluation of anti-inflammatory, antioxidant and anti-nociceptive activities of six Malaysian medicinal plants |
| title_fullStr | Evaluation of anti-inflammatory, antioxidant and anti-nociceptive activities of six Malaysian medicinal plants |
| title_full_unstemmed | Evaluation of anti-inflammatory, antioxidant and anti-nociceptive activities of six Malaysian medicinal plants |
| title_short | Evaluation of anti-inflammatory, antioxidant and anti-nociceptive activities of six Malaysian medicinal plants |
| title_sort | evaluation of anti inflammatory antioxidant and anti nociceptive activities of six malaysian medicinal plants |
| url | http://psasir.upm.edu.my/id/eprint/22372/1/Evaluation%20of%20anti.pdf |
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