Screening, isolation and selection of cellulolytic fungi from oil palm empty fruit bunch fibre

Screening and isolation of cellulolytic fungi was done using compost of oil palm empty fruit bunch conducted at a local factory located in Sri Ulu Langat, Dengkil, Selangor, Malaysia. This research was aimed to isolate of cellulolytic fungi with over production of cellulase components. Ten isolated...

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Main Authors: Shahriarinour, Mahdi, Abdul Wahab, Mohd Noor, Ariff, Arbakariya, Mohamad, Rosfarizan
Format: Article
Language:English
Published: Asian Network for Scientific Information 2011
Online Access:http://psasir.upm.edu.my/id/eprint/22412/1/Screening%2C%20isolation%20and%20selection%20of%20cellulolytic%20fungi%20from%20oil%20palm%20empty%20fruit%20bunch%20fibre.pdf
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author Shahriarinour, Mahdi
Abdul Wahab, Mohd Noor
Ariff, Arbakariya
Mohamad, Rosfarizan
author_facet Shahriarinour, Mahdi
Abdul Wahab, Mohd Noor
Ariff, Arbakariya
Mohamad, Rosfarizan
author_sort Shahriarinour, Mahdi
collection UPM
description Screening and isolation of cellulolytic fungi was done using compost of oil palm empty fruit bunch conducted at a local factory located in Sri Ulu Langat, Dengkil, Selangor, Malaysia. This research was aimed to isolate of cellulolytic fungi with over production of cellulase components. Ten isolated fungi had shown the ability to degrade cellulose base on decolorization of CMC selective agar using Grams iodine as color indicator and cellulase production in shake flask fermentation. Aspergillus (R4) was selected as over producer of cellulase enzyme among ten isolated fungi. The Aspergillus (R4) with highest clearing zone on CMC agar (50 mm) and cellulolytic activity (CMCase 3.05, FPase 0.61 and β-glucosidase 1.75 U mL-1) was identified as Aspergillus terrus. We identified Aspergillus terrues as highly cellulolytic in producing cellulase activity. Identification of over producers of cellulase enzymes can help industries exploits of this enzyme.
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spelling upm.eprints-224122016-03-15T00:55:47Z http://psasir.upm.edu.my/id/eprint/22412/ Screening, isolation and selection of cellulolytic fungi from oil palm empty fruit bunch fibre Shahriarinour, Mahdi Abdul Wahab, Mohd Noor Ariff, Arbakariya Mohamad, Rosfarizan Screening and isolation of cellulolytic fungi was done using compost of oil palm empty fruit bunch conducted at a local factory located in Sri Ulu Langat, Dengkil, Selangor, Malaysia. This research was aimed to isolate of cellulolytic fungi with over production of cellulase components. Ten isolated fungi had shown the ability to degrade cellulose base on decolorization of CMC selective agar using Grams iodine as color indicator and cellulase production in shake flask fermentation. Aspergillus (R4) was selected as over producer of cellulase enzyme among ten isolated fungi. The Aspergillus (R4) with highest clearing zone on CMC agar (50 mm) and cellulolytic activity (CMCase 3.05, FPase 0.61 and β-glucosidase 1.75 U mL-1) was identified as Aspergillus terrus. We identified Aspergillus terrues as highly cellulolytic in producing cellulase activity. Identification of over producers of cellulase enzymes can help industries exploits of this enzyme. Asian Network for Scientific Information 2011 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/22412/1/Screening%2C%20isolation%20and%20selection%20of%20cellulolytic%20fungi%20from%20oil%20palm%20empty%20fruit%20bunch%20fibre.pdf Shahriarinour, Mahdi and Abdul Wahab, Mohd Noor and Ariff, Arbakariya and Mohamad, Rosfarizan (2011) Screening, isolation and selection of cellulolytic fungi from oil palm empty fruit bunch fibre. Biotechnology, 10 (1). pp. 108-113. ISSN 1682-296X; ESSN: 1682-2978 http://scialert.net/abstract/?doi=biotech.2011.108.113 10.3923/biotech.2011.108.113
spellingShingle Shahriarinour, Mahdi
Abdul Wahab, Mohd Noor
Ariff, Arbakariya
Mohamad, Rosfarizan
Screening, isolation and selection of cellulolytic fungi from oil palm empty fruit bunch fibre
title Screening, isolation and selection of cellulolytic fungi from oil palm empty fruit bunch fibre
title_full Screening, isolation and selection of cellulolytic fungi from oil palm empty fruit bunch fibre
title_fullStr Screening, isolation and selection of cellulolytic fungi from oil palm empty fruit bunch fibre
title_full_unstemmed Screening, isolation and selection of cellulolytic fungi from oil palm empty fruit bunch fibre
title_short Screening, isolation and selection of cellulolytic fungi from oil palm empty fruit bunch fibre
title_sort screening isolation and selection of cellulolytic fungi from oil palm empty fruit bunch fibre
url http://psasir.upm.edu.my/id/eprint/22412/1/Screening%2C%20isolation%20and%20selection%20of%20cellulolytic%20fungi%20from%20oil%20palm%20empty%20fruit%20bunch%20fibre.pdf
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