Effect of medium composition and cultural condition on cellulase production by Aspergillus terreus
The effect of medium composition and environmental condition on the production of cellulase by Aspergillus terreus was investigated using shake flask culture with oil palm empty fruit bunch (OPEFB) as substrate. The highest activity of FPase (0.76 U ml-1), CMCase (8.64 U ml-1) and β-glucosidase (6.8...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Academic Journals
2011
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| Online Access: | http://psasir.upm.edu.my/id/eprint/22413/1/Effect%20of%20medium%20composition%20and%20cultural%20condition%20on.pdf |
| _version_ | 1825946866370478080 |
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| author | Shahriarinour, Mahdi Abdul Wahab, Mohd Noor Mohamad, Rosfarizan Mustafa, Shuhaimi Ariff, Arbakariya |
| author_facet | Shahriarinour, Mahdi Abdul Wahab, Mohd Noor Mohamad, Rosfarizan Mustafa, Shuhaimi Ariff, Arbakariya |
| author_sort | Shahriarinour, Mahdi |
| collection | UPM |
| description | The effect of medium composition and environmental condition on the production of cellulase by Aspergillus terreus was investigated using shake flask culture with oil palm empty fruit bunch (OPEFB) as substrate. The highest activity of FPase (0.76 U ml-1), CMCase (8.64 U ml-1) and β-glucosidase (6.81 U ml-1) was obtained in medium containing 6 g L-1 yeast extract and 10 g L-1 delignified OPEFB fiber. In fermentation with the addition of Tween 80 (2 ml L-1) as surfactant, the production of cellulase was increased by two-fold as compared to fermentation without surfactant. Cellulase production by A. terreus was also enhanced with the addition of calcium chloride (3 mM) and magnesium sulfate (5 mM) in the medium. Optimum pH and temperature for cellulase production by A. terreus was 5.5 and 28°C, respectively. Cellulase production in agitated shake flask fermentation at 200 rpm was four times higher as compared to fermentation in static flask. |
| first_indexed | 2024-03-06T07:53:52Z |
| format | Article |
| id | upm.eprints-22413 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T07:53:52Z |
| publishDate | 2011 |
| publisher | Academic Journals |
| record_format | dspace |
| spelling | upm.eprints-224132017-11-14T02:03:17Z http://psasir.upm.edu.my/id/eprint/22413/ Effect of medium composition and cultural condition on cellulase production by Aspergillus terreus Shahriarinour, Mahdi Abdul Wahab, Mohd Noor Mohamad, Rosfarizan Mustafa, Shuhaimi Ariff, Arbakariya The effect of medium composition and environmental condition on the production of cellulase by Aspergillus terreus was investigated using shake flask culture with oil palm empty fruit bunch (OPEFB) as substrate. The highest activity of FPase (0.76 U ml-1), CMCase (8.64 U ml-1) and β-glucosidase (6.81 U ml-1) was obtained in medium containing 6 g L-1 yeast extract and 10 g L-1 delignified OPEFB fiber. In fermentation with the addition of Tween 80 (2 ml L-1) as surfactant, the production of cellulase was increased by two-fold as compared to fermentation without surfactant. Cellulase production by A. terreus was also enhanced with the addition of calcium chloride (3 mM) and magnesium sulfate (5 mM) in the medium. Optimum pH and temperature for cellulase production by A. terreus was 5.5 and 28°C, respectively. Cellulase production in agitated shake flask fermentation at 200 rpm was four times higher as compared to fermentation in static flask. Academic Journals 2011-07-25 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/22413/1/Effect%20of%20medium%20composition%20and%20cultural%20condition%20on.pdf Shahriarinour, Mahdi and Abdul Wahab, Mohd Noor and Mohamad, Rosfarizan and Mustafa, Shuhaimi and Ariff, Arbakariya (2011) Effect of medium composition and cultural condition on cellulase production by Aspergillus terreus. African Journal of Biotechnology, 10 (38). art. no. 2C68E2D33210. pp. 7459-7467. ISSN 1684–5315 http://www.academicjournals.org/journal/AJB/article-abstract/2C68E2D33210 |
| spellingShingle | Shahriarinour, Mahdi Abdul Wahab, Mohd Noor Mohamad, Rosfarizan Mustafa, Shuhaimi Ariff, Arbakariya Effect of medium composition and cultural condition on cellulase production by Aspergillus terreus |
| title | Effect of medium composition and cultural condition on cellulase production by Aspergillus terreus |
| title_full | Effect of medium composition and cultural condition on cellulase production by Aspergillus terreus |
| title_fullStr | Effect of medium composition and cultural condition on cellulase production by Aspergillus terreus |
| title_full_unstemmed | Effect of medium composition and cultural condition on cellulase production by Aspergillus terreus |
| title_short | Effect of medium composition and cultural condition on cellulase production by Aspergillus terreus |
| title_sort | effect of medium composition and cultural condition on cellulase production by aspergillus terreus |
| url | http://psasir.upm.edu.my/id/eprint/22413/1/Effect%20of%20medium%20composition%20and%20cultural%20condition%20on.pdf |
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