Isolation, cloning and characterisation of new fragrance-related floral transcripts of Vanda Mimi Palmer
A subtracted cDNA library of open flower was constructed using the suppression subtractive hybridization (SSH) technique, to identify fragrance-related transcripts of Vanda Mimi Palmer. In total, 107 transcripts up-regulated during blooming were identified and sequenced. Only 33 clones (4 singletons...
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| Format: | Article |
| Language: | English |
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Elsevier
2011
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| Online Access: | http://psasir.upm.edu.my/id/eprint/22419/1/Isolation%2C%20cloning%20adn%20characteristion%20of%20new%20fragrance.pdf |
| _version_ | 1825946867535446016 |
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| author | Chan, Wai Sun Abdullah, Janna Ong Namasivayam, Parameswari |
| author_facet | Chan, Wai Sun Abdullah, Janna Ong Namasivayam, Parameswari |
| author_sort | Chan, Wai Sun |
| collection | UPM |
| description | A subtracted cDNA library of open flower was constructed using the suppression subtractive hybridization (SSH) technique, to identify fragrance-related transcripts of Vanda Mimi Palmer. In total, 107 transcripts up-regulated during blooming were identified and sequenced. Only 33 clones (4 singletons and 29 contigs) showed similarities to known sequences in the public database. Of these, thirty-two clones were transcripts encoding fragrance-related enzymes including sesquiterpene synthase, (±)-germacrene D synthase, tyrosine decarboxylase and putative acyltransferase. Two fragrance-related transcripts, VMPAAT encoding a putative alcohol acyltransferase and VMPSTS encoding a sesquiterpene synthase, were subjected to full-length cDNA isolation and characterization. The full length cDNA of VMPAAT has a 1343bp open reading frame (ORF) of 448 amino acid residues whereas VMPSTS is predicted to encode a polypeptide of 561 amino acid residues with 1682bp ORF. VMPAAT and VMPSTS show high homologies with plant alcohol acyltransferase and terpene synthase, respectively. Real time RT-PCR indicated that both transcripts were expressed preferentially in floral tissues, with high levels in blooming and full bloom flowers. VMPAAT and VMPSTS transcripts were expressed in a rhythmic pattern. The results presented in this study will be potentially useful in providing additional insights into the fragrance-related pathways of Orchidaceae members, which until today is still limited.
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| first_indexed | 2024-03-06T07:53:53Z |
| format | Article |
| id | upm.eprints-22419 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T07:53:53Z |
| publishDate | 2011 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | upm.eprints-224192015-10-05T08:17:12Z http://psasir.upm.edu.my/id/eprint/22419/ Isolation, cloning and characterisation of new fragrance-related floral transcripts of Vanda Mimi Palmer Chan, Wai Sun Abdullah, Janna Ong Namasivayam, Parameswari A subtracted cDNA library of open flower was constructed using the suppression subtractive hybridization (SSH) technique, to identify fragrance-related transcripts of Vanda Mimi Palmer. In total, 107 transcripts up-regulated during blooming were identified and sequenced. Only 33 clones (4 singletons and 29 contigs) showed similarities to known sequences in the public database. Of these, thirty-two clones were transcripts encoding fragrance-related enzymes including sesquiterpene synthase, (±)-germacrene D synthase, tyrosine decarboxylase and putative acyltransferase. Two fragrance-related transcripts, VMPAAT encoding a putative alcohol acyltransferase and VMPSTS encoding a sesquiterpene synthase, were subjected to full-length cDNA isolation and characterization. The full length cDNA of VMPAAT has a 1343bp open reading frame (ORF) of 448 amino acid residues whereas VMPSTS is predicted to encode a polypeptide of 561 amino acid residues with 1682bp ORF. VMPAAT and VMPSTS show high homologies with plant alcohol acyltransferase and terpene synthase, respectively. Real time RT-PCR indicated that both transcripts were expressed preferentially in floral tissues, with high levels in blooming and full bloom flowers. VMPAAT and VMPSTS transcripts were expressed in a rhythmic pattern. The results presented in this study will be potentially useful in providing additional insights into the fragrance-related pathways of Orchidaceae members, which until today is still limited. Elsevier 2011-01-10 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/22419/1/Isolation%2C%20cloning%20adn%20characteristion%20of%20new%20fragrance.pdf Chan, Wai Sun and Abdullah, Janna Ong and Namasivayam, Parameswari (2011) Isolation, cloning and characterisation of new fragrance-related floral transcripts of Vanda Mimi Palmer. Scientia Horticulturae, 127 (3). pp. 388-397. ISSN 0304-4238 http://www.sciencedirect.com/science/article/pii/S0304423810004474 10.1016/j.scienta.2010.09.024 |
| spellingShingle | Chan, Wai Sun Abdullah, Janna Ong Namasivayam, Parameswari Isolation, cloning and characterisation of new fragrance-related floral transcripts of Vanda Mimi Palmer |
| title | Isolation, cloning and characterisation of new fragrance-related floral transcripts of Vanda Mimi Palmer |
| title_full | Isolation, cloning and characterisation of new fragrance-related floral transcripts of Vanda Mimi Palmer |
| title_fullStr | Isolation, cloning and characterisation of new fragrance-related floral transcripts of Vanda Mimi Palmer |
| title_full_unstemmed | Isolation, cloning and characterisation of new fragrance-related floral transcripts of Vanda Mimi Palmer |
| title_short | Isolation, cloning and characterisation of new fragrance-related floral transcripts of Vanda Mimi Palmer |
| title_sort | isolation cloning and characterisation of new fragrance related floral transcripts of vanda mimi palmer |
| url | http://psasir.upm.edu.my/id/eprint/22419/1/Isolation%2C%20cloning%20adn%20characteristion%20of%20new%20fragrance.pdf |
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